Toll-like receptor and innate cytokine responses induced by lactobacilli colonization and human rotavirus infection in gnotobiotic pigs
Language: 
English
Abstract: 

Toll-like receptors (TLR) play an important role in the recognition of microbes by host sentinel cells that leads to the subsequent innate and adaptive immune responses. In this study, we evaluated the patterns of TLR2-, TLR3- and TLR9-expressing antigen presenting cells (APCs) in spleen and blood of gnotobiotic (Gn) pigs after colonization with a mixture of two strains of lactic acid bacteria (LAB), Lactobacillus acidophilus and Lactobacillus reuteri or infection with the virulent human rotavirus (HRV) Wa strain. We also assessed the influence of LAB on TLR and serum innate cytokine responses induced by HRV. Distributions of subpopulations of APCs [CD14+/-SWC3+CD11R1- monocytes/macrophages and CD14+/-SWC3+CD11R1+ conventional dendritic cells (cDCs)] were described in our previous report (Zhang, W., Wen, K., Azevedo, M.S., Gonzalez, A.M., Saif, L.J., Li, G., Yousef, A.E., Yuan, L., 2008. Lactic acid bacterial colonization and human rotavirus infection influence distribution and frequencies of monocytes/macrophages and dendritic cells in neonatal gnotobiotic pigs. Vet. Immunol. Immunopathol. 121, pp. 222-231). We demonstrated that LAB induced strong TLR2-expressing APC responses in blood and spleen, HRV induced a TLR3 response in spleen, and TLR9 responses were induced by either HRV (in spleen) or LAB (in blood). LAB and HRV have an additive effect on TLR2- and TLR9-expressing APC responses, consistent with the adjuvant effect of LAB. Overall, the frequencies of TLR-expressing CD14+ APCs were higher than CD14- APCs. LAB enhanced the IFN-gamma and IL-4 responses in serum, but it had a suppressive effect on the TLR3- and TLR9-expressing CD14- APC responses in spleen and the serum IFN-alpha response induced by HRV. These results elucidated the systemic TLR2-, TLR3-, and TLR9-expressing monocyte/macrophage and cDC responses after HRV infection, LAB colonization, and the two combined. Our findings facilitate the understanding of the mechanism of LAB's adjuvant effect on rotavirus vaccines and the diverse innate and adaptive immune responses induced by commensal LAB colonization versus rotavirus infection and the interactions between them.

Author(s): 
Wen, Ke
Azevedo, Marli S. P.
Gonzalez, Ana
Zhang, Wei
Saif, Linda J.
Li, Guohua
Yousef, Ahmed
Yuan, Lijuan
Item Type: 
Journal Article
Publication Title: 
Veterinary Immunology and Immunopathology
Journal Abbreviation: 
Vet. Immunol. Immunopathol.
Publication Date: 
Feb 15, 2009
Publication Year: 
2009
Pages: 
304-315
Volume: 
127
Issue: 
3-4
ISSN: 
0165-2427
DOI: 
10.1016/j.vetimm.2008.10.322
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 19054578 PMCID: PMC2653198

Turabian/Chicago Citation

Ke Wen, Marli S. P. Azevedo, Ana Gonzalez, Wei Zhang, Linda J. Saif, Guohua Li, Ahmed Yousef and Lijuan Yuan. Feb 15, 2009. "Toll-like receptor and innate cytokine responses induced by lactobacilli colonization and human rotavirus infection in gnotobiotic pigs." Veterinary Immunology and Immunopathology 127: 3-4: 304-315. 10.1016/j.vetimm.2008.10.322.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1016/j.vetimm.2008.10.322 | issn = 0165-2427 | volume = 127 | pages = 304-315 | last = Wen | first = Ke | coauthors = Azevedo, Marli S. P., Gonzalez, Ana, Zhang, Wei, Saif, Linda J., Li, Guohua, Yousef, Ahmed, Yuan, Lijuan | title = Toll-like receptor and innate cytokine responses induced by lactobacilli colonization and human rotavirus infection in gnotobiotic pigs | journal = Veterinary Immunology and Immunopathology | date = Feb 15, 2009 | pmid = | pmc = }} </ref>