Terminalia chebula extract protects OGD-R induced PC12 cell death and inhibits lps induced microglia activation
Language: 
English
Abstract: 

Terminalia chebula, native to Southeast Asia, is a popular medicinal plant in Ayurveda. It has been previously reported to have strong antioxidant and anti-inflammatory efficacy. In this study, we aimed to investigate if fruit extract from T. chebula might protect neuronal cells against ischemia and related diseases by reduction of oxidative damage and inflammation in rat pheochromocytoma cells (PC12) using in vitro oxygen-glucose deprivation followed by reoxygenation (OGD-R) ischemia and hydrogen peroxide (H2O2) induced cell death. Cell survival was evaluated by a 2-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Free radical scavenging, lipid peroxidation and nitric oxide inhibition were measured by diphenyl-1-picrylhydrazyl (DPPH), thiobarbituric acid (TBA) and Griess reagent, respectively. We found that T. chebula extract: (1) increases the survival of cells subjected to OGD-R by 68%, and H2O2 by 91.4%; (2) scavenges the DPPH free radical by 96% and decreases malondialdehyde (MDA) levels from 237.0 ± 15.2% to 93.7 ± 2.2%; (3) reduces NO production and death rate of microglia cells stimulated by lipopolysaccharide (LPS). These results suggest that T. chebula extract has the potential as a natural herbal medicine, to protect the cells from ischemic damage and the possible mechanism might be the inhibition of oxidative and inflammatory processes.

Author(s): 
Gaire, Bhakta Prasad
Jamarkattel-Pandit, Nirmala
Lee, Donghun
Song, Jungbin
Kim, Ji Young
Park, Juyeon
Jung, Soyoung
Choi, Ho-Young
Kim, Hocheol
Item Type: 
Journal Article
Publication Title: 
Molecules (Basel, Switzerland)
Journal Abbreviation: 
Molecules
Publication Date: 
2013
Publication Year: 
2013
Pages: 
3529-3542
Volume: 
18
Issue: 
3
ISSN: 
1420-3049
DOI: 
10.3390/molecules18033529
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 23519197

Turabian/Chicago Citation

Bhakta Prasad Gaire, Nirmala Jamarkattel-Pandit, Donghun Lee, Jungbin Song, Ji Young Kim, Juyeon Park, Soyoung Jung, Ho-Young Choi and Hocheol Kim. 2013. "Terminalia chebula extract protects OGD-R induced PC12 cell death and inhibits lps induced microglia activation." Molecules (Basel, Switzerland) 18: 3: 3529-3542. 10.3390/molecules18033529.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.3390/molecules18033529 | issn = 1420-3049 | volume = 18 | pages = 3529-3542 | last = Gaire | first = Bhakta Prasad | coauthors = Jamarkattel-Pandit, Nirmala, Lee, Donghun, Song, Jungbin, Kim, Ji Young, Park, Juyeon, Jung, Soyoung, Choi, Ho-Young, Kim, Hocheol | title = Terminalia chebula extract protects OGD-R induced PC12 cell death and inhibits lps induced microglia activation | journal = Molecules (Basel, Switzerland) | date = 2013 | pmid = | pmc = }} </ref>