Tracer-based metabolomics: concepts and practices
Language: 
English
Short Title: 
Tracer-based metabolomics
Abstract: 

Tracer-based metabolomics is a systems biology tool that combines advances in tracer methodology for physiological studies, high throughput "-omics" technologies and constraint based modeling of metabolic networks. It is different from the commonly known metabolomics or metabonomics in that it is a targeted approach based on a metabolic network model in cells. Because of its complexity, it is the least understood among the various "-omics." In this review, the development of concepts and practices of tracer-based metabolomics is traced from the early application of radioactive isotopes in metabolic studies to the recent application of stable isotopes and isotopomer analysis using mass spectrometry; and from the modeling of biochemical reactions using flux analysis to the recent theoretical formulation of the constraint based modeling. How these newer experimental methods and concepts of constraint-based modeling approaches can be applied to metabolic studies is illustrated by examples of studies in determining metabolic responses of cells to pharmacological agents and nutrient environment changes.

Author(s): 
Paul Lee, W.-N.
Wahjudi, Paulin N.
Xu, Jun
Go, Vay Liang
Item Type: 
Journal Article
Publication Title: 
Clinical Biochemistry
Journal Abbreviation: 
Clin. Biochem.
Publication Date: 
Nov 2010
Publication Year: 
2010
Pages: 
1269-1277
Volume: 
43
Issue: 
16-17
ISSN: 
1873-2933
DOI: 
10.1016/j.clinbiochem.2010.07.027
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 20713038 PMCID: PMC2952699

Turabian/Chicago Citation

W.-N. Paul Lee, Paulin N. Wahjudi, Jun Xu and Vay Liang Go. Nov 2010. "Tracer-based metabolomics: concepts and practices." Clinical Biochemistry 43: 16-17: 1269-1277. 10.1016/j.clinbiochem.2010.07.027.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1016/j.clinbiochem.2010.07.027 | issn = 1873-2933 | volume = 43 | pages = 1269-1277 | last = Paul Lee | first = W.-N. | coauthors = Wahjudi, Paulin N., Xu, Jun, Go, Vay Liang | title = Tracer-based metabolomics: concepts and practices | journal = Clinical Biochemistry | date = Nov 2010 | pmid = | pmc = }} </ref>