Unravelling the retention of proliferation and differentiation potency in extensive culture of human subcutaneous fat-derived mesenchymal stem cells in different media
Language: 
English
Abstract: 

OBJECTIVES: This study has intended to investigate longevity of subcutaneous fat-derived mesenchymal stem cells (SF-MSCs) under extensive culturing. It has also focused on optimization of culture media for them over prolonged periods in vitro. MATERIALS AND METHODS: We evaluated SF-MSCs with reference to phenotypic characterization, proliferative ability, karyotype stability and differentiation potency with early (P3) and late passage (P20) conditions, using four different media, DMEM-LG, ALPHA-MEM, DMEM-F12 and DMEM-KO. RESULTS: This study unravels retention of SF-MSC characteristics in facets of phenotypic expression profile (CD 90, CD 105, CD 73, CD 34, CD 29, CD 54, CD 49d, CD 117, HLA-DR, CD 166, CD 31, CD 44), proliferative characteristics, karyotyping and differentiation potency prolonged culturing to P25 in all media. Population doubling time (PDT) in Alpha MEM, DMEM LG, DMEM F 12, DMEM KO were identified to be (1.81, 1.84, 1.9, 2.08†days) at early passage and (2.93, 2.94, 3.12, 3.06†days) at late passage. As a corollary, Alpha MEM and DMEM LG serve as appropriate basal media for SF-MSC when proliferative potency is considered. CONCLUSIONS: In research, it is imperative that SF-MSC uphold their expansion potency in the aforesaid attributes in all media over extensive culturing, thereby transforming their colossal in vitro potency, with the aim of curing a wide horizon of diseases.

Author(s): 
Dhanasekaran, M.
Indumathi, S.
Rashmi, M.
Rajkumar, J. S.
Sudarsanam, D.
Item Type: 
Journal Article
Publication Title: 
Cell Proliferation
Journal Abbreviation: 
Cell Prolif.
Publication Date: 
2012-12
Publication Year: 
2012
Pages: 
516-526
Volume: 
45
Issue: 
6
ISSN: 
1365-2184
DOI: 
10.1111/j.1365-2184.2012.00843.x
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 23106299

Turabian/Chicago Citation

M. Dhanasekaran, S. Indumathi, M. Rashmi, J. S. Rajkumar and D. Sudarsanam. 2012-12. "Unravelling the retention of proliferation and differentiation potency in extensive culture of human subcutaneous fat-derived mesenchymal stem cells in different media." Cell Proliferation 45: 6: 516-526. 10.1111/j.1365-2184.2012.00843.x.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1111/j.1365-2184.2012.00843.x | issn = 1365-2184 | volume = 45 | pages = 516-526 | last = Dhanasekaran | first = M. | coauthors = Indumathi, S., Rashmi, M., Rajkumar, J. S., Sudarsanam, D. | title = Unravelling the retention of proliferation and differentiation potency in extensive culture of human subcutaneous fat-derived mesenchymal stem cells in different media | journal = Cell Proliferation | date = 2012-12 | pmid = | pmc = }} </ref>