Biological Assay

Publication Title: 
Homeopathy: The Journal of the Faculty of Homeopathy

We investigated whether water has a 'memory' for succussion compared to unsuccussed controls. The method is based on a bioassay using dinoflagellates. The duration of memory measured by this method is at least 10 min, and may be longer. The effect may be based upon solitons. A hypothesis about the mode of action of homeopathy may be experimentally investigated by this method.

Author(s): 
Tschulakow, A. V.
Yan, Y.
Klimek, W.
Publication Title: 
Homeopathy: The Journal of the Faculty of Homeopathy

OBJECTIVES: The objective was to evaluate homeopathic basic research studies that use plant-based bioassays. With this in view, a compilation was made of the findings of three systematic literature reviews covering plant-based bioassays in the three fields of healthy, abiotically, or biotically stressed plants. This compilation focused on investigations using advanced experimental methods and detailed descriptions, also with the aim of supporting the design of future experiments.

Author(s): 
Jäger, Tim
Scherr, Claudia
Shah, Devika
Majewsky, Vera
Wolf, Ursula
Betti, Lucietta
Baumgartner, Stephan
Publication Title: 
PloS One

Iron is essential for organisms. It is mainly utilized in mitochondria for biosynthesis of iron-sulfur clusters, hemes and other cofactors. Mitoferrin 1 and mitoferrin 2, two homologues proteins belonging to the mitochondrial solute carrier family, are required for iron delivery into mitochondria. Mitoferrin 1 is highly expressed in developing erythrocytes which consume a large amount of iron during hemoglobinization. Mitoferrin 2 is ubiquitously expressed, whose functions are less known.

Author(s): 
Ren, Yaguang
Yang, Su
Tan, Guoqiang
Ye, Wei
Liu, Danhui
Qian, Xu
Ding, Zhongying
Zhong, Yuhong
Zhang, Jingrui
Jiang, Dandan
Zhao, Yuhong
Lu, Jianxin
Publication Title: 
Journal of Medical Entomology

Using the in vitro rearing system in conjunction with the hair tuft bioassay, NYDA and NYDA without fragrances formulations (92% wt:wt dimeticones) were 100% ovicidal (0% of treated eggs hatched) after an 8-h exposure of the eggs of the human head louse (Pediculus humanus capitis De Geer) following the manufacturer's instructions. Comparatively, 78 and 66% of eggs similarly exposed hatched after distilled deionized water or Nix (1% permethrin) treatments, respectively.

Author(s): 
Strycharz, Joseph P.
Lao, Alice R.
Alves, Anna-Maria
Clark, J. Marshall
Publication Title: 
Trends in Pharmacological Sciences

Health problems are rising worldwide, be it as a consequence of lifestyle and longevity in increasingly affluent societies or due to a sharp rise in bacterial antibiotic resistance. The pharmaceutical industry is caught between high rates of attrition and the rather slow pace of a historically large regulatory system for pharmacological safety. Meanwhile, the past decade has seen a tremendous evolution of the biological toolbox, most notably of cellular assays, stem-cell differentiation and organ-mimicking systems. These systems were readily adapted for lead-compound identification.

Author(s): 
Tralau, Tewes
Luch, Andreas
Publication Title: 
Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society

It is well documented that reactive oxygen species (ROS) are involved in the aetiology of age related diseases. Over the last decades, strong efforts have been made to identify antioxidants in human foods and numerous promising compounds have been detected which are used for the production of supplements and functional foods. The present paper describes the advantages and limitations of methods which are currently used for the identification of antioxidants. Numerous in vitro methods are available which are easy to perform and largely used in screening trials.

Author(s): 
Hoelzl, C.
Bichler, J.
Ferk, F.
Simic, T.
Nersesyan, A.
Elbling, L.
Ehrlich, V.
Chakraborty, A.
Knasm¸ller, S.
Publication Title: 
Toxicon: Official Journal of the International Society on Toxinology

American Elapid snakes (Coral Snakes) comprise the genera Leptomicrurus, Micruroides and Micrurus, which form a vast taxonomic assembly of 330 species distributed from the South of United States to the southern region of South America. In order to obtain venom for animal immunizations aimed at antivenom production, Coral Snakes must be kept in captivity and submitted periodically to venom extraction procedures. Thus, to maintain a snake colony in good health for this purpose, a complete alternative diet utilizing an easily obtained prey animal is desirable.

Author(s): 
ChacÛn, Danilo
RodrÌguez, Santos
Arias, JazmÌn
Solano, Gabriela
Bonilla, Fabi·n
GÛmez, AarÛn
Publication Title: 
British Journal of Clinical Pharmacology

1. The pharmacokinetic and effect kinetic properties of oral (p.o.), intramuscular (i.m.), and intrarectal (i.r.) artemether (5 mg kg-1) were compared in a crossover study in eight healthy adult volunteers. Plasma concentrations of artemether (AM) and its active metabolite dihydroartemisinin (DHA) were measured by high performance liquid chromatography with reductive mode electrochemical detection (h.p.l.c.-ECD), and plasma antimalarial activity in vitro (effect) was assessed on the same samples by a sensitive bioassay (BA). 2.

Author(s): 
Teja-Isavadharm, P.
Nosten, F.
Kyle, D. E.
Luxemburger, C.
ter Kuile, F.
Peggins, J. O.
Brewer, T. G.
White, N. J.
Publication Title: 
Antimicrobial Agents and Chemotherapy

The antimalarial activity of artemether following oral or intramuscular administration in the plasma of 15 adults with acute uncomplicated Plasmodium falciparum malaria was measured by bioassay.

Author(s): 
Silamut, Kamolrat
Newton, Paul N.
Teja-Isavadharm, Paktiya
Suputtamongkol, Yupin
Siriyanonda, Duangsuda
Rasameesoraj, Maneerat
Pukrittayakamee, Sasithon
White, Nicholas J.
Publication Title: 
Antimicrobial Agents and Chemotherapy

Artemisinin and its derivatives, artesunate and artemether, are rapidly acting antimalarials that are used for the treatment of severe and uncomplicated multidrug-resistant falciparum malaria. To optimize treatment regimens that use this new class of antimalarials, there is a need for readily available and reproducible assays to monitor drug levels closely in patients. A sensitive and reproducible bioassay for the measurement of the concentrations of artemisinin derivatives in plasma and serum is described.

Author(s): 
Teja-Isavadharm, Paktiya
Peggins, James O.
Brewer, Thomas G.
White, Nicholas J.
Webster, H. Kyle
Kyle, Dennis E.

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