Carrier Proteins

Publication Title: 
Analytica Chimica Acta

Plumbagin (PL; 5-hydroxy-2-methyl-1,4-naphthoquinone) is a natural compound mainly isolated from Plumbago zeylanica. This plant is distributed in Southeast Asia, and well known as Ayurvedic medicine in India for its medicinal properties. PL has been shown to have various pharmacological activities. We have successfully prepared monoclonal antibodies against PL, and developed an enzyme-linked immunosorbent assay (ELISA) system for determination of PL.

Sakamoto, Seiichi
Putalun, Waraporn
Tsuchihashi, Ryota
Morimoto, Satoshi
Kinjo, Junei
Tanaka, Hiroyuki
Publication Title: 
Journal of Ethnopharmacology

AIM OF THIS STUDY: Cardiospermum halicacabum L. is well known for its anti-inflammatory, analgesic and antipyretic activities. It has been used in Ayurveda and folk medicine for the treatment of rheumatism, fever and earache. But its mechanism of anti-inflammatory and analgesic action is still unclear, hence in this context, the objective of our study is to reveal the mechanism of anti-inflammatory and analgesic activity of Cardiospermum halicacabum L. which would form an additional proof to the traditional knowledge of Cardiospermum halicacabum L.

Sheeba, M. S.
Asha, V. V.
Publication Title: 
The Journal of Biological Chemistry

Soluble, truncated mutant and wild-type forms of penicillin-binding protein 5 (sPBP 5) from Escherichia coli were produced in large amounts by placing the dacA gene that encodes PBP 5 under the control of the trp-lac fusion promoter. The 3' end of the dacA gene used in this study contains a stop codon that results in the deletion of 15 amino acids from the carboxyl terminus and the production of a soluble protein. Using oligonucleotide-directed mutagenesis, the role of cysteine 115 in the mechanism of sPBP 5 was investigated.

Nicholas, R. A.
Strominger, J. L.
Publication Title: 
Science (New York, N.Y.)

Multiple death signals influence mitochondria during apoptosis, yet the critical initiating event for mitochondrial dysfunction in vivo has been unclear. tBID, the caspase-activated form of a "BH3-domain-only" BCL-2 family member, triggers the homooligomerization of "multidomain" conserved proapoptotic family members BAK or BAX, resulting in the release of cytochrome c from mitochondria. We find that cells lacking both Bax and Bak, but not cells lacking only one of these components, are completely resistant to tBID-induced cytochrome c release and apoptosis.

Wei, M. C.
Zong, W. X.
Cheng, E. H.
Lindsten, T.
Panoutsakopoulou, V.
Ross, A. J.
Roth, K. A.
MacGregor, G. R.
Thompson, C. B.
Korsmeyer, S. J.
Publication Title: 

BACKGROUND: Developmentally regulated endothelial locus-1 (Del-1) is an extracellular matrix protein that is expressed by endothelial cells during embryological vascular development. We speculated that Del-1 may be reexpressed in ischemia and may be involved in endogenous angiogenesis. METHODS AND RESULTS: Del-1 protein was detected by immunohistochemistry in murine ischemic hindlimb after femoral artery excision. To determine whether exogenous Del-1 would augment angiogenesis in vivo, Del-1 or vehicle was administered for 3 weeks by intramuscular injection of murine ischemic hindlimbs.

Ho, Hoai-Ky V.
Jang, James J.
Kaji, Shuichiro
Spektor, Gary
Fong, Annie
Yang, Phillip
Hu, Bob S.
Schatzman, Randy
Quertermous, Thomas
Cooke, John P.
Publication Title: 
Experimental Cell Research

Eukaryote cells balance production of reactive oxygen species (ROS) with levels of anti-oxidant enzyme activity to maintain cellular redox homeostasis. Mitochondria are a major source of ROS, while many anti-oxidant genes are regulated by the Nrf2 transcription factor. Keap1, a redox-regulated substrate adaptor for a cullin-based ubiquitin ligase, targets Nrf2 for proteosome-mediated degradation and represses Nrf2-dependent gene expression. We have previously identified a member of the phosphoglycerate mutase family, PGAM5, as a Keap1-binding protein.

Lo, Shih-Ching
Hannink, Mark
Publication Title: 
Human Molecular Genetics

Variants of a hexanucleotide repeat polymorphism in the promoter of the 5-lipoxygenase (5-LO) gene have been associated with cardiovascular disease traits in humans, which may be due, at least in part, to differential expression of the at-risk alleles. To more fully characterize these variants, we carried out gene expression and DNA methylation studies in primary leukocytes from healthy individuals carrying various 5-LO promoter alleles.

Vikman, Susanna
Brena, Romulo M.
Armstrong, Patrice
Hartiala, Jaana
Stephensen, Charles B.
Allayee, Hooman
Publication Title: 
Brain: A Journal of Neurology

Distal myopathies are a heterogeneous group of disorders characterized by progressive weakness and muscular atrophy, beginning in distal limb muscles and affecting proximal limb muscles at a later stage. We studied a large German kindred with 10 affected members. Weakness and atrophy of the anterior tibial muscles started between the ages of 8 and 16 years, followed by atrophy of intrinsic hand muscles. Progression was slow, and patients retained the ability to walk until the seventh decade. Serum creatinine kinase levels were increased in the range of 150-1400 U/l.

Cirak, Sebahattin
von Deimling, Florian
Sachdev, Shrikesh
Errington, Wesley J.
Herrmann, Ralf
Bönnemann, Carsten
Brockmann, Knut
Hinderlich, Stephan
Lindner, Tom H.
Steinbrecher, Alice
Hoffmann, Katrin
Privé, Gilbert G.
Hannink, Mark
Nürnberg, Peter
Voit, Thomas
Publication Title: 
The Journal of Nutrition

Chromium was proposed to be an essential element over 50 y ago and was shown to have therapeutic potential in treating the symptoms of type 2 diabetes; however, its mechanism of action at a molecular level is unknown. One chromium-binding biomolecule, low-molecular weight chromium-binding substance (LMWCr or chromodulin), has been found to be biologically active in in vitro assays and proposed as a potential candidate for the in vivo biologically active form of chromium. Characterization of the organic component of LMWCr has proven difficult.

Chen, Yuan
Watson, Heather M.
Gao, Junjie
Sinha, Sarmistha Halder
Cassady, Carolyn J.
Vincent, John B.
Publication Title: 
The Plant Cell

Plant phototropism is an adaptive response to changes in light direction, quantity, and quality that results in optimization of photosynthetic light harvesting, as well as water and nutrient acquisition. Though several components of the phototropic signal response pathway have been identified in recent years, including the blue light (BL) receptors phototropin1 (phot1) and phot2, much remains unknown. Here, we show that the phot1-interacting protein NONPHOTOTROPIC HYPOCOTYL3 (NPH3) functions as a substrate adapter in a CULLIN3-based E3 ubiquitin ligase, CRL3(NPH3).

Roberts, Diana
Pedmale, Ullas V.
Morrow, Johanna
Sachdev, Shrikesh
Lechner, Esther
Tang, Xiaobo
Zheng, Ning
Hannink, Mark
Genschik, Pascal
Liscum, Emmanuel


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