Caspase 3

Publication Title: 
Molecular Cancer Therapeutics

The present study was undertaken to gain insights into the molecular mechanism of cell death (apoptosis) by guggulsterone, a constituent of Ayurvedic medicinal plant Commiphora mukul, using PC-3 human prostate cancer cells as a model. The viability of PC-3 cells, but not a normal prostate epithelial cell line (PrEC), was reduced significantly on treatment with guggulsterone in a concentration-dependent manner.

Author(s): 
Singh, Shivendra V.
Zeng, Yan
Xiao, Dong
Vogel, Victor G.
Nelson, Joel B.
Dhir, Rajiv
Tripathi, Yamini B.
Publication Title: 
PloS One

The medicinal plant Withania somnifera has been used for over centuries in Indian Ayurvedic Medicine to treat a wide spectrum of disorders. Withaferin A (WA), a bioactive compound that is isolated from this plant, has anti-inflammatory, immuno-modulatory, anti-angiogenic, and anti-cancer properties. Here we investigated malignant pleural mesothelioma (MPM) suppressive effects of WA and the molecular mechanisms involved.

Author(s): 
Yang, Huanjie
Wang, Ying
Cheryan, Vino T.
Wu, Wenjuan
Cui, Cindy Qiuzhi
Polin, Lisa A.
Pass, Harvey I.
Dou, Q. Ping
Rishi, Arun K.
Wali, Anil
Publication Title: 
Molecular Cancer Therapeutics

The present study was undertaken to gain insights into the molecular mechanism of cell death (apoptosis) by guggulsterone, a constituent of Ayurvedic medicinal plant Commiphora mukul, using PC-3 human prostate cancer cells as a model. The viability of PC-3 cells, but not a normal prostate epithelial cell line (PrEC), was reduced significantly on treatment with guggulsterone in a concentration-dependent manner.

Author(s): 
Singh, Shivendra V.
Zeng, Yan
Xiao, Dong
Vogel, Victor G.
Nelson, Joel B.
Dhir, Rajiv
Tripathi, Yamini B.
Publication Title: 
Cancer Letters

Swertia chirata, is a bitter plant, used in the Indian system of medicine (Ayurveda) for various human ailments. Our laboratory was the first to report the chemopreventive effect of this plant. The antiproliferative and pro-apoptotic action of amarogentin rich fraction of S. chirata is now demonstrated on a mouse skin carcinogenesis model. Immunohistochemical localization revealed a reduction in proliferating and increase in apoptotic cells in skin lesion following treatment, also reflected in the expression of molecular markers--Cox-II and caspase-3 proteins.

Author(s): 
Saha, Prosenjit
Mandal, Suvra
Das, Ashes
Das, Sukta
Publication Title: 
Breast Cancer Research and Treatment

25-Hydroxy-3-oxoolean-12-en-28-oic acid (Amooranin-AMR) is a triterpene acid isolated from the stem bark of a tropical tree (Amoora rohituka) grown wild in India. A herbal preparation used for the treatment of cancer by the Ayurvedic system of medicine contains the stem bark of Amoora rohituka as one of the ingredients. In this paper, we show that AMR displays a strong inhibitory effect on survival of human breast carcinoma MDA-468, breast adenocarcinoma MCF-7 cells compared to breast epithelial MCF-10A control cells.

Author(s): 
Rabi, Thangaiyan
Wang, Liming
Banerjee, Sipra
Publication Title: 
Journal of Ethnopharmacology

ETHNOPHARMACOLOGICAL RELEVANCE: Achyranthes aspera (Family Amaranthacea) is used for cancer therapy by ayurvedic medical practitioners in India. However, due to the non formal nature of its use, there are no systematic studies validating its medicinal properties. Thus, it's utility as an anti cancer agent remains anecdotal. Earlier, we demonstrated A. aspera to exhibit time and dose-dependent preferential cytotoxicity to cultured human pancreatic cancer cells. In this report we validate in vivo anti tumor properties of A. aspera.

Author(s): 
Subbarayan, Pochi R.
Sarkar, Malancha
Nagaraja Rao, Shamaladevi
Philip, Sakhi
Kumar, Pradeep
Altman, Norman
Reis, Isildinha
Ahmed, Mansoor
Ardalan, Bach
Lokeshwar, Bal L.
Publication Title: 
PloS One

The medicinal plant Withania somnifera has been used for over centuries in Indian Ayurvedic Medicine to treat a wide spectrum of disorders. Withaferin A (WA), a bioactive compound that is isolated from this plant, has anti-inflammatory, immuno-modulatory, anti-angiogenic, and anti-cancer properties. Here we investigated malignant pleural mesothelioma (MPM) suppressive effects of WA and the molecular mechanisms involved.

Author(s): 
Yang, Huanjie
Wang, Ying
Cheryan, Vino T.
Wu, Wenjuan
Cui, Cindy Qiuzhi
Polin, Lisa A.
Pass, Harvey I.
Dou, Q. Ping
Rishi, Arun K.
Wali, Anil
Publication Title: 
Cell Proliferation

OBJECTIVES: Gymnema montanum Hook, an Indian Ayurvedic medicinal plant, is used traditionally to treat a variety of ailments. Here, we report anti-cancer effects and molecular mechanisms of ethanolic extract of G. montanum (GLEt) on human leukaemia HL-60 cells, compared to peripheral blood mononuclear cells. MATERIALS AND METHODS: HL-60 cells were treated with different concentrations of GLEt (10-50 μg/ml) and cytotoxicity was assessed by MTT assay. Levels of lipid peroxidation, antioxidants, mitochondrial membrane potential and caspase-3 were measured.

Author(s): 
Ramkumar, K. M.
Manjula, C.
Elango, B.
Krishnamurthi, K.
Saravana Devi, S.
Rajaguru, P.
Publication Title: 
Proceedings of the National Academy of Sciences of the United States of America

Standardized extract from the leaves of the Ginkgo biloba tree, labeled EGb761, has been used in clinical trials for its beneficial effects on brain functions, particularly in connection with age-related dementias and Alzheimer's disease (AD). Substantial experimental evidence indicates that EGb761 protects against neuronal damage from a variety of insults, but its cellular and molecular mechanisms remain unknown.

Author(s): 
Luo, Yuan
Smith, Julie V.
Paramasivam, Vijaykumar
Burdick, Adam
Curry, Kenneth J.
Buford, Justin P.
Khan, Ikhlas
Netzer, William J.
Xu, Huaxi
Butko, Peter
Publication Title: 
The Journal of Biological Chemistry

Identification of the polyamine transporter gene will be useful for modulating polyamine accumulation in cells and should be a good target for controlling cell proliferation. Polyamine transport activity in mammalian cells is critical for accumulation of the polyamine analog methylglyoxal bis(guanylhydrazone) (MGBG) that induces apoptosis, although a gene responsible for transport activity has not been identified. Using a retroviral gene trap screen, we generated MGBG-resistant Chinese hamster ovary (CHO) cells to identify genes involved in polyamine transport activity.

Author(s): 
Fukuchi, Junichi
Hiipakka, Richard A.
Kokontis, John M.
Nishimura, Kazuhiro
Igarashi, Kazuei
Liao, Shutsung

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