Caspase 3

Publication Title: 
Zhong Xi Yi Jie He Xue Bao = Journal of Chinese Integrative Medicine

OBJECTIVE: To observe the effects of Tenglong Buzhong Decoction (TLBZD), a compound traditional Chinese herbal medicine, on proliferation and apoptosis of colon carcinoma cell line LS174T in vitro. METHODS: Human colon carcinoma cell line LS174T and human colon epithelial cell line CRL-1790 were treated with different doses of TLBZD. Cell proliferation was detected with cell counting kit-8 (CCK-8) assay and clone formation assay. Cell cycle and apoptosis were detected by flow cytometry, and caspase-3, -8 and -9 activities in LS174T cells were detected by colorimetric assay.

Author(s): 
Hu, Bing
An, Hong-Mei
Shen, Ke-Ping
Du, Qin
Publication Title: 
Acta Pharmacologica Sinica

AIM: to examine the effects of tanshinone IIA, the main effective component of Salvia miltiorrhiza (known as 'Danshen' in traditional Chinese medicine) on angiotensin II (Ang II)-mediated cardiomyocyte apoptosis. METHODS: rat neonatal cardiomyocytes were primarily cultured with Ang II or Ang II plus tanshinone IIA. Myocyte apoptosis was evaluated by caspase-3 activity and DNA strand break level with TdT-mediated dUTP nick-end labeling (TUNEL) staining.

Author(s): 
Hong, Hong-Jye
Liu, Ju-Chi
Cheng, Tzu-hurng
Chan, Paul
Publication Title: 
Zhong Xi Yi Jie He Xue Bao = Journal of Chinese Integrative Medicine

OBJECTIVE: The present study investigates the effects of Tanreqing injection, a compound Chinese herbal medicine, on the proliferation of leukemia cells in vitro and discusses the potential mechanisms. METHODS: Tanreqing injection was diluted to a series of concentrations (1:2, 1:4, 1:8, 1:16, 1:32, 1:64, 1:128, 1:256 and 1:512) by volume and then independently applied to treat chronic myeloid leukemia K562 cells and T cell acute lymphocytic leukemia Molt4 cells at the proliferative stage. Cell growth was observed at different time intervals under a microscope.

Author(s): 
Yang, Bo
Lu, Xue-chun
Zhang, Feng
Liu, Yang
Zhu, Hong-li
Publication Title: 
Zhong Xi Yi Jie He Xue Bao = Journal of Chinese Integrative Medicine

OBJECTIVE: To investigate the different efficacy of Yinchenhao Decoction (YCHD), a compound traditional Chinese herbal medicine, for liver cirrhosis induced by dimethylnitrosamine (DMN) or carbon tetrachloride (CCl(4)) in rats. METHODS: To induce liver fibrosis, 0.5% DMN solution (2mL/kg body weight, i.p.) was given three consecutive days a week to male Wistar rats for 4 weeks. Cirrhotic rats were randomly divided into DMN group, YCHD group, Xiaochaihu decoction group by the end of the fourth week to accomplish a 2-week recipe treatment course.

Author(s): 
Sun, Ming-yu
Wang, Lei
Mu, Yong-ping
Liu, Cheng
Bian, Yan-qin
Wang, Xiao-ning
Liu, Ping
Publication Title: 
Asian Pacific journal of cancer prevention: APJCP

BACKGROUND: Study of the mechanisms of apoptosis in tumor cells is an important field of tumor therapy and cancer molecular biology. Apoptosis triggered by activation of the mitochondrial-dependent caspase pathway represents the main programmed cell death mechanism. The mitochondrial-dependent apoptosis pathway is activated by various intracellular stresses that induce permeabilization of the mitochondrial membrane, leading to cytochrome C release.

