Acclimatization

Publication Title: 
Plant Cell Reports

Resistant rootstocks offer an alternative to pesticides for the control of soil pests. In Prunus spp., resistance loci to root-knot nematodes (RKN) have been mapped and a transformation method is needed to validate candidate genes. Our efforts have focused on the generation of transformed hairy-roots and composite plants appropriate for nematode infection assays. An efficient and reliable method using the A4R strain of Agrobacterium rhizogenes for the transformation of Prunus roots with an Egfp reporter gene is given.

Author(s): 
Bosselut, Nathalie
Van Ghelder, Cyril
Claverie, Michel
Voisin, Roger
Onesto, Jean-Paul
Rosso, Marie-Noëlle
Esmenjaud, Daniel
Publication Title: 
Journal of Applied Physiology
Author(s): 
Kissen, A. T.
Reifler, C. B.
Thaler, V. H.
Publication Title: 
The Journal of Nutrition

We evaluated the role of dietary phytoestrogens (PE) in the disease phenotype of cold intolerance that characterizes long-chain acyl-CoA dehydrogenase-deficient (LCAD-/-) mice, a model of inborn errors of mitochondrial fatty acid beta-oxidation. Male LCAD-/- mice were fed a standard diet containing endogenous PE, a PE-free diet, or a PE-free diet that was supplemented with genistein (250 microg/g diet). The standard diet did not restore complete cold tolerance, but it provided more resistance (P = 0.004) to cold challenge than the PE-free diet.

Author(s): 
Schuler, A. Michele
Barnes, Stephen
Gower, Barbara A.
Wood, Philip A.
Publication Title: 
Cell Metabolism

G(s)alpha, the G protein that mediates receptor-stimulated cAMP generation, has been implicated as a regulator of adipogenesis and adipose tissue function. Heterozygous G(s)alpha mutations lead to obesity in Albright hereditary osteodystrophy (AHO) patients and in mice. In this study, we generated mice with adipose-specific G(s)alpha deficiency. Heterozygotes had 50% loss of G(s)alpha expression in adipose tissue and no obvious phenotype, suggesting that adipose-specific G(s)alpha deficiency is not the cause of obesity in AHO.

Author(s): 
Chen, Min
Chen, Hui
Nguyen, Annie
Gupta, Divakar
Wang, Jie
Lai, Edwin W.
Pacak, Karel
Gavrilova, Oksana
Quon, Michael J.
Weinstein, Lee S.
Publication Title: 
Anais Da Academia Brasileira De Ciências

This work describes an efficient micropropagation protocol for Verbena litoralis and the study of the antinociceptive and antioxidant activities in extracts of this species. For the establishment in vitro, surface-sterilization procedures and PVPP showed high efficiency in fungal-bacterial contamination and phenol oxidation controls. Nodal segments cultivation in MS medium supplemented with 6-benzyladenine (7.5 µM)/α-naphthaleneacetic acid (NAA; 0.005 µM) induced multiple shoots. Elongated shoots were rooted with IAA (0.2 µM).

Author(s): 
Braga, Virgínia F.
Mendes, Giselle C.
Oliveira, Raphael T. R.
Soares, Carla Q. G.
Resende, Cristiano F.
Pinto, Leandro C.
Santana, Reinaldo de
Viccini, Lyderson F.
Raposo, Nádia R. B.
Peixoto, Paulo H. P.
Publication Title: 
Journal of Manipulative and Physiological Therapeutics

BACKGROUND: Paraspinal thermography has been used by chiropractors since 1924. One method of its interpretation is with the use of "pattern analysis"-a method that assesses temperature differentials (patterns). This, in turn, theoretically provides information about nervous system function. When a warm back is exposed to the cooler air in the examining room, the skin temperature, in general, drops but the differentials could remain fairly constant. OBJECTIVE: To determine what changes occur in paraspinal heat patterns when the back is exposed to room temperature.

Author(s): 
Hart, John
Owens, Edward F.
Publication Title: 
Journal of Manipulative and Physiological Therapeutics

BACKGROUND: Paraspinal thermography has been used by chiropractors since 1924. One method of its interpretation is with the use of "pattern analysis"-a method that assesses temperature differentials (patterns). This, in turn, theoretically provides information about nervous system function. When a warm back is exposed to the cooler air in the examining room, the skin temperature, in general, drops but the differentials could remain fairly constant. OBJECTIVE: To determine what changes occur in paraspinal heat patterns when the back is exposed to room temperature.

Author(s): 
Hart, John
Owens, Edward F.
Publication Title: 
Journal of Manipulative and Physiological Therapeutics

OBJECTIVE: Digitized infrared segmental thermometry (DIST) is a tool used for measuring cutaneous temperature (CT). This project ascertains the effect of a manually assisted mechanical force producing a chiropractic adjustment in the lumbar spine after the Activator Methods Chiropractic Technique on CT during 2 different time recording periods (TRPs). METHODS: Sixty-six healthy subjects (36 women and 30 men) without acute low back conditions or symptoms were recruited.

Author(s): 
Roy, Richard A.
Boucher, Jean P.
Comtois, Alain S.
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