Colony-Forming Units Assay

Publication Title: 
Bioscience, Biotechnology, and Biochemistry

Transfection of nearly senesced human fibroblasts with plasmids encoding HPV16 E6 protein or dominant-negative p53 mutants greatly increased their colony-forming ability. Isolated colonies with these plasmids showed extension of lifespan compared to those with a control plasmid. These data demonstrate that p53 plays a major role in senescence in normal human fibroblasts.

Author(s): 
Ide, A.
Fujii, M.
Nakababashi, K.
Ayusawa, D.
Publication Title: 
Experimental Dermatology

Dyskeratosis congenita (DC) is characterized by the triad of reticulate skin pigmentation, nail dystrophy and leukoplakia. Epidermal atrophy, hair growth defects, bone marrow failure and increased risk of cancer are also common in DC patients. DC is caused by mutations in genes encoding for telomerase complex factors. Although there is an association of epidermal abnormalities with DC, epidermal cells from DC donors have not been previously characterized.

Author(s): 
Gourronc, Francoise A.
Robertson, mckaylee M.
Herrig, Annie K.
Lansdorp, Peter M.
Goldman, Frederick D.
Klingelhutz, Aloysius J.
Publication Title: 
Cell Stem Cell

Calorie restriction (CR) extends life span and ameliorates age-related pathologies in most species studied, yet the mechanisms underlying these effects remain unclear. Using mouse skeletal muscle as a model, we show that CR acts in part by enhancing the function of tissue-specific stem cells. Even short-term CR significantly enhanced stem cell availability and activity in the muscle of young and old animals, in concert with an increase in mitochondrial abundance and induction of conserved metabolic and longevity regulators.

Author(s): 
Cerletti, Massimiliano
Jang, Young C.
Finley, Lydia W. S.
Haigis, Marcia C.
Wagers, Amy J.
Publication Title: 
Atherosclerosis

OBJECTIVE: Metabolic syndrome (MetS) is characterized by low-grade inflammation and confers an increased risk for cardiovascular disease. Endothelial progenitor cells (EPCs) are a measure of vascular health and are decreased in patients with various risk factors for cardiovascular disease (CVD). There is a paucity of data examining the EPC status especially in terms of their functionality in MetS subjects without diabetes or cardiovascular disease. We aimed to enumerate and functionally characterize EPCs in subjects with MetS in comparison to healthy controls.

Author(s): 
Jialal, I.
Devaraj, S.
Singh, U.
Huet, B. A.
Publication Title: 
Planta Medica

Echinacea species are used for beneficial effects on immune function, and various prevalent phytochemicals have immunomodulatory effects. Using a commercial E. purpurea (L.) Moench product, we have evaluated the myelopoietic effect on bone marrow of rats treated with various extracts and correlated this with their chemical class composition. Granulocyte/macrophage-colony forming cells (GM-CFCs) from femurs of female Sprague-Dawley rats were assessed at 24 h after 7 daily oral treatments.

Author(s): 
Ramasahayam, Sindhura
Baraka, Hany N.
Abdel Bar, Fatma M.
Abuasal, Bilal S.
Widrlechner, Mark P.
Sayed, Khalid A. El
Meyer, Sharon A.
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