Yeast culture supplement during nursing and transport affects immunity and intestinal microbial ecology of weanling pigs

The objectives of this study were to determine the influence of a Saccharomyces cerevisiae fermentation product on innate immunity and intestinal microbial ecology after weaning and transport stress. In a randomized complete block design, before weaning and in a split-plot analysis of a 2 × 2 factorial arrangement of yeast culture (YY) and transport (TT) after weaning, 3-d-old pigs (n = 108) were randomly assigned within litter (block) to either a control (NY, milk only) or yeast culture diet (YY; delivered in milk to provide 0.1 g of yeast culture product/kg of BW) from d 4 to 21. At weaning (d 21), randomly, one-half of the NY and YY pigs were assigned to a 6-h transport (NY-TT and YY-TT) before being moved to nursery housing, and the other one-half were moved directly to nursery housing (NY-NT and YY-NT, where NT is no transport). The yeast treatment was a 0.2% S. cerevisiae fermentation product and the control treatment was a 0.2% grain blank in feed for 2 wk. On d 1 before transport and on d 1, 4, 7, and 14 after transport, blood was collected for leukocyte assays, and mesenteric lymph node, jejunal, and ileal tissue, and jejunal, ileal, and cecal contents were collected for Toll-like receptor expression (TLR); enumeration of Escherichia coli, total coliforms, and lactobacilli; detection of Salmonella; and microbial analysis. After weaning, a yeast × transport interaction for ADG was seen (P = 0.05). Transport affected (P = 0.09) ADFI after weaning. Yeast treatment decreased hematocrit (P = 0.04). A yeast × transport interaction was found for counts of white blood cells (P = 0.01) and neutrophils (P = 0.02) and for the neutrophil-to-lymphocyte ratio (P = 0.02). Monocyte counts revealed a transport (P = 0.01) effect. Interactions of yeast × transport (P = 0.001) and yeast × transport × day (P = 0.09) for TLR2 and yeast × transport (P = 0.08) for TLR4 expression in the mesenteric lymph node were detected. Day affected lactobacilli, total coliform, and E. coli counts. More pigs were positive for Salmonella on d 7 and 14 than on d 4, and more YY-TT pigs were positive (P = 0.07) on d 4. The number of bands for microbial amplicons in the ileum was greater for pigs in the control treatment than in the yeast treatment on d 0, and this number tended to decrease (P = 0.066) between d 1 and 14 for all pigs. Similarity coefficients for jejunal contents were greater (P = 0.03) for pigs fed NY than for those fed YY, but pigs fed YY had greater similarity coefficients for ileal (P = 0.001) and cecal (P = 0.058) contents. The number of yeast × transport × day interactions demonstrates the complexity of the stress and dietary relationship.

Weedman, S. M.
Rostagno, M. H.
Patterson, J. A.
Yoon, I.
Fitzner, G.
Eicher, S. D.
Item Type: 
Journal Article
Publication Title: 
Journal of Animal Science
Journal Abbreviation: 
J. Anim. Sci.
Publication Date: 
Jun 2011
Publication Year: 
Library Catalog: 
NCBI Published Medical (?)
PMID: 21606447

Turabian/Chicago Citation

S. M. Weedman, M. H. Rostagno, J. A. Patterson, I. Yoon, G. Fitzner and S. D. Eicher. Jun 2011. "Yeast culture supplement during nursing and transport affects immunity and intestinal microbial ecology of weanling pigs." Journal of Animal Science 89: 6: 1908-1921. 10.2527/jas.2009-2539.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.2527/jas.2009-2539 | issn = 1525-3163 | volume = 89 | pages = 1908-1921 | last = Weedman | first = S. M. | coauthors = Rostagno, M. H., Patterson, J. A., Yoon, I., Fitzner, G., Eicher, S. D. | title = Yeast culture supplement during nursing and transport affects immunity and intestinal microbial ecology of weanling pigs | journal = Journal of Animal Science | date = Jun 2011 | pmid = | pmc = }} </ref>