In vitro biophysical strain model for understanding mechanisms of osteopathic manipulative treatment
Language: 
English
Abstract: 

CONTEXT: Normal physiologic movement, pathologic conditions, and osteopathic manipulative treatment (OMT) are believed to produce effects on the shape and proliferation of human fibroblasts. Studies of biophysically strained fibroblasts would be useful in producing a model of the cellular mechanisms underlying OMT. OBJECTIVE: To investigate the effects of acyclic in vitro biophysical strain on normal human dermal fibroblasts and observe potential changes in cellular shape and proliferation, as well as potential changes in cellular production of nitric oxide, interleukin (IL) 1beta, and IL-6. DESIGN AND METHODS: Randomized controlled trial. Human fibroblasts were subjected in vitro to control conditions (no strain) or biophysical strain of various magnitudes (10%-30% beyond resting length) and durations (12-72 hours). After control or strain stimuli, fibroblasts were analyzed for potential changes in cell shape, proliferative capacity, nitric oxide secretion, and cytokine (IL-1beta, IL-6) secretion. RESULTS: Low strain magnitudes (<20%) induced mild cellular rounding and pseudopodia truncation. High strain magnitudes (>20%) decreased overall cell viability and the mitogenic response, and induced cell membrane decomposition and pseudopodia loss. No basal or strain-induced secretion of IL-1beta was observed. Interleukin 6 concentrations increased two-fold, while nitric oxide levels increased three-fold, in cells strained at 10% magnitude for 72 hours (P<.05). CONCLUSION: Human fibroblasts respond to in vitro strain by secreting inflammatory cytokines, undergoing hyperplasia, and altering cell shape and alignment. The in vitro biophysical strain model developed by the authors is useful for simulating a variety of injuries, determining in vivo mediators of somatic dysfunction, and investigating the underlying mechanisms of OMT.

Author(s): 
Dodd, John G.
Good, Meadow Maze
Nguyen, Tammy L.
Grigg, Andersen I.
Batia, Lyn M.
Standley, Paul R.
Item Type: 
Journal Article
Publication Title: 
The Journal of the American Osteopathic Association
Journal Abbreviation: 
J Am Osteopath Assoc
Publication Date: 
Mar 2006
Publication Year: 
2006
Pages: 
157-166
Volume: 
106
Issue: 
3
ISSN: 
0098-6151
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 16585384

Turabian/Chicago Citation

John G. Dodd, Meadow Maze Good, Tammy L. Nguyen, Andersen I. Grigg, Lyn M. Batia and Paul R. Standley. Mar 2006. "In vitro biophysical strain model for understanding mechanisms of osteopathic manipulative treatment." The Journal of the American Osteopathic Association 106: 3: 157-166.

Wikipedia Citation

<ref> {{Cite journal | doi = | issn = 0098-6151 | volume = 106 | pages = 157-166 | last = Dodd | first = John G. | coauthors = Good, Meadow Maze, Nguyen, Tammy L., Grigg, Andersen I., Batia, Lyn M., Standley, Paul R. | title = In vitro biophysical strain model for understanding mechanisms of osteopathic manipulative treatment | journal = The Journal of the American Osteopathic Association | date = Mar 2006 | pmid = | pmc = }} </ref>