Cyclin D1

Publication Title: 
The Journal of Biological Chemistry

The mechanisms by which amino acids regulate the cell cycle are not well characterized. In this study, we examined the control of hepatocyte proliferation by amino acids and protein intake. In short-term culture, hepatocytes demonstrated normal entry into S phase and cell cycle protein expression in the absence of essential amino acids. However, deprivation of a set of nonessential amino acids (NEAA) potently inhibited cell cycle progression and selectively down-regulated the expression of proliferation-control proteins.

Author(s): 
Nelsen, Christopher J.
Rickheim, David G.
Tucker, Melissa M.
McKenzie, Travis J.
Hansen, Linda K.
Pestell, Richard G.
Albrecht, Jeffrey H.
Publication Title: 
Cell Cycle (Georgetown, Tex.)

Apigenin, a dietary plant-flavonoid has shown anti-proliferative and anticancer properties, however the molecular basis of this effect remains to be elucidated. We studied the molecular events of apigenin action in human prostate cancer cells. Treatment of LNCaP and PC-3 cells with apigenin causes G0-G1 phase arrest, decrease in total Rb protein and its phosphorylation at Ser780 and Ser807/811 in dose- and time-dependent fashion.

Author(s): 
Shukla, Sanjeev
Gupta, Sanjay
Publication Title: 
The Journal of Investigative Dermatology

IL-12 deficiency has been shown to promote photocarcinogenesis in mice. As UVB-induced inflammation is an important tumor-promoting event in the development of skin tumors, we determined the effects of IL-12-deficiency on UVB-induced inflammatory responses in mice. For this purpose, IL-12-knockout (IL-12 KO) and their wild-type counterparts were subjected to a photocarcinogenesis protocol; skin and tumor samples were collected at the termination of the experiment, and analyzed for biomarkers of inflammation and their mediators.

Author(s): 
Meeran, Syed M.
Punathil, Thejass
Katiyar, Santosh K.
Publication Title: 
The Journal of Investigative Dermatology

Consumption of green tea polyphenols (GTPs) in drinking water prevents photocarcinogenesis in mice; however, the molecular mechanisms underlying this effect have not been fully elucidated. Using IL-12p40 knockout (KO) mice and their wild-type counterparts and an established photocarcinogenesis protocol, we found that although administration of GTPs (0.2%, w/v) in drinking water significantly reduced UVB-induced tumor development in wild-type mice, this treatment had a nonsignificant effect in IL-12-KO mice.

Author(s): 
Meeran, Syed M.
Akhtar, Suhail
Katiyar, Santosh K.
Publication Title: 
Neoplasia (New York, N.Y.)

Interleukin-12 (IL-12)-deficiency promotes photocarcinogenesis in mice; however, the molecular mechanisms underlying this effect have not been fully elucidated. Here, we report that long-term exposure to ultraviolet (UV) radiation resulted in enhancement of the levels of cell survival kinases, such as phosphatidylinositol 3-kinase (PI3K), Akt (Ser(473)), p-ERK1/2, and p-p38 in the skin of IL-12p40 knockout (IL-12 KO) mice compared with the skin of wild-type mice. UV-induced activation of nuclear factor-kappaB (NF-kappaB)/p65 in the skin of IL-12 KO mice was also more prominent.

Author(s): 
Meeran, Syed M.
Katiyar, Nandan
Singh, Tripti
Katiyar, Santosh K.
Publication Title: 
Journal of Experimental Therapeutics & Oncology

Resveratrol (trans-3,4', 5-trihydroxystilbene) is a naturally occurring polyphenolic compound that has antiinflammatory, antioxidant, neuroprotective properties and acts as a chemopreventive agent. Resveratrol causes cell cycle arrest and induces apoptotic cell death in various types of cancer cells. In the current studies, the effect of resveratrol on phosphoinositide kinase-3 (PI3K)/protein kinase B (Akt)/mammalian target of rapamycin (mTOR) signaling pathway was examined in human U251 glioma cells. Resveratrol decreased both the expression and phosphorylation of Akt.

Author(s): 
Jiang, Hao
Shang, Xia
Wu, Hongtao
Gautam, Subhash C.
Al-Holou, Shaza
Li, Christina
Kuo, Jarret
Zhang, Lijie
Chopp, Michael
Publication Title: 
Neoplasia (New York, N.Y.)

Chemopreventive effects and associated mechanisms of grape seed extract (GSE) against intestinal/colon cancer development are largely unknown. Herein, we investigated GSE efficacy against intestinal tumorigenesis in APC(min/+) mice. Female APC(min/+) mice were fed control or 0.5% GSE (wt/wt) mixed AIN-76A diet for 6 weeks. At the end of the experiment, GSE feeding decreased the total number of intestinal polyps by 40%. The decrease in polyp formation in the small intestine was 42%, which was mostly in its middle (51%) and distal (49%) portions compared with the proximal one.

Author(s): 
Velmurugan, Balaiya
Singh, Rana P.
Kaul, Nidhi
Agarwal, Rajesh
Agarwal, Chapla
Publication Title: 
The Journal of Biological Chemistry

Human methionine adenosyltransferase 2beta (MAT2beta) encodes for two major splicing variants, V1 and V2, which are differentially expressed in normal tissues. Both variants are induced in human liver cancer and positively regulate growth. The aim of this work was to identify interacting proteins of V1 and V2. His-tagged V1 and V2 were overexpressed in Rosetta pLysS cells, purified, and used in a pulldown assay to identify interacting proteins from human colon cancer cell line RKO cell lysates. The eluted lysates were subjected to Western blot and in solution proteomic analyses.

Author(s): 
Xia, Meng
Chen, Yongheng
Wang, Ling-Chi
Zandi, Ebrahim
Yang, Heping
Bemanian, Sean
Martínez-Chantar, M. Luz
Mato, José M.
Lu, Shelly C.
Publication Title: 
Journal of Immunology (Baltimore, Md.: 1950)

Signals generated by the dynamic mechanical strain critically regulate endothelial cell proliferation and angiogenesis; however, the molecular basis remains unclear. We investigated the mechanisms by which human dermal microvascular endothelial cells (HDMECs) perceive mechanical signals and relay them intracellularly to regulate gene expression and endothelial cell proliferation. HDMECs were exposed to low/physiologic levels of dynamic strain and probed for the differential activation/inhibition of kinases in the mechanosignaling cascade associated with endothelial cell gene activation.

Author(s): 
Liu, Jie
Agarwal, Sudha
Publication Title: 
Molecular Carcinogenesis

Chemoprevention by dietary agents/supplements has emerged as a novel approach to control various malignancies, including colorectal cancer (CRC). This study assessed dietary grape seed extract (GSE) effectiveness in preventing azoxymethane (AOM)-induced aberrant crypt foci (ACF) formation and associated mechanisms in Fischer 344 rats. Six-week-old rats were injected with AOM, and fed control diet or the one supplemented with 0.25% or 0.5% (w/w) GSE in pre- and post-AOM or only post-AOM experimental protocols.

Author(s): 
Velmurugan, Balaiya
Singh, Rana P.
Agarwal, Rajesh
Agarwal, Chapla

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