Adenoviridae

Publication Title: 
International Journal of Oncology

Granulocyte macrophage colony-stimulating factor (GM-CSF) is a key cytokine for the generation and stimulation of dendritic cells (DCs), and it may also play a pivotal role in promoting the survival of DCs. In this study, the feasibility of creating a cancer vaccine using DCs adenovirally transduced with the carcinoembryonic antigen (CEA) gene and the GM-CSF gene was examined. In addition, the effect of the co-transduction of GM-CSF gene on the lifespan of these genetically modified DCs was determined.

Author(s): 
Ojima, Toshiyasu
Iwahashi, Makoto
Nakamura, Masaki
Matsuda, Kenji
Nakamori, Mikihito
Ueda, Kentaro
Naka, Teiji
Katsuda, Masahiro
Miyazawa, Motoki
Yamaue, Hiroki
Publication Title: 
Experimental & Molecular Medicine

Neural progenitor cells (NPs) have shown several promising benefits for the treatment of neurological disorders. To evaluate the therapeutic potential of human neural progenitor cells (hNPs) in amyotrophic lateral sclerosis (ALS), we transplanted hNPs or growth factor (GF)-expressing hNPs into the central nervous system (CNS) of mutant Cu/Zn superoxide dismutase (SOD1(G93A)) transgenic mice.

Author(s): 
Park, Sungju
Kim, Hyoung-Tae
Yun, Seokkwan
Kim, Il-Sun
Lee, Jiyoon
Lee, Il-Shin
Park, Kook In
Publication Title: 
American Journal of Physiology. Heart and Circulatory Physiology

In the present study, we examined whether NF-kappaB activation is required for cardiac hypertrophy in vivo. Cardiac hypertrophy in rats was induced by aortic banding for 1, 3, and 5 days and 1-6 wk, and age-matched sham-operated rats served as controls. In a separate group of rats, an IkappaB-alpha dominant negative mutant (IkappaB-alphaM), a superrepressor of NF-kappaB activation, or pyrrolidinedithiocarbamate (PDTC), an antioxidant that can inhibit NF-kappaB activation, was administered to aortic-banded rats for 3 wk.

Author(s): 
Li, Yuehua
Ha, Tuanzhu
Gao, Xiang
Kelley, Jim
Williams, David L.
Browder, I. William
Kao, Race L.
Li, Chuanfu
Publication Title: 
The Journal of Biological Chemistry

Bone morphogenetic proteins (BMPs) belong to the TGF-beta superfamily and play an important role in development and in many cellular processes. We have found that BMP-2, BMP-6, and BMP-9 induce the most potent osteogenic differentiation of mesenchymal stem cells. Expression profiling analysis has revealed that the Inhibitors of DNA binding/differentiation (Id)-1, Id-2, and Id-3 are among the most significantly up-regulated genes upon BMP-2, BMP-6, or BMP-9 stimulation. Here, we sought to determine the functional role of these Id proteins in BMP-induced osteoblast differentiation.

Author(s): 
Peng, Ying
Kang, Quan
Luo, Qing
Jiang, Wei
Si, Weike
Liu, Bernard A.
Luu, Hue H.
Park, Jong Kyung
Li, Xinmin
Luo, Jeffrey
Montag, Anthony G.
Haydon, Rex C.
He, Tong-Chuan
Publication Title: 
Molecular Biology of the Cell

Increased expression of vascular endothelial growth factor (VEGF) contributes to the growth of many tumors by increasing angiogenesis. Although hypoxia is a potent inducer of VEGF, we previously showed that epidermal growth factor receptor amplification and loss of PTEN, both of which can increase phosphatidylinositol-3-kinase (PI3K) activity, increase VEGF expression.

