Suppression of the onset and progression of collagen-induced arthritis by chebulagic acid screened from a natural product library
Language: 
English
Abstract: 

OBJECTIVE: Chebulagic acid (CHE) from the immature seeds of Terminalia chebula was identified from a natural product library as a potent suppressor of T cell activity. This study examined the effectiveness of CHE against the onset and progression of collagen-induced arthritis (CIA) in mice. METHODS: Arthritis was induced in DBA/1J mice by subcutaneous immunization with bovine type II collagen on days 0 and 21. CHE was administered intraperitoneally for 3 weeks, either as prophylaxis (10 or 20 mg/kg) before disease onset or as therapy (20 mg/kg) after disease onset. Clinical scores, serum antibody levels, and cytokines were measured, and flow cytometric analysis and real-time reverse transcription-polymerase chain reaction were performed to evaluate the knee joints of mice with CIA. RESULTS: In both the prophylactic and therapeutic CHE dosing models, all clinical scores, serum levels of total and anticollagen IgG, and levels of interleukin-10 (IL-10) and IL-6 were reduced, while serum levels of transforming growth factor beta (TGFbeta) were markedly elevated. The number of granulocytes was reduced, but the proportion of CD4+,CD25+ T cells was greater in the knee joints of CHE-treated CIA mice. Expression of Foxp3 and TGFbeta messenger RNA was also augmented significantly in the knee joints of CHE-treated CIA mice in the therapeutic dosing model. CONCLUSION: CHE significantly suppressed the onset and progression of CIA in mice. Immune suppression via the induction of TGFbeta and CD4+,CD25+ T cells may represent a new strategy in the development of therapies for managing rheumatoid arthritis and other inflammatory diseases.

Author(s): 
Lee, Sang-Ik
Hyun, Pung-Mi
Kim, Seung-Hyung
Kim, Kyoung-Shin
Lee, Sang-Keun
Kim, Byoung-Soo
Maeng, Pil Jae
Lim, Jong-Soon
Item Type: 
Journal Article
Publication Title: 
Arthritis and Rheumatism
Journal Abbreviation: 
Arthritis Rheum.
Publication Date: 
2005-01
Publication Year: 
2005
Pages: 
345-353
Volume: 
52
Issue: 
1
ISSN: 
0004-3591
DOI: 
10.1002/art.20715
Library Catalog: 
PubMed
Extra: 
PMID: 15641090

Turabian/Chicago Citation

Sang-Ik Lee, Pung-Mi Hyun, Seung-Hyung Kim, Kyoung-Shin Kim, Sang-Keun Lee, Byoung-Soo Kim, Pil Jae Maeng and Jong-Soon Lim. 2005-01. "Suppression of the onset and progression of collagen-induced arthritis by chebulagic acid screened from a natural product library." Arthritis and Rheumatism 52: 1: 345-353. 10.1002/art.20715.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1002/art.20715 | issn = 0004-3591 | volume = 52 | pages = 345-353 | last = Lee | first = Sang-Ik | coauthors = Hyun, Pung-Mi, Kim, Seung-Hyung, Kim, Kyoung-Shin, Lee, Sang-Keun, Kim, Byoung-Soo, Maeng, Pil Jae, Lim, Jong-Soon | title = Suppression of the onset and progression of collagen-induced arthritis by chebulagic acid screened from a natural product library | journal = Arthritis and Rheumatism | date = 2005-01 | pmid = | pmc = }} </ref>