Chromatography, Thin Layer

Publication Title: 
Journal of AOAC International

Ocimum sanctum (family Lamiaceae) is a reputed drug of Ayurveda, commonly known as Tulasi. In the present work, we quantified 4 marker compounds, viz., eugenol, luteolin, ursolic acid, and oleanolic acid, from the leaf of green and black varieties of O. sanctum using high-performance thin-layer chromatography (HPTLC) with densitometry.

Author(s): 
Anandjiwala, Sheetal
Kalola, Jyoti
Rajani, Mandapati
Publication Title: 
Journal of Biosciences

Myrrh (guggulu) oleoresin from the Commiphora mukul tree is an important component of antiarthritic drugs in Ayurvedic medicine. Clinical data suggest that elevated levels of hyaluronidase and collagenase type 2 enzymes contribute significantly to cartilage degradation. Triphala guggulu (TG) is a guggulu-based formulation used for the treatment of arthritis. We assessed the chondroprotective potential of TG by examining its effects on the activities of pure hyaluronidase and collagenase type 2 enzymes.

Author(s): 
Sumantran, Venil N.
Kulkarni, Asavari A.
Harsulkar, Abhay
Wele, Asmita
Koppikar, Soumya J.
Chandwaskar, Rucha
Gaire, Vishakha
Dalvi, Madhuri
Wagh, Ulhas V.
Publication Title: 
Chemical & Pharmaceutical Bulletin

Multiple shoot cultures of two experimental lines of Withania somnifera plants (RS-Selection-1 and RS-Selection-2) were established using nodal segments as explants. The hormonal combinations of benzyl adenine and kinetin not only influenced their morphogenetic response but also differentially modulated the level of biogeneration of withanolide A in the in vitro shoots of the two lines. Interestingly, withanolide-A, that was hardly detectable in the aerial parts of field-grown Withania somnifera (explant source), accumulated considerably in the in vitro shoot cultures of the plant.

Author(s): 
Sangwan, Rajender Singh
Chaurasiya, Narayan Das
Lal, Payare
Misra, Laxminarain
Uniyal, Girish Chandra
Tuli, Rakesh
Sangwan, Neelam Singh
Publication Title: 
Phytochemical analysis: PCA

A new, simple, sensitive, selective and precise HPTLC method has been developed for the determination of taraxerol in Clitoria ternatea L. Determination of taraxerol was performed on TLC aluminium plates. Linear ascending development was carried out in twin trough glass chamber saturated with hexane and ethyl acetate (80:20 v/v). The plate was then dried and sprayed with anisaldehyde reagent. A Camag TLC scanner III was used for spectrodensitometric scanning and analysis at 420 nm. The system was found to give compact spots for taraxerol (R(f) 0.53).

Author(s): 
Kumar, Venkatesan
Mukherjee, Kakali
Kumar, Satheesh
Mal, Mainak
Mukherjee, Pulok K.
Publication Title: 
Phytochemical analysis: PCA

Kutajarista is a well known polyherbal preparation of which the main ingredient is the stem bark of Holarrhena antidysenterica. This Ayurvedic medicine is prescribed to treat amoebic dysentery and other disorders such as fever, indigestion and malabsorption syndrome. Herbal medicines are very important since, in common with conventional medicines, they contain biologically active substances that may produce non-trivial side effects when taken in excessive amounts. Very low doses, on the other hand, may have no therapeutic value.

Author(s): 
Garg, Shivali
Bhutani, K. K.
Publication Title: 
Physiologia Plantarum

Ashwagandha (Withania somnifera Dunal., Solanaceae) is one of the most reputed medicinal plants of Ayurveda, the traditional medical system. Several of its traditionally proclaimed medicinal properties have been corroborated by recent molecular pharmacological investigations and have been shown to be associated with its specific secondary metabolites known as withanolides, the novel group of ergostane skeletal phytosteroids named after the plant.

Author(s): 
Sangwan, Rajender Singh
Das Chaurasiya, Narayan
Lal, Payare
Misra, Laxminarain
Tuli, Rakesh
Sangwan, Neelam Singh
Publication Title: 
Journal of Pharmaceutical and Biomedical Analysis

Sesamum (Sesamum indicum) seed and its oil have been in use in Indian traditional medicine, 'Ayurveda' since antiquity. However, there has been no attempt to standardize the polyherbal formulations containing sesamum oil as the main ingredient in terms of its active principle or marker compound. Biologically active lignans in sesamum oil are identified as the marker compound for the oil and its formulations.

Author(s): 
Sukumar, Divya
Arimboor, Ranjith
Arumughan, C.
Publication Title: 
Biomedical and environmental sciences: BES

OBJECTIVE: BacoMind (BM) is a standardized extract of Bacopa monnieri, which belongs to the family Scrophulariaceae and is a creeping annual plant found throughout the Indian subcontinent. It has been used by Ayurvedic medicinal practitioners in India for almost 3000 years and is classified as a medharasayana, a substance which improves memory and intellect. With the widespread traditional use as well as scientific validation of Bacopa monnieri for nootropic activity, a bioactive-rich unique phytochemical composition-BacoMind was developed from B.

Author(s): 
Deb, Dlpanwita Dutta
Kapoor, Preeti
Dighe, R. P.
Padmaja, R.
Anand, M. S.
D'Souza, P.
Deepak, M.
Murali, B.
Agarwal, Amit
Publication Title: 
Journal of AOAC International

A sensitive, simple, rapid, and efficient high-performance thin-layer chromatographic (HPTLC) method has been developed and validated for the analysis of berberine in marketed Ayurvedic formulations containing Berberis aristata DC for regulatory purposes. Chromatography of methanolic extracts of these formulations was performed on silica gel 60 F254 aluminum-backed TLC plates of 0.2 mm layer thickness. The plate was developed up to 66 mm with the ternary-mobile phase butanol-acetic acid-water (8 + 1 + 1, v/v/v) at 33 +/- 5 degrees C with 5 min of tank saturation.

Author(s): 
Rout, Kedar Kumar
Pradhan, Subhalaxmi
Mishra, Sagar Kumar
Publication Title: 
Journal of AOAC International

The present study aimed to standardize the Ayurvedic preparation Haridra Khanda containing Curcuma longa as a major ingredient. Various physicochemical parameters such as alcohol-soluble extractive, water-soluble extractive, total ash, and acid-insoluble ash were determined according to the Ayurvedic Pharmacopoeia of India. Microscopic evaluation of the formulation revealed the presence of various diagnostic cell structures of C. longa. Trace metal analysis indicated the absence of toxic metals such as As, Cd, Hg, and Pb.

Author(s): 
Rout, Kedar Kumar
Parida, Sagarika
Mishra, Sagar Kumar

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