Chromatography, Ion Exchange

Publication Title: 
The Journal of Biological Chemistry
Author(s): 
Davidson, B. E.
Blackburn, E. H.
Dopheide, T. A.
Publication Title: 
International Journal of Biological Macromolecules

Asparagus racemosus Linn. (Fam. Liliaceae) is an ethno-pharmacologically acclaimed Ayurvedic medicinal plant. In the present study, aqueous extract of A. racemosus (ARC) was fractionated and screened for the polysaccharide fraction (ARP). The characterization was done by enzymatic, Size Exclusion, gas chromatography with flame ionization detector (GC-FID), high pressure anion exchange chromatography (HPAEC) and thin layer chromatographic analyses. Phyto-chemical evaluation confirmed the presence of 26.7% of 2→1 linked fructo-oligosaccharides (FOS).

Author(s): 
Thakur, Mayank
Connellan, Paul
Deseo, Myrna A.
Morris, Carol
Praznik, Werner
Loeppert, Renate
Dixit, V. K.
Publication Title: 
Journal of Ethnopharmacology

ETHNOPHARMACOLOGICAL RELEVANCE: Guduchi (Tinospora cordifolia), a widely used plant in folk and Ayurvedic systems of medicine is well known for its immunomodulatory activity; however, the presence of an immunomodulatory protein (ImP) in guduchi has not been investigated. MATERIALS AND METHODS: Guduchi ImP was purified from dry stem powder extract by anion-exchange chromatography on Q-Sepharose. Characterization of guduchi ImP was performed by SDS-PAGE, periodic acid-Schiff staining, HPLC, and immunochemical analyses.

Author(s): 
Aranha, Ivan
Clement, Fatima
Venkatesh, Yeldur P.
Publication Title: 
Journal of Chromatography. A

The availability of sufficient quantities of DNA of adequate quality is crucial in polymerase chain reaction (PCR)-based methods for genetically modified food detection. In this work, the suitability of anion-exchange CIM (Convective Interaction Media; BIA Separations, Ljubljana, Slovenia) monolithic columns for isolation of DNA from food was studied. Maize and its derivates corn meal and thermally pretreated corn meal were chosen as model food. Two commercially available CIM disk columns were tested: DEAE (diethylaminoethyl) and QA (quaternary amine).

Author(s): 
Jerman, Sergej
Podgornik, Ales
Cankar, Katarina
Cadet, Neza
Skrt, Mihaela
Zel, Jana
Raspor, Peter
Publication Title: 
Molecules (Basel, Switzerland)

During our investigation of the neuroprotective activity of Platycodi radix we found that an aqueous extract of this folk medicine exhibited significant protection against glutamate-induced toxicity in primary cultured rat cortical cells. In order to clarify the neuroprotective mechanism(s) of this observed effect, activity-guided isolation was performed to seek and identify active fractions and components. By such fractionation, four known triterpene saponin compounds--platycodins A, C and D and deapioplatycodin D--were isolated from the n-butanol fraction.

Author(s): 
Son, Il Hong
Park, Yong Hoon
Lee, Sung Ik
Yang, Hyun Duk
Moon, Hyung-In
Publication Title: 
Acta Biochimica Polonica

Plant latex has many health benefits and has been used in folk medicine. In this study, the biological effect of Artocarpus heterophyllus (jackfruit) latex on human blood coagulation was investigated. By a combination of heat precipitation and ion-exchange chromatography, a heat stable heteromultimeric glycoprotein (HSGPL1) was purified from jackfruit milky latex. The apparent molecular masses of the monomeric proteins on SDS/PAGE were 33, 31 and 29 kDa. The isoelectric points (pIs) of the monomers were 6.63, 6.63 and 6.93, respectively.

Author(s): 
Siritapetawee, Jaruwan
Thammasirirak, Sompong
Publication Title: 
Journal of Food Science

Individuals with phenylketonuria (PKU) cannot metabolize phenylalanine (Phe) and must adhere to a low-Phe diet in which most dietary protein is provided by a Phe-free amino acid formula. Glycomacropeptide (GMP) is the only naturally occurring protein that does not contain Phe, and is of interest as a source of protein for dietary management of PKU. However, commercially available GMP contains too much Phe from residual whey proteins and does not contain adequate levels of all the indispensable amino acids to provide a nutritionally complete protein.

Author(s): 
Laclair, Caitlin E.
Ney, Denise M.
MacLeod, Erin L.
Etzel, Mark R.
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