SV40-induced immortalization of human cells
Language: 
English
Abstract: 

For several decades simian virus 40 (SV40) early region genes have been used as a means of generating immortalized human cell lines; however, the molecular mechanisms of this process have begun to be understood only recently. SV40-induced immortalization proceeds via two phases. In the first phase ("lifespan extension"), cells continue proliferating for a limited number of population doublings beyond the point at which normal cells undergo senescence. This is mainly due to the ability of SV40 large T antigen (LTAg) to bind to the protein products of the p53 and retinoblastoma (Rb) genes. The second phase ("immortalization") occurs in only a small minority of cells, and cell hybridization analyses indicate that this is a gene inactivation event. The gene or genes involved are currently unknown, but chromosomal localization data are accumulating which should make their cloning and characterization possible in the near future.

Author(s): 
Bryan, T. M.
Reddel, R. R.
Item Type: 
Journal Article
Publication Title: 
Critical Reviews in Oncogenesis
Journal Abbreviation: 
Crit Rev Oncog
Publication Date: 
1994
Publication Year: 
1994
Pages: 
331-357
Volume: 
5
Issue: 
4
ISSN: 
0893-9675
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 7711112

Turabian/Chicago Citation

T. M. Bryan and R. R. Reddel. 1994. "SV40-induced immortalization of human cells." Critical Reviews in Oncogenesis 5: 4: 331-357.

Wikipedia Citation

<ref> {{Cite journal | doi = | issn = 0893-9675 | volume = 5 | pages = 331-357 | last = Bryan | first = T. M. | coauthors = Reddel, R. R. | title = SV40-induced immortalization of human cells | journal = Critical Reviews in Oncogenesis | date = 1994 | pmid = | pmc = }} </ref>