Deuterium Oxide

Publication Title: 
Molecular & cellular proteomics: MCP

Calorie restriction (CR) promotes longevity. A prevalent mechanistic hypothesis explaining this effect suggests that protein degradation, including mitochondrial autophagy, is increased with CR, removing damaged proteins and improving cellular fitness. At steady state, increased catabolism must be balanced by increasing mitochondrial biogenesis and protein synthesis, resulting in faster protein replacement rates.

Author(s): 
Price, John C.
Khambatta, Cyrus F.
Li, Kelvin W.
Bruss, Matthew D.
Shankaran, Mahalakshmi
Dalidd, Marcy
Floreani, Nicholas A.
Roberts, Lindsay S.
Turner, Scott M.
Holmes, William E.
Hellerstein, Marc K.
Publication Title: 
The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences

Chronic inhibition of the protein synthesis regulator mTORC1 through rapamycin extends life span in mice, with longer extension in females than in males. Whether rapamycin treatment inhibits protein synthesis or whether it does so differently between sexes has not been examined. UM-HET3 mice were fed a control or rapamycin-supplemented (Rap) diet for 12 weeks. Protein synthesis in mixed, cytosolic (cyto), and mitochondrial (mito) fractions and DNA synthesis and mTORC1 signaling were determined in skeletal muscle, heart, and liver.

Author(s): 
Drake, Joshua C.
Peelor, Frederick F.
Biela, Laurie M.
Watkins, Molly K.
Miller, Richard A.
Hamilton, Karyn L.
Miller, Benjamin F.
Publication Title: 
Journal of Applied Physiology (Bethesda, Md.: 1985)

This paper describes a method of determining protein synthesis and turnover using in vivo labeling of protein with deuterated water and analysis of matrix-assisted laser desorption time-of-flight mass spectrometer (MALDI-TOF) spectrum. Protein synthesis is calculated using mass isotopomer distribution analysis instead of precursor to product amino acid enrichment ratio.

Author(s): 
Xiao, Gary Guishan
Garg, Meena
Lim, Shu
Wong, Derek
Go, Vay Liang
Lee, Wai-Nang Paul
Publication Title: 
The American Journal of Clinical Nutrition

BACKGROUND: The WHO recommends exclusive breastfeeding (EBF) for 6 mo after birth. However, the time at which breast milk ceases to provide adequate energy and nutrition, requiring the introduction of complementary foods, remains unclear. Most studies that investigated this issue were observational and potentially confounded by variability in social circumstances or infant growth. OBJECTIVE: We hypothesized that EBF infants would consume more breast milk at age 6 mo than infants receiving breast milk and complementary foods.

Author(s): 
Wells, Jonathan C. K.
Jonsdottir, Olof H.
Hibberd, Patricia L.
Fewtrell, Mary S.
Thorsdottir, Inga
Eaton, Simon
Lucas, Alan
Gunnlaugsson, Geir
Kleinman, Ronald E.
Publication Title: 
Homeopathy: The Journal of the Faculty of Homeopathy

The key stumbling block to serious consideration of homeopathy is the presumed "implausibility" of biological activity for homeopathic medicines in which the source material is diluted past Avogadro's number of molecules. Such an argument relies heavily on the assumptions of elementary chemistry (and biochemistry), in which the material composition of a solution, (dilution factors and ligand-receptor interactions), is the essential consideration.

Author(s): 
Rao, Manju Lata
Roy, Rustum
Bell, Iris R.
Hoover, Richard
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