DNA Nucleotidyltransferases

Publication Title: 
Cell

We have analyzed the de novo telomere synthesis catalyzed by the enzyme telomere terminal transferase (telomerase) from Tetrahymena. Oligonucleotides representing the G-rich strand of telomeric sequences from five different organisms specifically primed the addition of TTGGGG repeats in vitro, suggesting that primer recognition may involve a DNA structure unique to these oligonucleotides. The sequence at the 3' end of the oligonucleotide primer specified the first nucleotide added in the reaction.

Author(s): 
Greider, C. W.
Blackburn, E. H.
Publication Title: 
The Journal of Biological Chemistry
Author(s): 
Blackburn, E. H.
Publication Title: 
Journal of Molecular Biology
Author(s): 
Air, G. M.
Blackburn, E. H.
Sanger, F.
Coulson, A. R.
Publication Title: 
Nature
Author(s): 
Blackburn, E. H.
Publication Title: 
The Journal of Biological Chemistry
Author(s): 
Blackburn, E. H.
Publication Title: 
Cell

We have found a novel activity in Tetrahymena cell free extracts that adds tandem TTGGGG repeats onto synthetic telomere primers. The single-stranded DNA oligonucleotides (TTGGGG)4 and TGTGTGGGTGTGTGGGTGTGTGGG, consisting of the Tetrahymena and yeast telomeric sequences respectively, each functioned as primers for elongation, while (CCCCAA)4 and two nontelomeric sequence DNA oligomers did not. Efficient synthesis of the TTGGGG repeats depended only on addition of micromolar concentrations of oligomer primer, dGTP, and dTTP to the extract.

Author(s): 
Greider, C. W.
Blackburn, E. H.
Publication Title: 
Journal of Molecular Biology
Author(s): 
Air, G. M.
Blackburn, E. H.
Sanger, F.
Coulson, A. R.
Publication Title: 
Cell

We have found a novel activity in Tetrahymena cell free extracts that adds tandem TTGGGG repeats onto synthetic telomere primers. The single-stranded DNA oligonucleotides (TTGGGG)4 and TGTGTGGGTGTGTGGGTGTGTGGG, consisting of the Tetrahymena and yeast telomeric sequences respectively, each functioned as primers for elongation, while (CCCCAA)4 and two nontelomeric sequence DNA oligomers did not. Efficient synthesis of the TTGGGG repeats depended only on addition of micromolar concentrations of oligomer primer, dGTP, and dTTP to the extract.

Author(s): 
Greider, C. W.
Blackburn, E. H.
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