Diacylglycerol O-Acyltransferase

Publication Title: 
Aging

Calorie restriction results in leanness, which is linked to metabolic conditions that favor longevity. We show here that deficiency of the triglyceride synthesis enzyme acyl CoA:diacylglycerol acyltransferase 1 (DGAT1), which promotes leanness, also extends longevity without limiting food intake. Female DGAT1-deficient mice were protected from age-related increases in body fat, tissue triglycerides, and inflammation in white adipose tissue. This protection was accompanied by increased mean and maximal life spans of ~25% and ~10%, respectively.

Author(s): 
Streeper, Ryan S.
Grueter, Carrie A.
Salomonis, Nathan
Cases, Sylvaine
Levin, Malin C.
Koliwad, Suneil K.
Zhou, Ping
Hirschey, Mattew D.
Verdin, Eric
Farese, Robert V.
Publication Title: 
Diabetes, Obesity & Metabolism

AIM: To elucidate the mechanism by which rosiglitazone regulates adipose triglyceride lipase (ATGL). METHODS: Male C57Bl/6 mice were treated with rosiglitazone daily (10 mg/kg body weight), and adipose tissues were weighed and preserved for mRNA and protein analysis of ATGL. In parallel, preadipocyte (3T3-L1) cells were differentiated with insulin/dexamethasone/3-isobutyl-1-methlxanthine cocktail or rosiglitazone, and ATGL levels were measured with real-time PCR, western blotting and immunohistochemistry.

Author(s): 
Liu, L.-F.
Purushotham, A.
Wendel, A. A.
Koba, K.
DeIuliis, J.
Lee, K.
Belury, M. A.
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