Chromatography, Thin Layer

Publication Title: 
Journal of Ethnopharmacology

Ethanolic extracts of 45 Indian medicinal plants traditionally used in medicine were studied for their antimicrobial activity against certain drug-resistant bacteria and a yeast Candida albicans of clinical origin. Of these, 40 plant extracts showed varied levels of antimicrobial activity against one or more test bacteria. Anticandidal activity was detected in 24 plant extracts. Overall, broad-spectrum antimicrobial activity was observed in 12 plants (L. inermis, Eucalyptus sp., H. antidysentrica, H. indicus, C. equistifolia. T. belerica, T. chebula, E. officinalis, C. sinensis, S.

Author(s): 
Ahmad, I.
Beg, A. Z.
Publication Title: 
Journal of Chromatography. A

Traditional Chinese Herbal Medicine (TCHM) contain multiple botanicals, each of which contains many compounds that may be relevant to the medicine's putative activity. Therefore, analytical techniques that look at a suite of compounds, including their respective ratios, provide a more rational approach to the authentication and quality assessment of TCHM.

Author(s): 
Xie, Peishan
Chen, Sibao
Liang, Yi-Zeng
Wang, Xianghong
Tian, Runtao
Upton, Roy
Publication Title: 
Journal of Biosciences

Myrrh (guggulu) oleoresin from the Commiphora mukul tree is an important component of antiarthritic drugs in Ayurvedic medicine. Clinical data suggest that elevated levels of hyaluronidase and collagenase type 2 enzymes contribute significantly to cartilage degradation. Triphala guggulu (TG) is a guggulu-based formulation used for the treatment of arthritis. We assessed the chondroprotective potential of TG by examining its effects on the activities of pure hyaluronidase and collagenase type 2 enzymes.

Author(s): 
Sumantran, Venil N.
Kulkarni, Asavari A.
Harsulkar, Abhay
Wele, Asmita
Koppikar, Soumya J.
Chandwaskar, Rucha
Gaire, Vishakha
Dalvi, Madhuri
Wagh, Ulhas V.
Publication Title: 
Pakistan journal of biological sciences: PJBS

The antibacterial activity of acetone, hexane, dichloromethane leaf extract of five Terminalia species (Terminalia alata Heyne ex Roth., Terminalia arjuna (Roxb.) Wt. and Am., Terminalia bellerica (Gaertn.) Roxb., Terminalia catappa L. and Terminalia chebula Retz.) were tested by Agar-well-diffusion method against human pathogens E. coli, Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus and Staphylococcus epidermidis. The Rf values and relative activities of separated compounds were tested.

Author(s): 
Shinde, S. L.
Junne, S. B.
Wadje, S. S.
Baig, M. M. V.
Publication Title: 
Phytotherapy research: PTR

The hydroalcoholic extract of fruit pulp of Terminalia chebula Retz. was standardized and evaluated for its safety through cytochrome P450 (CYP 450) inhibition assay. Standardization was performed through high performance thin layer chromatography (HPTLC) using gallic acid (GA) standard. Cytochrome P450-CO complex microplate assay was performed using rat liver microsomes. The effect of standardized extract, its fraction and bioactive marker compound were comparatively evaluated for its effect on CYP P450 enzymes.

Author(s): 
Ponnusankar, S.
Pandit, S.
Venkatesh, M.
Bandyopadhyay, A.
Mukherjee, Pulok K.
Publication Title: 
International Journal of Cosmetic Science

Lipases play an important role in pathogenesis of acne by hydrolysing sebum triglycerides and releasing irritating free fatty acids in the pilosebaceous follicles. Lipase is a strong chemotactic and proinflammatory antigen. Therefore, lipase has generated a high interest as a pharmacological target for antiacne drugs. The aim of this study was to identify inhibitory effects of plant extracts on the lipase activity of Propionibacterium acnes. Colorimetric microassay was used to determine lipase activity.

Author(s): 
Patil, V.
Bandivadekar, A.
Debjani, D.
Publication Title: 
Zhongguo Yao Li Xue Bao = Acta Pharmacologica Sinica

TLC scanning technique was found to have good specificity for studying the absorption and distribution of artemether in rats. Plasma or tissue homogenates 0.2-1.0 ml were placed in glass extraction tubes and water was added to make 1.0 ml. Each sample was extracted 3 times with 4 ml mixed organic solvent (n-pentane: dichloromethane = 1:1, vol:vol). The organic layers of 3 extractions were combined and evaporated. The residue was dissolved in 100-300 microliters of ethylacetate and spotted on TLC plates.

Author(s): 
Jiang, J. R.
Zou, C. D.
Shu, H. L.
Zeng, Y. L.
Publication Title: 
Journal of Chromatographic Science

A thin-layer chromatograpy (TLC) method is developed to analyze artemisinin (AT) and its derivatives, artemether (AM) and arteether (AE), using a silica-gel plate with a mobile phase containing pure chloroform. After development, all products are visualized after dipping in a 4-methoxybenzaldehyde dipping reagent of 1% (v/v) in an acidic solution of sulphuric acid (98%, v/v) and acetic acid (96-98%, v/v) (respectively, 2% and 10%, v/v in alcohol-water, 60:30, v/v), presenting a purple color against a slightly colored background.

Author(s): 
Gabriëls, M.
Plaizier-Vercammen, J. A.
Publication Title: 
Journal of Chromatographic Science

In this study a clear separation between seven analogues of artemisinin on thin-layer chromatography (TLC) is presented. The developed TLC method is carried out on a RP-C18 thin-layer plate using acetonitrile-water (50:25 v/v) as the mobile phase. Spots are visualized by derivatization with an acidified 4-methoxybenzaldehyde reagent in methanol-water.

Author(s): 
Gabriëls, M.
Plaizier-Vercammen, J.
Publication Title: 
Biochemical Pharmacology

The antimalarial trioxanes, exemplified by the naturally occurring sesquiterpene lactone artemisinin and its semi-synthetic derivatives, contain an endoperoxide pharmacophore that lends tremendous potency against Plasmodium parasites. Despite decades of research, their mechanism of action remains unresolved. A leading model of anti-plasmodial activity hypothesizes that iron-mediated cleavage of the endoperoxide bridge generates cytotoxic drug metabolites capable of damaging cellular macromolecules.

Author(s): 
Hartwig, Carmony L.
Rosenthal, Andrew S.
D'Angelo, John
Griffin, Carol E.
Posner, Gary H.
Cooper, Roland A.

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