Bacterial Proteins

Publication Title: 
Plant Cell Reports

Resistant rootstocks offer an alternative to pesticides for the control of soil pests. In Prunus spp., resistance loci to root-knot nematodes (RKN) have been mapped and a transformation method is needed to validate candidate genes. Our efforts have focused on the generation of transformed hairy-roots and composite plants appropriate for nematode infection assays. An efficient and reliable method using the A4R strain of Agrobacterium rhizogenes for the transformation of Prunus roots with an Egfp reporter gene is given.

Author(s): 
Bosselut, Nathalie
Van Ghelder, Cyril
Claverie, Michel
Voisin, Roger
Onesto, Jean-Paul
Rosso, Marie-Noëlle
Esmenjaud, Daniel
Publication Title: 
Systematic and Applied Microbiology

Five Gram-negative, rod-shaped, non-spore-forming bacteria were isolated from galls on different plant species in Hungary: strain 39/7(T) from Prunus cerasifera Myrobalan, strain 0 from grapevine var. Ezerjó, strain 7/1 from raspberry var. Findus and in Poland, strain C3.4.1 from Colt rootstock (Prunus avium × Prunus pseudocerasus) and strain CP17.2.2 from Prunus avium. Only one of these isolates, strain 0, is able to cause crown gall on different plant species.

Author(s): 
Pu?awska, Joanna
Willems, Anne
De Meyer, Sofie E.
Süle, Sandor
Publication Title: 
Pharmaceutical Biology

CONTEXT: Bacterial ureases play an important role in pathogenesis of urinary infections. Selection of plants was done on the basis of their uses by the local people for the treatment of various bacterial and urinary infections. OBJECTIVE: Our investigation screens and evaluates 15 Indian medicinal plants for their possible urease inhibitory activity as well as their ability to inhibit bacteria causing urinary infections.

Author(s): 
Bai, Sheema
Bharti, Pooja
Seasotiya, Leena
Malik, Anupma
Dalal, Sunita
Publication Title: 
Journal of Applied Microbiology

AIM: Polyphosphate kinase 1 (PPK1) plays an important role in virulence, antibiotic resistance and survival under stress conditions and, therefore, is an attractive therapeutic target to control infections caused by multiple drug resistant Pseudomonas aeruginosa. This study explores the PPK1 inhibiting activity of ellagic acid derivatives (EADs) from Terminalia chebula Retz. that could increase the susceptibility of Ps. aeruginosa to in vitro stress conditions.

Author(s): 
Sarabhai, S.
Harjai, K.
Sharma, P.
Capalash, N.
Publication Title: 
Journal of Biomolecular Structure & Dynamics

The emergence of bacterial multidrug resistance is an increasing problem in treatment of infectious diseases. An important cause for the multidrug resistance of bacteria is the expression of multidrug efflux transporters. The multidrug and toxic compound extrusion (MATE) transporters are most recently recognized as unique efflux system for extrusion of antimicrobials and therapeutic drugs due to energy stored in either Na(+) or H(+) electrochemical gradient.

Author(s): 
Kesherwani, Manish
Michael Gromiha, M.
Fukui, Kazuhiko
Velmurugan, Devadasan
Publication Title: 
PloS One

Evaluating ecological safety and conducting pest risk analysis for transgenic crops are vitally important before their commercial planting. The beet armyworm, Spodoptera exigua, a long-distance migratory insect pest, is not a direct target of transgenic Cry1Ac-expressing cotton in China, but nevertheless it has recently become an important pest. Migrants leaving their natal field arrive in other appropriate habitat far away in a short time, often followed by larval outbreaks. S. exigua has low susceptibility to Cry1Ac.

Author(s): 
Jiang, Xing Fu
Chen, Jian
Zhang, Lei
Sappington, Thomas W.
Luo, Li Zhi
Publication Title: 
Journal of Economic Entomology

Increasing adoption of transgenic crops expressing cry toxin genes from Bacillus thuringiensis (Bt crops) represents an augmented risk for development of insect resistance. While fitness costs can greatly influence the rate of resistance evolution, most available data related to Bt resistance have been obtained from laboratory-selected insect strains. In this article, we test the existence of fitness costs associated with high levels of field-evolved resistance to Bt maize event TC1507 in a strain of Spodoptera frugiperda (JE Smith) originated from maize fields in Puerto Rico.

Author(s): 
Jakka, S. R. K.
Knight, V. R.
Jurat-Fuentes, J. L.
Publication Title: 
Planta Medica

The Mexican species GALPHIMIA GLAUCA (Cav.) Kuntze (Malphigiaceae) synthesises a family of sedative and anxiolytic nor-secofriedelanes, designated as galphimines. These active principles accumulate at low concentration in the aerial parts of plants from wild populations. Transformed calluses and cell suspension cultures of this species were established in order to induce a greater production of nor-friedelanes. The cell suspension line GgBa was selected and grown over a period of two years of continuous subculturing in MS nutrient medium in the absence of growth regulators.

Author(s): 
Ortíz, Anabel
Cardoso-Taketa, Alexandre
Monroy, Mario Rodríguez
Arellano, Jesús
Hernández, Georgina
Villarreal, María Luisa
Publication Title: 
PloS One

Endophytic actinobacteria colonize internal tissues of their host plants and are considered as a rich and reliable source of diverse species and functional microorganisms. In this study, endophytic actinobacterial strain YIM 63111 was isolated from surface-sterilized tissue of the medicinal plant Artemisia annua. We identified strain YIM 63111 as a member of the genus Pseudonocardia. A. annua seedlings grown under both sterile and greenhouse conditions were inoculated with strain YIM 63111. The growth of A.

Author(s): 
Li, Jie
Zhao, Guo-Zhen
Varma, Ajit
Qin, Sheng
Xiong, Zhi
Huang, Hai-Yu
Zhu, Wen-Yong
Zhao, Li-Xing
Xu, Li-Hua
Zhang, Si
Li, Wen-Jun
Publication Title: 
The Journal of Biological Chemistry

Soluble, truncated mutant and wild-type forms of penicillin-binding protein 5 (sPBP 5) from Escherichia coli were produced in large amounts by placing the dacA gene that encodes PBP 5 under the control of the trp-lac fusion promoter. The 3' end of the dacA gene used in this study contains a stop codon that results in the deletion of 15 amino acids from the carboxyl terminus and the production of a soluble protein. Using oligonucleotide-directed mutagenesis, the role of cysteine 115 in the mechanism of sPBP 5 was investigated.

Author(s): 
Nicholas, R. A.
Strominger, J. L.

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