Annexin A5

Publication Title: 
Biophysical Journal

In contrast with most inhalational anesthetics, the anesthetic gases xenon (Xe) and nitrous oxide (N(2)O) act by blocking the N-methyl-d-aspartate (NMDA) receptor. Using x-ray crystallography, we examined the binding characteristics of these two gases on two soluble proteins as structural models: urate oxidase, which is a prototype of a variety of intracellular globular proteins, and annexin V, which has structural and functional characteristics that allow it to be considered as a prototype for the NMDA receptor. The structure of these proteins complexed with Xe and N(2)O were determined.

Author(s): 
Colloc'h, Nathalie
Sopkova-de Oliveira Santos, Jana
Retailleau, Pascal
Vivarès, Denis
Bonneté, Françoise
Langlois d'Estainto, Béatrice
Gallois, Bernard
Brisson, Alain
Risso, Jean-Jacques
Lemaire, Marc
Prangé, Thierry
Abraini, Jacques H.
Publication Title: 
Tumori

AIMS AND BACKGROUND: Previous studies showed that dihydroartemisinin (DHA) possessed antitumor activity in many human tumor cells through the induction of apoptosis. The aim of this study was to investigate the effects of DHA on apoptosis in the human hepatocellular carcinoma cell line HepG2 and the possible molecular mechanisms involved. METHODS: The inhibitory effect of DHA on HepG2 cells was measured by MTT assay. The percentage of apoptotic cells was detected by flow cytometry with double staining of fluorescein isothiocyanate-annexin V/propidium iodide.

Author(s): 
Gao, Xiaoling
Luo, Ziguo
Xiang, Tingxiu
Wang, Kejian
Li, Jian
Wang, Pilong
Publication Title: 
PloS One

Artemether is the derivative extracted from Chinese traditional herb and originally used for malaria. Artemether also has potential therapeutic effects against tumors. Vascular cell adhesion molecule-1 (VCAM-1) is an important cell surface adhesion molecule associated with malignancy of gliomas. In this work, we investigated the role and mechanism of artemether combined with shRNA interference of VCAM-1 (shRNA-VCAM-1) on the migration, invasion and apoptosis of glioma cells.

Author(s): 
Wang, Ying-Bin
Hu, Yi
Li, Zhen
Wang, Ping
Xue, Yi-Xue
Yao, Yi-Long
Yu, Bo
Liu, Yun-Hui
Publication Title: 
BMC complementary and alternative medicine

BACKGROUND: Inhibition of the proteolytic activity of 26S proteasome, the protein-degrading machine, is now considered a novel and promising approach for cancer therapy. Interestingly, proteasome inhibitors have been demonstrated to selectively kill cancer cells and also enhance the sensitivity of tumor cells to chemotherapeutic agents. Recently, polyphenols/flavonoids have been reported to inhibit proteasome activity. Murraya koenigii Spreng, a medicinally important herb of Indian origin, has been used for centuries in the Ayurvedic system of medicine.

Author(s): 
Noolu, Bindu
Ajumeera, Rajanna
Chauhan, Anitha
Nagalla, Balakrishna
Manchala, Raghunath
Ismail, Ayesha
Publication Title: 
Cancer Letters

The cytotoxic effects of aqueous extract of Triphala, an ayurvedic formulation, were investigated on human breast cancer cell line (MCF-7) and a transplantable mouse thymic lymphoma (barcl-95). The viability of treated cells was found to decrease with the increasing concentrations of Triphala. On the other hand, treatment of normal breast epithelial cells, MCF-10 F, human peripheral blood mononuclear cells, mouse liver and spleen cells, with similar concentrations of Triphala did not affect their cytotoxicity significantly.

Author(s): 
Sandhya, T.
Lathika, K. M.
Pandey, B. N.
Mishra, K. P.
Publication Title: 
Phytotherapy research: PTR

The Ayurvedic system of medicine recommends Bacopa monniera (BM) in the treatment of tumors. The present study aims to determine the mode of cell death induced by the ethanolic extract of BM in mouse S-180 cells. BM-treated S-180 cells were assessed for cell viability in a dose- and time-dependent manner using dye exclusion studies. Morphological changes in the BM-treated and untreated cells were studied by transmission electron microscopy (TEM). Glutathione (GSH) levels were quantified and the percentage of apoptotic cells was determined using Annexin V-FITC assay.

Author(s): 
Rohini, G.
Devi, C. S. Shyamala
Publication Title: 
Journal of Natural Medicines

Njavara is an important medicinal rice variety of Kerala, India widely used in Ayurveda for the treatment of rheumatoid arthritis, paralysis, neurodegenerative diseases and in rejuvenation therapy. The study evaluated, for the first time, antitumor effects of the two rare flavonolignans, tricin 4'-O-(erythro-β-guaiacylglyceryl) ether (compound 1) and tricin 4'-O-(threo-β-guaiacylglyceryl) ether (compound 2), isolated from 'Njavara' black.

Author(s): 
Mohanlal, Smitha
Maney, Sathish Kumar
Santhoshkumar, Thankayyan Retnabai
Jayalekshmy, Ananthasankaran
Publication Title: 
BMC complementary and alternative medicine

BACKGROUND: Inhibition of the proteolytic activity of 26S proteasome, the protein-degrading machine, is now considered a novel and promising approach for cancer therapy. Interestingly, proteasome inhibitors have been demonstrated to selectively kill cancer cells and also enhance the sensitivity of tumor cells to chemotherapeutic agents. Recently, polyphenols/flavonoids have been reported to inhibit proteasome activity. Murraya koenigii Spreng, a medicinally important herb of Indian origin, has been used for centuries in the Ayurvedic system of medicine.

Author(s): 
Noolu, Bindu
Ajumeera, Rajanna
Chauhan, Anitha
Nagalla, Balakrishna
Manchala, Raghunath
Ismail, Ayesha
Publication Title: 
Cell Proliferation

OBJECTIVES: Gymnema montanum Hook, an Indian Ayurvedic medicinal plant, is used traditionally to treat a variety of ailments. Here, we report anti-cancer effects and molecular mechanisms of ethanolic extract of G. montanum (GLEt) on human leukaemia HL-60 cells, compared to peripheral blood mononuclear cells. MATERIALS AND METHODS: HL-60 cells were treated with different concentrations of GLEt (10-50 μg/ml) and cytotoxicity was assessed by MTT assay. Levels of lipid peroxidation, antioxidants, mitochondrial membrane potential and caspase-3 were measured.

Author(s): 
Ramkumar, K. M.
Manjula, C.
Elango, B.
Krishnamurthi, K.
Saravana Devi, S.
Rajaguru, P.
Publication Title: 
Food and Chemical Toxicology: An International Journal Published for the British Industrial Biological Research Association

The rhizome of Curcuma longa (CL) has been commonly used in Asia as a potential candidate for the treatment of different diseases, including inflammatory disorders and cancers. The present study evaluated the anti-proliferative activities of the isolated compounds (three curcuminoids and two turmerones) from CL, using human cancer cell lines HepG2, MCF-7 and MDA-MB-231. The immunomodulatory activities of turmerones (alpha and aromatic) isolated from CL were also examined using human peripheral blood mononuclear cells (PBMC).

Author(s): 
Yue, Grace G. L.
Chan, Ben C. L.
Hon, Po-Ming
Lee, Mavis Y. H.
Fung, Kwok-Pui
Leung, Ping-Chung
Lau, Clara B. S.

Pages

Subscribe to RSS - Annexin A5