Arabidopsis Proteins

Publication Title: 
Aging Cell

Caloric restriction (CR) extends lifespan in various heterotrophic organisms ranging from yeasts to mammals, but whether a similar phenomenon occurs in plants remains unknown. Plants are autotrophs and use their photosynthetic machinery to convert light energy into the chemical energy of glucose and other organic compounds. As the rate of photosynthesis is proportional to the level of photosynthetically active radiation, the CR in plants can be modeled by lowering light intensity.

Author(s): 
Minina, Elena A.
Sanchez-Vera, Victoria
Moschou, Panagiotis N.
Suarez, Maria F.
Sundberg, Eva
Weih, Martin
Bozhkov, Peter V.
Publication Title: 
PloS One

The phytohormone abscisic acid (ABA) plays an important role in plant development and environmental stress response. In this study, we cloned an ABA receptor orthologue, AaPYL9, from Artemisia annua L. AaPYL9 is expressed highly in leaf and flower. AaPYL9 protein can be localized in both nucleus and cytoplasm. Yeast two-hybrid assay shows AaPYL9 can specifically interact with AtABI1 but not with AtABI2, AtHAB1 or AtHAB2. ABA can enhance the interaction between AaPYL9 and AtABI1 while AaPYL9-89 Pro?Ser and AaPYL9-116 His?Ala point mutations abolishes the interaction.

Author(s): 
Zhang, Fangyuan
Lu, Xu
Lv, Zongyou
Zhang, Ling
Zhu, Mengmeng
Jiang, Weiming
Wang, Guofeng
Sun, Xiaofen
Tang, Kexuan
Publication Title: 
The Plant Cell

Plant phototropism is an adaptive response to changes in light direction, quantity, and quality that results in optimization of photosynthetic light harvesting, as well as water and nutrient acquisition. Though several components of the phototropic signal response pathway have been identified in recent years, including the blue light (BL) receptors phototropin1 (phot1) and phot2, much remains unknown. Here, we show that the phot1-interacting protein NONPHOTOTROPIC HYPOCOTYL3 (NPH3) functions as a substrate adapter in a CULLIN3-based E3 ubiquitin ligase, CRL3(NPH3).

Author(s): 
Roberts, Diana
Pedmale, Ullas V.
Morrow, Johanna
Sachdev, Shrikesh
Lechner, Esther
Tang, Xiaobo
Zheng, Ning
Hannink, Mark
Genschik, Pascal
Liscum, Emmanuel
Publication Title: 
Aging Cell

Caloric restriction (CR) extends lifespan in various heterotrophic organisms ranging from yeasts to mammals, but whether a similar phenomenon occurs in plants remains unknown. Plants are autotrophs and use their photosynthetic machinery to convert light energy into the chemical energy of glucose and other organic compounds. As the rate of photosynthesis is proportional to the level of photosynthetically active radiation, the CR in plants can be modeled by lowering light intensity.

Author(s): 
Minina, Elena A.
Sanchez-Vera, Victoria
Moschou, Panagiotis N.
Suarez, Maria F.
Sundberg, Eva
Weih, Martin
Bozhkov, Peter V.
Publication Title: 
Photosynthesis Research

Rubisco frequently undergoes unproductive interactions with its sugar-phosphate substrate that stabilize active sites in an inactive conformation. Restoring catalytic competence to these sites requires the "molecular chiropractic" activity of Rubisco activase (activase). To make the study of activase more routine and physiologically relevant, an assay was devised for measuring activase activity in leaf extracts based on the ATP-dependent activation of inactive Rubisco.

Author(s): 
Carmo-Silva, A. Elizabete
Salvucci, Michael E.
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