Carbon Isotopes

Publication Title: 
Archives of Biochemistry and Biophysics
Author(s): 
Blackburn, E. H.
Hird, F. J.
Jones, I. K.
Publication Title: 
The Journal of Pharmacology and Experimental Therapeutics
Author(s): 
Peters, M. A.
Publication Title: 
Metabolic Engineering

A key consideration in metabolic engineering is the determination of fluxes of the metabolites within the cell. This determination provides an unambiguous description of metabolism before and/or after engineering interventions. Here, we present a computational framework that combines a constraint-based modeling framework with isotopic label tracing on a large scale.

Author(s): 
Suthers, Patrick F.
Burgard, Anthony P.
Dasika, Madhukar S.
Nowroozi, Farnaz
Van Dien, Stephen
Keasling, Jay D.
Maranas, Costas D.
Publication Title: 
Epilepsia

PURPOSE: Because of the large and continuous energetic requirements of brain function, neurometabolic dysfunction is a key pathophysiologic aspect of the epileptic brain. Additionally, neurometabolic dysfunction has many self-propagating features that are typical of epileptogenic processes, that is, where each occurrence makes the likelihood of further mitochondrial and energetic injury more probable. Thus abnormal neurometabolism may be not only a chronic accompaniment of the epileptic brain, but also a direct contributor to epileptogenesis.

Author(s): 
Pan, Jullie W.
Williamson, Anne
Cavus, Idil
Hetherington, Hoby P.
Zaveri, Hitten
Petroff, Ognen A. C.
Spencer, Dennis D.
Publication Title: 
Journal of Renal Nutrition: The Official Journal of the Council on Renal Nutrition of the National Kidney Foundation

OBJECTIVE: We examined the protein anabolic effects of Pro-Stat 64, a high nitrogen-containing, enzyme-hydrolyzed, tryptophan-fortified, collagen protein supplement administrated during hemodialysis, at two different dosing regimens. DESIGN: This was a randomized, controlled, prospective study with 3 different groups: control, single dose of supplementation, and double dose of supplementation. SETTING: This study was performed at a clinical research center. PATIENTS: Six prevalent chronic hemodialysis (HD) patients were enrolled: 5 males, 1 female, 4 African Americans, and 2 Caucasians.

Author(s): 
Sundell, Mary B.
Cavanaugh, Kerri L.
Wu, Pingsheng
Shintani, Ayumi
Hakim, Raymond M.
Ikizler, T. Alp
Publication Title: 
Pain

Studies in healthy human subjects and patients with irritable bowel syndrome suggest sex differences in cerebral nociceptive processing. Here we examine sex differences in functional brain activation in the rat during colorectal distention (CRD), a preclinical model of acute visceral pain. [(14)C]-iodoantipyrine was injected intravenously in awake, non-restrained female rats during 60- or 0-mmHg CRD while electromyographic abdominal activity (EMG) and pain behavior were recorded.

Author(s): 
Wang, Zhuo
Guo, Yumei
Bradesi, Sylvie
Labus, Jennifer S.
Maarek, Jean-Michel I.
Lee, Kevin
Winchester, Wendy J.
Mayer, Emeran A.
Holschneider, Daniel P.
Publication Title: 
Journal of Agricultural and Food Chemistry

Isotopically labeled tomato carotenoids, phytoene, phytofluene, and lycopene, are needed for mammalian bioavailability and metabolism research but are currently commercially unavailable. The goals of this work were to establish and screen multiple in vitro tomato cell lines for carotenoid production, test the best producers with or without the bleaching herbicides, norflurazon and 2-(4-chlorophenyl-thio)triethylamine (CPTA), and to use the greatest carotenoid accumulator for in vitro 13C-labeling.

Author(s): 
Engelmann, Nancy J.
Campbell, Jessica K.
Rogers, Randy B.
Rupassara, S. Indumathie
Garlick, Peter J.
Lila, Mary Ann
Erdman, John W.
Publication Title: 
Journal of Agricultural and Food Chemistry

Consumption of tomato products has been associated with decreased risks of chronic diseases such as cardiovascular disease and cancer, and therefore the biological functions of tomato carotenoids such as lycopene, phytoene, and phytofluene are being investigated. To study the absorption, distribution, metabolism, and excretion of these carotenoids, a bioengineered Escherichia coli model was evaluated for laboratory-scale production of stable isotope-labeled carotenoids.

Author(s): 
Lu, Chi-Hua
Choi, Jin-Ho
Engelmann Moran, Nancy
Jin, Yong-Su
Erdman, John W.
Publication Title: 
Journal of Agricultural and Food Chemistry

Two different strategies for investigating the likely fate, after ingestion, of natural, bioactive berry constituents (anthocyanins and other non-nutritive flavonoids) are compared. A model of the human gastrointestinal tract (TIM-1) that mimicked the biological environment from the point of swallowing and ingestion through the duodenum, jejunum, and ileum (but not the colon) was used to monitor the stability and bioaccessibility of anthocyanins from both maqui berry and wild blueberry.

Author(s): 
Lila, Mary Ann
Ribnicky, David M.
Rojo, Leonel E.
Rojas-Silva, Patricio
Oren, Andrew
Havenaar, Robert
Janle, Elsa M.
Raskin, Ilya
Yousef, Gad G.
Grace, Mary H.
Publication Title: 
Food Chemistry

While putative disease-preventing lycopene metabolites are found in both tomato (Solanum lycopersicum) products and in their consumers, mammalian lycopene metabolism is poorly understood. Advances in tomato cell culturing techniques offer an economical tool for generation of highly-enriched (13)C-lycopene for human bioavailability and metabolism studies.

Author(s): 
Moran, Nancy Engelmann
Rogers, Randy B.
Lu, Chi-Hua
Conlon, Lauren E.
Lila, Mary Ann
Clinton, Steven K.
Erdman, John W.

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