3T3 Cells

Publication Title: 
Developments in Biological Standardization

SV40 infection of human cells results in both transformation and lytic infection. We have used origin-defective viral mutants which are unable to replicate in permissive cells to help analysis of transformation. Expression of large T antigen (T ag) and small t antigen results in the altered growth phenotypes characteristic of transformation in other species. Human diploid fibroblasts (HF) have a limited lifespan and undergo senescence; T ag results in extension of lifespan but only in rare cases are the cells capable of continuous growth and are immortal.

Author(s): 
Kim, S. H.
Banga, S.
Jha, K. K.
Ozer, H. L.
Publication Title: 
Aging Cell

Arsenite is one of the most toxic chemical substances known and is assumed to exert detrimental effects on viability even at lowest concentrations. By contrast and unlike higher concentrations, we here find that exposure to low-dose arsenite promotes growth of cultured mammalian cells. In the nematode C. elegans, low-dose arsenite promotes resistance against thermal and chemical stressors and extends lifespan of this metazoan, whereas higher concentrations reduce longevity. While arsenite causes a transient increase in reactive oxygen species (ROS) levels in C.

Author(s): 
Schmeisser, Sebastian
Schmeisser, Kathrin
Weimer, Sandra
Groth, Marco
Priebe, Steffen
Fazius, Eugen
Kuhlow, Doreen
Pick, Denis
Einax, J¸rgen W.
Guthke, Reinhard
Platzer, Matthias
Zarse, Kim
Ristow, Michael
Publication Title: 
Aging Cell

Arsenite is one of the most toxic chemical substances known and is assumed to exert detrimental effects on viability even at lowest concentrations. By contrast and unlike higher concentrations, we here find that exposure to low-dose arsenite promotes growth of cultured mammalian cells. In the nematode C. elegans, low-dose arsenite promotes resistance against thermal and chemical stressors and extends lifespan of this metazoan, whereas higher concentrations reduce longevity. While arsenite causes a transient increase in reactive oxygen species (ROS) levels in C.

Author(s): 
Schmeisser, Sebastian
Schmeisser, Kathrin
Weimer, Sandra
Groth, Marco
Priebe, Steffen
Fazius, Eugen
Kuhlow, Doreen
Pick, Denis
Einax, J¸rgen W.
Guthke, Reinhard
Platzer, Matthias
Zarse, Kim
Ristow, Michael
Publication Title: 
Circulation Research

Insulin promotes the cardiovascular protective functions of the endothelium including NO production by endothelial NO synthase (eNOS), which it stimulates via Akt kinase which phosphorylates eNOS Ser1179. C-reactive protein (CRP) is an acute-phase reactant that is positively correlated with cardiovascular disease risk in patients with type 2 diabetes. We previously showed that CRP inhibits eNOS activation by insulin by blunting Ser1179 phosphorylation. We now elucidate the underlying molecular mechanisms.

Author(s): 
Tanigaki, Keiji
Mineo, Chieko
Yuhanna, Ivan S.
Chambliss, Ken L.
Quon, Michael J.
Bonvini, Ezio
Shaul, Philip W.
Publication Title: 
Proceedings of the National Academy of Sciences of the United States of America

The breakdown of triglycerides, or lipolysis, is a tightly controlled process that regulates fat mobilization in accord with an animal's energy needs. It is well established that lipolysis is stimulated by hormones that signal energy demand and is suppressed by the antilipolytic hormone insulin. However, much still remains to be learned about regulation of lipolysis by intracellular signaling pathways in adipocytes.

Author(s): 
Yang, Ri-Yao
Yu, Lan
Graham, James L.
Hsu, Daniel K.
Lloyd, K. C. Kent
Havel, Peter J.
Liu, Fu-Tong
Publication Title: 
The Journal of Biological Chemistry

Rheumatoid arthritis (RA) is a chronic inflammatory disease characterized by bone erosion and cartilage destruction in the joints. Many of the conventional antiarthritic drugs are effective in suppressing inflammation, but they do not offer protection against bone damage. Furthermore, the prolonged use of these drugs is associated with severe adverse reactions. Thus, new therapeutic agents that can control both inflammation and bone damage but with minimal side effects are sought.

Author(s): 
Nanjundaiah, Siddaraju M.
Venkatesha, Shivaprasad H.
Yu, Hua
Tong, Li
Stains, Joseph P.
Moudgil, Kamal D.
Publication Title: 
Biological & Pharmaceutical Bulletin

Chikusaku-eki is an acidic dark brown liquid obtained as a by-product from bamboo charcoal burners. The solution diluted with water is gaining widespread popularity in Japan as a folk medicine for skin diseases such as scabies, eczema, and atopic dermatitis. In this study, the carcinogenic and tumor-promoting potential of chikusaku-eki was determined using the BALB/c 3T3 A31-1-1 cell transformation system. Carcinogenic activity was tested by treating A31-1-1 cells for 24 h with 0.06% solution, a dose resulting in 35% clonogenic cell survival.

Author(s): 
Kimura, Yuki
Suto, Shiho
Tatsuka, Masaaki
Publication Title: 
The Journal of Biological Chemistry

Rheumatoid arthritis (RA) is a chronic inflammatory disease characterized by bone erosion and cartilage destruction in the joints. Many of the conventional antiarthritic drugs are effective in suppressing inflammation, but they do not offer protection against bone damage. Furthermore, the prolonged use of these drugs is associated with severe adverse reactions. Thus, new therapeutic agents that can control both inflammation and bone damage but with minimal side effects are sought.

Author(s): 
Nanjundaiah, Siddaraju M.
Venkatesha, Shivaprasad H.
Yu, Hua
Tong, Li
Stains, Joseph P.
Moudgil, Kamal D.
Publication Title: 
The Journal of Clinical Investigation

The ability to regulate specific genes of energy metabolism in response to fasting and feeding is an important adaptation allowing survival of intermittent food supplies. However, little is known about transcription factors involved in such responses in higher organisms.

Author(s): 
Kim, J. B.
Sarraf, P.
Wright, M.
Yao, K. M.
Mueller, E.
Solanes, G.
Lowell, B. B.
Spiegelman, B. M.
Publication Title: 
The Journal of Biological Chemistry

Galectins constitute a family of proteins that bind to beta-galactoside residues and have diverse physiological functions. Here we report on the identification of a galectin-like molecule, galectin-12, in a human adipose tissue cDNA library. The protein contained two potential carbohydrate-recognition domains with the second carbohydrate-recognition domain being less conserved compared with other galectins. In vitro translated galectin-12 bound to a lactosyl-agarose column far less efficiently than galectin-8.

Author(s): 
Hotta, K.
Funahashi, T.
Matsukawa, Y.
Takahashi, M.
Nishizawa, H.
Kishida, K.
Matsuda, M.
Kuriyama, H.
Kihara, S.
Nakamura, T.
Tochino, Y.
Bodkin, N. L.
Hansen, B. C.
Matsuzawa, Y.

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