[Temperature-sensitive mutant of influenza virus: isolation and evaluation in mice]
Language: 
Chinese
Short Title: 
[Temperature-sensitive mutant of influenza virus
Abstract: 

A provisional temperature-sensitive mutant, ProTs8, of influenza A/WSN (H0N1) virus was isolated from primary chicken embryo fibroblast culture inoculated with influenza A/WSN (H0N1) and incubated with medium 199, containing 2% chicken embryo extract, and 400 micrograms/ml of 5-fluorouracil. Its EOP (efficiency of plaquing) value between 39.5 degrees C and 33 degrees C was about 0.007, and the control was about 0.82. Through lowering the shut-off-temperature to 38 degrees C, we had successfully isolated two temperature-sensitive mutants, Ts8-38 and Ts8-37, from their parent, ProTs8. 50% mouse virulent doses (MVD50) of these two mutants were around 10(-0.6), while the control group was 10(-4.0). Groups of mice immunized with 0.4 MVD50 or 0.04 MVD50 of Ts8-38 or Ts8-37 mutant by nasal instillation had shown significant resistance to the challenging infection of the wild type virus, influenza A/WSN (H0N1) (challenge dose, 1,000 MVD50 or 100 MVD50), if compared to the control group.

Author(s): 
Chen, S. H.
Tai, F. H.
Item Type: 
Journal Article
Publication Title: 
Zhonghua Minguo Wei Sheng Wu Ji Mian Yi Xue Za Zhi = Chinese Journal of Microbiology and Immunology
Journal Abbreviation: 
Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi
Publication Date: 
May 1983
Publication Year: 
1983
Pages: 
49-56
Volume: 
16
Issue: 
2
ISSN: 
0253-2662
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 6617313

Turabian/Chicago Citation

S. H. Chen and F. H. Tai. May 1983. "[Temperature-sensitive mutant of influenza virus: isolation and evaluation in mice]." Zhonghua Minguo Wei Sheng Wu Ji Mian Yi Xue Za Zhi = Chinese Journal of Microbiology and Immunology 16: 2: 49-56.

Wikipedia Citation

<ref> {{Cite journal | doi = | issn = 0253-2662 | volume = 16 | pages = 49-56 | last = Chen | first = S. H. | coauthors = Tai, F. H. | title = [Temperature-sensitive mutant of influenza virus: isolation and evaluation in mice] | journal = Zhonghua Minguo Wei Sheng Wu Ji Mian Yi Xue Za Zhi = Chinese Journal of Microbiology and Immunology | date = May 1983 | pmid = | pmc = }} </ref>