SV40-mediated transformation and immortalization of human cells

SV40 infection of human cells results in both transformation and lytic infection. We have used origin-defective viral mutants which are unable to replicate in permissive cells to help analysis of transformation. Expression of large T antigen (T ag) and small t antigen results in the altered growth phenotypes characteristic of transformation in other species. Human diploid fibroblasts (HF) have a limited lifespan and undergo senescence; T ag results in extension of lifespan but only in rare cases are the cells capable of continuous growth and are immortal. We have developed matched sets of non-immortal and immortal transformed HF for assessment of the steps required for immortalization. Results are summarized to characterize both T-dependent and T-independent functions. A novel growth suppressor gene SEN6 has been identified, the inactivation of which is required for immortalization; it may also serve as a marker to distinguish cells in which SV40 is replicating from those in which it is responsible for tumorigenesis.

Kim, S. H.
Banga, S.
Jha, K. K.
Ozer, H. L.
Item Type: 
Journal Article
Publication Title: 
Developments in Biological Standardization
Journal Abbreviation: 
Dev. Biol. Stand.
Publication Date: 
Publication Year: 
Library Catalog: 
NCBI Published Medical (?)
PMID: 9776250

Turabian/Chicago Citation

S. H. Kim, S. Banga, K. K. Jha and H. L. Ozer. 1998. "SV40-mediated transformation and immortalization of human cells." Developments in Biological Standardization 94: 297-302.

Wikipedia Citation

<ref> {{Cite journal | doi = | issn = 0301-5149 | volume = 94 | pages = 297-302 | last = Kim | first = S. H. | coauthors = Banga, S., Jha, K. K., Ozer, H. L. | title = SV40-mediated transformation and immortalization of human cells | journal = Developments in Biological Standardization | date = 1998 | pmid = | pmc = }} </ref>