Caspase 3

Publication Title: 
Cancer Research

Acetyl-keto-beta-boswellic acid (AKBA), a triterpenoid isolated from Boswellia carterri Birdw and Boswellia serrata, has been found to inhibit tumor cell growth and to induce apoptosis. The apoptotic effects and the mechanisms of action of AKBA were studied in LNCaP and PC-3 human prostate cancer cells. AKBA induced apoptosis in both cell lines at concentrations above 10 microg/mL. AKBA-induced apoptosis was correlated with the activation of caspase-3 and caspase-8 as well as with poly(ADP)ribose polymerase (PARP) cleavage.

Author(s): 
Lu, Min
Xia, Lijuan
Hua, Huiming
Jing, Yongkui
Publication Title: 
Cancer Research

We have identified a natural compound that activates apoptosis of epithelial cancer cells through activation of tumor necrosis factor-alpha (TNF-alpha), TNF receptor (TNFR)-associated death domain (TRADD), and caspases. The molecule 1-hydroxy-5,7-dimethoxy-2-naphthalene-carboxaldehyde (HDNC, marmelin) was isolated and characterized from ethyl acetate fraction of extracts of Aegle marmelos. HDNC treatment inhibited the growth of HCT-116 colon cancer tumor xenografts in vivo.

Author(s): 
Subramaniam, Dharmalingam
Giridharan, Periyasamy
Murmu, Nabendu
Shankaranarayanan, Nallakandy P.
May, Randal
Houchen, Courtney W.
Ramanujam, Rama P.
Balakrishnan, Arun
Vishwakarma, Ram A.
Anant, Shrikant
Publication Title: 
Apoptosis: An International Journal on Programmed Cell Death

Isoflavone genistein may have beneficial effects on vascular function, but the mechanism is unclear. Here, we investigated whether genistein protects vascular endothelial cells against apoptosis induced by tumor necrosis factor-alpha. We show that genistein significantly inhibited TNF-alpha-induced apoptosis in human aortic endothelial cells as determined by caspase-3 activation, 7-amino actinomycin D staining, in situ apoptotic cell detection and DNA laddering. The anti-apoptotic effect of genistein was associated with an enhanced expression of Bcl-2 protein and its promoter activity.

Author(s): 
Si, Hongwei
Liu, Dongmin
Publication Title: 
Endocrinology

Beta-cell apoptosis occurs in diabetes mellitus (DM). Heat shock protein (HSP) 27 (human homolog of rodent HSP25) mitigates stress-induced apoptosis but has not been studied in beta-cells. We tested whether HSP27 overexpression attenuates streptozotocin (SZ)-induced DM in vivo and cytokine-induced islet apoptosis in vitro. DM was ascertained by ip glucose tolerance testing, and fasting serum insulin/glucose was measured. Pancreas was stained for insulin, HSP27, and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling, and insulin content was measured.

Author(s): 
Dai, Tiane
Patel-Chamberlin, Mina
Natarajan, Rama
Todorov, Ivan
Ma, Jun
LaPage, Janine
Phillips, Lynetta
Nast, Cynthia C.
Becerra, Diana
Chuang, Peter
Tong, Lili
de Belleroche, Jacqueline
Wells, Dominic J.
Wang, Ying
Adler, Sharon G.
Publication Title: 
Arthritis and Rheumatism

OBJECTIVE: Overexpression of the antiapoptotic protein myeloid cell leukemia 1 (Mcl-1) in rheumatoid arthritis (RA) synovial fibroblasts is a major cause of their resistance to tumor necrosis factor alpha (TNFalpha)-induced apoptosis. This study was undertaken to evaluate the efficacy of epigallocatechin-3-gallate (EGCG) in down-regulating Mcl-1 expression and its mechanism of RA synovial fibroblast sensitization to TNFalpha-induced apoptosis.