Author(s): 
Pore, Nabendu
Liu, Shuang
Shu, Hui-Kuo
Li, Bin
Haas-Kogan, Daphne
Stokoe, David
Milanini-Mongiat, Julie
Pages, Gilles
O'Rourke, Donald M.
Bernhard, Eric
Maity, Amit
Publication Title: 
The Journal of Biological Chemistry

Osteoblast lineage-specific differentiation of mesenchymal stem cells is a well regulated but poorly understood process. Both bone morphogenetic proteins (BMPs) and Wnt signaling are implicated in regulating osteoblast differentiation and bone formation. Here we analyzed the expression profiles of mesenchymal stem cells stimulated with Wnt3A and osteogenic BMPs, and we identified connective tissue growth factor (CTGF) as a potential target of Wnt and BMP signaling.

Author(s): 
Luo, Qing
Kang, Quan
Si, Weike
Jiang, Wei
Park, Jong Kyung
Peng, Ying
Li, Xinmin
Luu, Hue H.
Luo, Jeffrey
Montag, Anthony G.
Haydon, Rex C.
He, Tong-Chuan
Publication Title: 
Diabetes

OBJECTIVE: Experimental infection of rats with human adenovirus type 36 (Ad-36) promotes adipogenesis and improves insulin sensitivity in a manner reminiscent of the pharmacologic effect of thiozolinediones. To exploit the potential of the viral proteins as a therapeutic target for treating insulin resistance, this study investigated the ability of Ad-36 to induce metabolically favorable changes in human adipose tissue. RESEARCH DESIGN AND METHODS: We determined whether Ad-36 increases glucose uptake in human adipose tissue explants.

Author(s): 
Rogers, Pamela M.
Mashtalir, Nazar
Rathod, Miloni A.
Dubuisson, Olga
Wang, Zhong
Dasuri, Kumar
Babin, Scott
Gupta, Alok
Markward, Nathan
Cefalu, William T.
Dhurandhar, Nikhil V.
Publication Title: 
Cardiovascular Research

AIMS: Nuclear factor-kappaB (NF-kappaB) plays a critical role in cell growth and inflammation during the progression of cardiac hypertrophy and heart failure. Several members of nuclear receptor superfamily, including liver X receptors (LXRalpha and LXRbeta), have been shown to suppress inflammatory responses, but little is known about their effects in cardiomyocytes. METHODS AND RESULTS: We investigated LXR expression patterns in pressure overload-induced hypertrophic hearts and the hypertrophic growth of the LXRalpha-deficient hearts from mice (C57/B6) in response to pressure overload.

Author(s): 
Wu, Sijie
Yin, Ran
Ernest, Rick
Li, Yuquan
Zhelyabovska, Olga
Luo, Jinwen
Yang, Yifeng
Yang, Qinglin
Publication Title: 
Cardiovascular & Hematological Disorders Drug Targets

Previous studies using different techniques have shown that adenoviral-mediated gene transfer to different tissues, including the kidney, is more efficient in neonatal mice. In this study, we report a simple technique that allows an efficient and long term expression of beta-galactosidase (beta-gal) in the heart of newborn mice. Newborn and adult C57BL6/J mice were subjected to a single retro-orbital venous plexus injection of recombinant adenoviral vectors (rAd) (2 x 10(9) particles/g body weight) carrying the lac Z gene.

Author(s): 
Jerebtsova, Marina
Ye, Xuehai
Ray, Patricio E.
Publication Title: 
The Journal of Biological Chemistry

Loss of ABCA1 activity in Tangier disease (TD) is associated with abnormal apoB lipoprotein (Lp) metabolism in addition to the complete absence of high density lipoprotein (HDL). We used hepatocyte-specific ABCA1 knock-out (HSKO) mice to test the hypothesis that hepatic ABCA1 plays dual roles in regulating Lp metabolism and nascent HDL formation. HSKO mice recapitulated the TD lipid phenotype with postprandial hypertriglyceridemia, markedly decreased LDL, and near absence of HDL.

Author(s): 
Chung, Soonkyu
Timmins, Jenelle M.
Duong, Myngan
Degirolamo, Chiara
Rong, Shunxing
Sawyer, Janet K.
Singaraja, Roshni R.
Hayden, Michael R.
Maeda, Nobuyo
Rudel, Lawrence L.
Shelness, Gregory S.
Parks, John S.

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