Clone Cells

Publication Title: 
Biological & Pharmaceutical Bulletin

Gallic acid (GA) and chebulagic acid (CA) were isolated from the extract of a herbal medicine, kashi (myrobalans: the fruit of Terminalia chebula) as active principles that blocked the cytotoxic T lymphocyte (CTL)-mediated cytotoxicity. GA and CA inhibited the killing activity of CD8+ CTL clone at IC50 values of 30 microM and 50 microM, respectively. Granule exocytosis in response to anti-CD3 stimulation was also blocked by GA and CA at the equivalent concentrations.

Author(s): 
Hamada, S.
Kataoka, T.
Woo, J. T.
Yamada, A.
Yoshida, T.
Nishimura, T.
Otake, N.
Nagai, K.
Publication Title: 
Mechanisms of Ageing and Development

Carnosine (beta-alanyl-L-histidine), an abundant naturally-occurring dipeptide has been shown to exhibit anti-ageing properties towards cultured cells, possibly due in part to its antioxidant/free radical scavenging abilities. In this paper the results of an investigation on the effects of carnosine, at the physiological concentration of 20 mM, on oxidative DNA damage levels and in vitro lifespan in peripheral blood derived human CD4+ T cell clones are reported.

Author(s): 
Hyland, P.
Duggan, O.
Hipkiss, A.
Barnett, C.
Barnett, Y.
Publication Title: 
Oncogene

Replicative senescence is thought to be a significant barrier to human tumorigenesis, which in human fibroblasts, and many other cell types, can be overcome experimentally by combined loss of function of p53 and Rb 'pathways'. To avoid the confounding pleiotropic effects of HPVE7 frequently used in such studies, here we have employed retroviral vectors over-expressing CDK4 or CDK6 as a more representative model of naturally-occurring mutations targeting the Rb pathway.

Author(s): 
Morris, Mark
Hepburn, Peter
Wynford-Thomas, David
Publication Title: 
Blood

Heterozygous mutations of the human telomerase RNA template gene (TERC) have been described in patients with acquired aplastic anemia and the autosomal dominant form of dyskeratosis congenita (DKC). Patients with mutations in both TERC alleles have not yet been reported. Here, we report a patient with DKC who inherited 2 distinct TERC sequence variants from her parents; a deletion (216_229del) in one and a point mutation (37A>G) in the other allele of the TERC gene. Her marrow was hypocellular and showed an abnormal clone [46, XX t(7;21)(q34;q22)].

Author(s): 
Ly, Hinh
Schertzer, Mike
Jastaniah, Wasil
Davis, Jeff
Yong, Siu Li
Ouyang, Qin
Blackburn, Elizabeth H.
Parslow, Tristram G.
Lansdorp, Peter M.
Publication Title: 
Schizophrenia Research

This work is dedicated to the exploration of the role of epigenetic (epiG) factors in major psychosis. One of the key functions of epigenetic modification of the genome of eukaryotic cells is to suppress transcriptional activity of the retroelements. Examples of retroelements are endogenous retroviral sequences (ERVs), Alu's, and LINEs, among others, which as a rule are hypermethylated. There is evidence from schizophrenia (SCH) and other human complex diseases that some of the genomic retroelements become transcribed in the affected tissues.

Author(s): 
Kan, Pei-Xiang
Popendikyte, Violeta
Kaminsky, Zachary A.
Yolken, Robert H.
Petronis, Arturas
Publication Title: 
Cellular Physiology and Biochemistry: International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology

Little is known about the relationship between CF transmembrane conductance regulator (CFTR) gene expression and the corresponding transport of Cl. The phenotypic characteristics of polarized DeltaF508 homozygote CF bronchial epithelial (CFBE41o-) cells were evaluated following transfection with episomal expression vector containing either full-length (6.2kb) wild type (wt) and (4.7kb) DeltaF508CFTR cDNA.

Author(s): 
Illek, Beate
Maurisse, Rosalie
Wahler, Logan
Kunzelmann, Karl
Fischer, Horst
Gruenert, Dieter C.
Publication Title: 
European Journal of Pharmacology

We recently found that genistein, a plant-derived natural compound, is a novel cAMP signaling agonist in pancreatic beta-cells. In the present study, we further show that exposure of clonal insulin secreting (INS-1E) cells to genistein for 48 h enhanced glucose-stimulated insulin secretion (GSIS), whereas insulin content was not altered, suggesting that genistein-enhanced GSIS is not due to a modulation of insulin synthesis. This genistein effect is protein tyrosine kinase- and K(ATP) channel-independent.

Author(s): 
Fu, Zhuo
Liu, Dongmin
Publication Title: 
Cancer

BACKGROUND: Sarcomatoid features in renal cell carcinoma may represent an aggressive subclone arising from the primary tumor. The patterns of metastases for these tumors were evaluated to determine if sarcomatoid features were retained at metastasis and whether the percentage of sarcomatoid features in the primary tumor influenced spread. METHODS: All patients with sarcomatoid features found at nephrectomy with synchronous or metachronous resection of metastases were evaluated. The histology, grade, and percentage of sarcomatoid features in the primary and metastatic site were recorded.

Author(s): 
Shuch, Brian
Said, Jonathan
LaRochelle, Jeffrey C.
Zhou, Ying
Li, Gang
Klatte, Tobias
Pouliot, Frederic
Kabbinavar, Fairooz F.
Belldegrun, Arie S.
Pantuck, Allan J.
Publication Title: 
Journal of Immunology (Baltimore, Md.: 1950)

Leukotrienes generated by 5-lipoxygenase (5-LOX)-catalyzed reaction are key regulators of inflammation. In ionophore-stimulated (A23187; 1-2.5 μM) human blood neutrophils or differentiated HL-60 cells, vitamin E forms differentially inhibited leukotriene B(4) (LTB(4)) with an IC(50) of 5-20 μM for γ-tocopherol, δ-tocopherol (δT), and γ-tocotrienol, but a much higher IC(50) for α-tocopherol.

Author(s): 
Jiang, Ziying
Yin, Xinmin
Jiang, Qing
Publication Title: 
Journal of Immunology (Baltimore, Md.: 1950)

Quantitative evaluation of T cell responses of patients receiving antitumoral vaccination with a protein is difficult because of the large number of possible HLA-peptide combinations that could be targeted by the response.

Author(s): 
Zhang, Yi
Sun, Zhaojun
Nicolay, Hugues
Meyer, Ralf G.
Renkvist, Nicolina
Stroobant, Vincent
Corthals, Jurgen
Carrasco, Javier
Eggermont, Alexander M. M.
Marchand, Marie
Thielemans, Kris
Wölfel, Thomas
Boon, Thierry
van der Bruggen, Pierre

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