SV40-mediated transformation and immortalization of human cells
Language: 
English
Abstract: 

SV40 infection of human cells results in both transformation and lytic infection. We have used origin-defective viral mutants which are unable to replicate in permissive cells to help analysis of transformation. Expression of large T antigen (T ag) and small t antigen results in the altered growth phenotypes characteristic of transformation in other species. Human diploid fibroblasts (HF) have a limited lifespan and undergo senescence; T ag results in extension of lifespan but only in rare cases are the cells capable of continuous growth and are immortal. We have developed matched sets of non-immortal and immortal transformed HF for assessment of the steps required for immortalization. Results are summarized to characterize both T-dependent and T-independent functions. A novel growth suppressor gene SEN6 has been identified, the inactivation of which is required for immortalization; it may also serve as a marker to distinguish cells in which SV40 is replicating from those in which it is responsible for tumorigenesis.

Author(s): 
Kim, S. H.
Banga, S.
Jha, K. K.
Ozer, H. L.
Item Type: 
Journal Article
Publication Title: 
Developments in Biological Standardization
Journal Abbreviation: 
Dev. Biol. Stand.
Publication Date: 
1998
Publication Year: 
1998
Pages: 
297-302
Volume: 
94
ISSN: 
0301-5149
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 9776250

Turabian/Chicago Citation

S. H. Kim, S. Banga, K. K. Jha and H. L. Ozer. 1998. "SV40-mediated transformation and immortalization of human cells." Developments in Biological Standardization 94: 297-302.

Wikipedia Citation

<ref> {{Cite journal | doi = | issn = 0301-5149 | volume = 94 | pages = 297-302 | last = Kim | first = S. H. | coauthors = Banga, S., Jha, K. K., Ozer, H. L. | title = SV40-mediated transformation and immortalization of human cells | journal = Developments in Biological Standardization | date = 1998 | pmid = | pmc = }} </ref>