Synergistic anti-cancer activity of the combination of dihydroartemisinin and doxorubicin in breast cancer cells
Language: 
English
Abstract: 

BACKGROUND: Dihydroartemisinin (DHA) exhibits potent anti-malarial and anti-cancer activities. This study aimed to investigate the anti-proliferative effects of a combination of DHA and doxorubicin (DOX) on human breast cancer cells. METHODS: MTT assay and the combination index (CI) were used to show the anti-proliferative effects and calculate the synergism potential, respectively. Flow cytometry assay was used to detect apoptosis and the intracellular accumulation of DOX. JC-1 staining was used to determine the mitochondrial membrane potential. Western blot analysis was used to detect the protein expression of some apoptosis-related molecules. RESULTS: Asynergistic anti-proliferative effect was found, and the enhanced anti-cancer activity was observed to be accompanied by the prompt onset of apoptosis in MCF-7 cells. The combinative treatment remarkably decreased the mitochondrial membrane potential and activated caspase cascades more than the mono-treatment. Pretreatment with DHA also did not influence the accumulation of DOX in MCF-7 cells. CONCLUSION: This study presented a new opportunity to enhance the effectiveness of future treatment regimens of breast cancer using DOX.

Author(s): 
Wu, Guo-Sheng
Lu, Jin-Jian
Guo, Jia-Jie
Huang, Ming-Qing
Gan, Li
Chen, Xiu-Ping
Wang, Yi-Tao
Item Type: 
Journal Article
Publication Title: 
Pharmacological reports: PR
Journal Abbreviation: 
Pharmacol Rep
Publication Date: 
2013
Publication Year: 
2013
Pages: 
453-459
Volume: 
65
Issue: 
2
ISSN: 
1734-1140
Library Catalog: 
PubMed
Extra: 
PMID: 23744430

Turabian/Chicago Citation

Guo-Sheng Wu, Jin-Jian Lu, Jia-Jie Guo, Ming-Qing Huang, Li Gan, Xiu-Ping Chen and Yi-Tao Wang. 2013. "Synergistic anti-cancer activity of the combination of dihydroartemisinin and doxorubicin in breast cancer cells." Pharmacological reports: PR 65: 2: 453-459.

Wikipedia Citation

<ref> {{Cite journal | doi = | issn = 1734-1140 | volume = 65 | pages = 453-459 | last = Wu | first = Guo-Sheng | coauthors = Lu, Jin-Jian, Guo, Jia-Jie, Huang, Ming-Qing, Gan, Li, Chen, Xiu-Ping, Wang, Yi-Tao | title = Synergistic anti-cancer activity of the combination of dihydroartemisinin and doxorubicin in breast cancer cells | journal = Pharmacological reports: PR | date = 2013 | pmid = | pmc = }} </ref>