Author(s): 
Ahmed, Salahuddin
Silverman, Matthew D.
Marotte, Hubert
Kwan, Kevin
Matuszczak, Natalie
Koch, Alisa E.
Publication Title: 
BMC biology

BACKGROUND: We have recently shown that delta-opioid receptors (DORs) play an important role in neuroprotection from hypoxic injury via the regulation of extracellular signaling-regulated kinase (ERK) and cytochrome c release. Since ERK and cytochrome c are differentially involved in caspase signaling of oxidative injury that significantly contributes to neuronal damage in ischemia/reperfusion, we considered if DOR activation protects the ischemic brain by attenuating oxidative injury.

Author(s): 
Yang, Yilin
Xia, Xiwei
Zhang, Yi
Wang, Qiang
Li, Lu
Luo, Guanghua
Xia, Ying
Publication Title: 
Journal of Experimental Therapeutics & Oncology

Resveratrol (trans-3,4', 5-trihydroxystilbene) is a naturally occurring polyphenolic compound that has antiinflammatory, antioxidant, neuroprotective properties and acts as a chemopreventive agent. Resveratrol causes cell cycle arrest and induces apoptotic cell death in various types of cancer cells. In the current studies, the effect of resveratrol on phosphoinositide kinase-3 (PI3K)/protein kinase B (Akt)/mammalian target of rapamycin (mTOR) signaling pathway was examined in human U251 glioma cells. Resveratrol decreased both the expression and phosphorylation of Akt.

Author(s): 
Jiang, Hao
Shang, Xia
Wu, Hongtao
Gautam, Subhash C.
Al-Holou, Shaza
Li, Christina
Kuo, Jarret
Zhang, Lijie
Chopp, Michael
Publication Title: 
Nutrition and Cancer

Stearic acid (stearate) is an 18-carbon saturated fatty acid that has been shown to inhibit invasion and proliferation and induce apoptosis in various human cell types. The specificity of stearate-induced apoptosis for cancerous versus noncancerous breast cells has not been examined, and the mechanism underlying stearate-induced apoptosis is unknown. Morphological analysis, cell viability, and caspase-3 activity assays demonstrated that stearate activated apoptosis preferentially in cancerous breast cells in a time- and dose-dependent manner.

Author(s): 
Evans, Lynda M.
Cowey, Stephanie L.
Siegal, Gene P.
Hardy, Robert W.
Publication Title: 
American Journal of Physiology. Gastrointestinal and Liver Physiology

The inflammatory response during pancreatitis regulates necrotic and apoptotic rates of parenchymal cells. Neutrophil depletion by use of anti-polymorphonuclear serum (anti-PMN) increases apoptosis in experimental pancreatitis but the mechanism has not been determined. Our study was designed to investigate signaling mechanisms in pancreatic parenchymal cells regulating death responses with neutrophil depletion. Rats were neutrophil depleted with anti-PMN treatment. Then cerulein pancreatitis was induced, followed by measurements of apoptosis signaling pathways.

Author(s): 
Nakamura, Yuji
Do, Jae Hyuk
Yuan, Jingzhen
Odinokova, Irina V.
Mareninova, Olga
Gukovskaya, Anna S.
Pandol, Stephen J.
Publication Title: 
The Journal of Pharmacy and Pharmacology

OBJECTIVES: Panaxadiol is a purified sapogenin of ginseng saponins that exhibits anticancer activity. Irinotecan is a second-line anticancer drug, but clinical treatment with irinotecan is limited due to its side effects. In this study, we have investigated the possible synergistic anticancer effects of panaxadiol and irinotecan on human colorectal cancer cells and explored the potential role of apoptosis in their synergistic activity. KEY FINDINGS: The combination of panaxadiol and irinotecan significantly enhanced antiproliferative effects in HCT-116 cells (P< 0.05).

Author(s): 
Du, Guang-Jian
Wang, Chong-Zhi
Zhang, Zhi-Yu
Wen, Xiao-Dong
Somogyi, Jacqueline
Calway, Tyler
He, Tong-Chuan
Du, Wei
Yuan, Chun-Su

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