Culture Media, Conditioned

Publication Title: 
Stem Cell Research & Therapy

Ageing is a biological certainty for all living organisms, and is due to the loss of tissue homeostasis and regenerative capacity (except for newts) in which somatic stem cells are thought to play an important role. Many ageing-associated dysfunctions in stem cells have been described, but it remains ambiguous whether these are merely an outcome of ageing or are causal. Parabiotic animal studies suggest there are factors in the systemic environment that can influence the regenerative capacity of tissues.

McCullagh, Karl J. A.
Publication Title: 
PloS One

Stem cells are increasingly the focus of translational research as well as having emerging roles in human cellular therapy. To support these uses there is a need for improved methods for in vivo cell localization and tracking. In this study, we examined the effects of cell labeling on the in vitro functionality of human adipose-derived mesenchymal stem cells. Our results provide a basis for future in vivo studies investigating implanted cell fate and longevity.

Blaber, Sinead P.
Hill, Cameron J.
Webster, Rebecca A.
Say, Jana M.
Brown, Louise J.
Wang, Shih-Chang
Vesey, Graham
Herbert, Benjamin Ross
Publication Title: 

The present study investigates the effect of in vivo administration of alcoholic extract of Tinospora cordifolia whole plant (ALTC) on the proliferation and myeloid differentiation of bone marrow hematopoietic precursor cells in mice bearing a transplantable T cell lymphoma of spontaneous origin designated as Dalton's lymphoma (DL). BMC obtained from ALTC administered DL-bearing mice showed an enhanced BMC proliferation and colony forming ability in vitro in response to L929 conditioned medium as a source of colony stimulating factor (CSF).

Singh, Sukh Mahendra
Singh, Nisha
Shrivastava, Pratima
Publication Title: 
The American Journal of Pathology

C-reactive protein (CRP) is a risk marker for cardiovascular events in apparently healthy persons. Cogent data show that, aside from the liver, CRP is produced in atherosclerotic lesions, kidney, neurons, and alveolar macrophages. Because several proatherogenic effects of CRP have been documented in endothelial cells, we examined human aortic endothelial cells (HAEC) for CRP production. We detected the presence of CRP mRNA by RT-PCR and in situ hybridization, intracellular protein by Western blot and secreted protein by ELISA.

Venugopal, Senthil Kumar
Devaraj, Sridevi
Jialal, Ishwarlal
Publication Title: 
The Journal of Biological Chemistry

Flavonoids have been suggested to exert human health benefits by anti-oxidant and anti-inflammatory mechanisms. In this study, we investigated whether and by what mechanisms dietary flavonoids inhibit expression of cellular adhesion molecules, which is relevant to inflammation and atherosclerosis. We found that the capacity of flavonoids to inhibit tumor necrosis factor alpha-induced adhesion molecule expression in human aortic endothelial cells was dependent on specific structural features of the flavonoids.

Lotito, Silvina B.
Frei, Balz
Publication Title: 
Journal of Leukocyte Biology

Inflammation is pivotal in atherosclerosis. M-CSF regulates macrophage growth and differentiation and plays a role in atherogenesis. C-reactive protein (CRP), a cardiovascular risk marker, may promote atherogenesis. However, the effects of CRP on M-CSF release and subsequent macrophage proliferation have not been examined previously. Human aortic endothelial cells (HAEC) were incubated with boiled CRP or native CRP 12.5, 25, and 50 microg/mL for 12-15 h, and M-CSF release was examined by flow cytometry and ELISA.

Devaraj, Sridevi
Yun, Jung-Mi
Duncan-Staley, Catherine
Jialal, Ishwarlal
Publication Title: 
Inflammatory Bowel Diseases

BACKGROUND: Bacteria play a role in inflammatory bowel disease and other forms of intestinal inflammation. Although much attention has focused on the search for a pathogen or inciting inflammatory bacteria, another possibility is a lack of beneficial bacteria that normally confer anti-inflammatory properties in the gut. The purpose of this study was to determine whether normal commensal bacteria could inhibit inflammatory pathways important in intestinal inflammation.

Petrof, Elaine O.
Claud, Erika C.
Sun, Jun
Abramova, Tatiana
Guo, Yuee
Waypa, Tonya S.
He, Shu-Mei
Nakagawa, Yasushi
Chang, Eugene B.
Publication Title: 
The Journal of Biological Chemistry

Mesenchymal stem cells (MSCs) are bone marrow stromal cells that can differentiate into multiple lineages. We previously demonstrated that BMP9 is one of the most potent BMPs to induce osteogenic differentiation of MSCs. BMP9 is one of the least studied BMPs. Whereas ALK1, ALK5, and/or endoglin have recently been reported as potential BMP9 type I receptors in endothelial cells, little is known about type I receptor involvement in BMP9-induced osteogenic differentiation in MSCs.

Luo, Jinyong
Tang, Min
Huang, Jiayi
He, Bai-Cheng
Gao, Jian-Li
Chen, Liang
Zuo, Guo-Wei
Zhang, Wenli
Luo, Qing
Shi, Qiong
Zhang, Bing-Qiang
Bi, Yang
Luo, Xiaoji
Jiang, Wei
Su, Yuxi
Shen, Jikun
Kim, Stephanie H.
Huang, Enyi
Gao, Yanhong
Zhou, Jian-Zhong
Yang, Ke
Luu, Hue H.
Pan, Xiaochuan
Haydon, Rex C.
Deng, Zhong-Liang
He, Tong-Chuan
Publication Title: 
The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences

There is increasing evidence that age-associated chronic low-grade inflammation promotes the development of both large-vessel disease (myocardial infarction, stroke, peripheral arterial disease) and small-vessel pathologies (including vascular cognitive impairment) in older persons. However, the source of age-related chronic vascular inflammation remains unclear.

Csiszar, Anna
Sosnowska, Danuta
Wang, Mingyi
Lakatta, Edward G.
Sonntag, William E.
Ungvari, Zoltan
Publication Title: 
Journal of Lipid Research

12/15 lipoxygenase (12/15LO) oxidizes polyunsaturated fatty acids (PUFAs) to form bioactive lipid mediators. The role of 12/15LO in atherosclerosis development remains controversial. We evaluated atherosclerosis development and lipid metabolism in 12/15LO-LDL receptor (LDLr) double knockout (DK) vs. LDLr knockout (SK) mice fed a PUFA-enriched diet to enhance production of 12/15LO products.

Rong, Shunxing
Cao, Qiang
Liu, Mingxia
Seo, Jeongmin
Jia, Lin
Boudyguina, Elena
Gebre, Abraham K.
Colvin, Perry L.
Smith, Thomas L.
Murphy, Robert C.
Mishra, Nilamadhab
Parks, John S.


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