Author(s): 
Gao, Lin-Lin
Li, Fu-Rong
Jiao, Peng
Yao, Shu-Tong
Sang, Hui
Si, Yan-Hong
Publication Title: 
Molecules (Basel, Switzerland)

Typhonium giganteum Engl. (BaiFuzi) is one of the herbs commonly used in traditional Chinese medicine against cancer. In our previous studies, 37 compounds were identified the SFE-CO(2) (supercritical fluid extraction with CO(2)) extract by GC-MS, including the four major components [β-sitosterol (40.22%), campesterol (18.45%), n-hexadecanoic acid (9.52%) and (Z,Z)-9,12-octadecadienoic acid (8.15%)]. The anti-cancer mechanisms of the SFE-CO(2 )extract from T. giganteum Engl. tubers have not been reported as yet.

Author(s): 
Li, Qingyong
Jiang, Chunfei
Zu, Yuangang
Song, Zhen
Zhang, Baoyou
Meng, Xiangdong
Qiu, Wei
Zhang, Li
Publication Title: 
Molecules (Basel, Switzerland)

Thalictrum fortunei S. Moore, a perennial plant distributed in the southeastern part of China, has been used in Traditional Chinese Medicine for thousands of years for its antitumor, antibacterial and immunoregulatory effects. In order to investigate the active components and the mechanism of the anti-tumor effects of Thalictrum fortunei, the growth inhibitory effects of eight triterpenoids isolated from the aerial parts of the plant on tumor cell lines were examined by 3-(4,5)-dimethylthiazoy1-3,5-diphenyltetrazolium bromide (MTT) assay.

Author(s): 
Zhang, Xiantao
Zhao, Ming
Chen, Lvyi
Jiao, Haoyan
Liu, Hongxia
Wang, Liyan
Ma, Shuwei
Publication Title: 
International Journal of Nanomedicine

Traditional Chinese medicine is gradually becoming a new source of anticancer drugs. One such example is wogonin, which is cytotoxic to various cancer cell lines in vitro. However, due to its low water solubility, wogonin is restricted to clinical administration. Recently, the application of drug-coated magnetic nanoparticles (MNPs) to increase water solubility of the drug and to enhance its chemotherapeutic efficiency has attracted much attention.

Author(s): 
Wang, Lei
Zhang, Haijun
Chen, Baoan
Xia, Guohua
Wang, Shuai
Cheng, Jian
Shao, Zeye
Gao, Chong
Bao, Wen
Tian, Liang
Ren, Yanyan
Xu, Peipei
Cai, Xiaohui
Liu, Ran
Wang, Xuemei
Publication Title: 
Asian Pacific journal of cancer prevention: APJCP

BACKGROUND: The effect and possible mechanism of traditional Chinese medicine, baicalin, on the PI3K/ Akt signaling pathway in drug-resistant human myeloid leukemia HL-60/ADR cells have been investigated in this current study. METHODS: HL-60/ADR cells were treated by 20, 40, 80 μmol/L baicalin followed by cell cycle analysis at 24h. The mRNA expression level of the apoptosis related gene, Bcl-2 and bad, were measured by RT-PCR on cells treated with 80 μmol/L baicalin at 12, 24 and 48hr.

Author(s): 
Zheng, Jing
Hu, Jian-Da
Chen, Ying-Yu
Chen, Bu-Yuan
Huang, Yi
Zheng, Zhi-Hong
Liu, Ting-Bo
Publication Title: 
PloS One

BACKGROUND: Traditional Chinese medicinal herbs Cortex Moutan and Radix Salviae Milthiorrhizaeare are prescribed together for their putative cardioprotective effects in clinical practice. However, the rationale of the combined use remains unclear. The present study was designed to investigate the cardioprotective effects of paeonol and danshensu (representative active ingredient of Cortex Moutan and Radix Salviae Milthiorrhizae, respectively) on isoproterenol-induced myocardial infarction in rats and its underlying mechanisms.

Author(s): 
Li, Hua
Xie, Yan-Hua
Yang, Qian
Wang, Si-Wang
Zhang, Bang-Le
Wang, Jian-Bo
Cao, Wei
Bi, Lin-Lin
Sun, Ji-Yuan
Miao, Shan
Hu, Jing
Zhou, Xuan-Xuan
Qiu, Peng-Cheng

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