DNA, Plant

Publication Title: 
Molecular genetics and genomics: MGG

Self-incompatibility in the genus Prunus is controlled by two genes at the S-locus, S-RNase and SFB. Both genes exhibit the high polymorphism and high sequence diversity characteristic of plant self-incompatibility systems. Deduced polypeptide sequences of three myrobalan and three domestic plum S-RNases showed over 97% identity with S-RNases from other Prunus species, including almond, sweet cherry, Japanese apricot and Japanese plum. The second intron, which is generally highly polymorphic between alleles was also remarkably well conserved within these S-allele pairs.

Author(s): 
Sutherland, Bruce G.
Tobutt, Kenneth R.
Robbins, Timothy P.
Publication Title: 
Gene

Isolation of intact and pure genomic DNA (gDNA) is essential for many molecular biology applications. It is difficult to isolate pure DNA from mature trees of hot and dry desert regions because of the accumulation of high level of polysaccharides, phenolic compounds, tannins etc. We hereby report the standardized protocol for the isolation and purification of gDNA from seven ecologically and medically important tree species of Combretaceae viz. Anogeissus (Anogeissus sericea var.

Author(s): 
Gupta, Amit Kumar
Harish, null
Rai, Manoj Kumar
Phulwaria, Mahendra
Shekhawat, Narpat Singh
Publication Title: 
Biological & Pharmaceutical Bulletin

Terminalia, a large genus of Combretaceae, is distributed in Tropical Asia, Africa, and America. Some Terminalia plants are used in folk medicine because they possess powerful medicinal properties. Dried fruits of Terminalia bellirica and Terminalia chebula are used as the main ingredient in Triphala, a famous polyherbal formulation in Ayurvedic medicine and Thai folk medicine, because of their laxative, detoxifying, and rejuvenating effects. To clarify the phylogenetic relationships of medicinal Terminalia species (T. bellirica, T. chebula, and T.

Author(s): 
Intharuksa, Aekkhaluck
Ando, Hirokazu
Miyake, Katsunori
Sirisa-Ard, Panee
Mikage, Masayuki
Sasaki, Yohei
Publication Title: 
FEBS letters

Artemisinin, a sesquiterpene lactone endoperoxide derived from the plant Artemisia annua, forms the basis of the most important treatments of malaria in use today. In an effort to elucidate the biosynthesis of artemisinin, an expressed sequence tag approach to identifying the relevant biosynthetic genes was undertaken using isolated glandular trichomes as a source of mRNA.

Author(s): 
Teoh, Keat H.
Polichuk, Devin R.
Reed, Darwin W.
Nowak, Goska
Covello, Patrick S.
Publication Title: 
BMC genomics

BACKGROUND: Glandular trichomes produce a wide variety of commercially important secondary metabolites in many plant species. The most prominent anti-malarial drug artemisinin, a sesquiterpene lactone, is produced in glandular trichomes of Artemisia annua. However, only limited genomic information is currently available in this non-model plant species. RESULTS: We present a global characterization of A. annua glandular trichome transcriptome using 454 pyrosequencing.

Author(s): 
Wang, Wei
Wang, Yejun
Zhang, Qing
Qi, Yan
Guo, Dianjing
Publication Title: 
Biological & Pharmaceutical Bulletin

Correct genotype identification of medicinal plant material remains important for botanical drug industry. Limitations of chemical and morphological approaches for authentication have generated need for newer methods in quality control of botanicals. The present study was carried out to develop DNA based marker for identification of Phyllanthus emblica LINN. A putative marker (1.1 kb) specific for P. emblica was identified by Random Amplified Polymorphic DNA (RAPD) technique. Sequence Characterized Amplified Region (SCAR) marker was developed from the RAPD amplicon.

Author(s): 
Dnyaneshwar, Warude
Preeti, Chavan
Kalpana, Joshi
Bhushan, Patwardhan
Publication Title: 
Biological & Pharmaceutical Bulletin

Correct genotype identification of medicinal plant material remains important for botanical drug industry. Limitations of chemical and morphological approaches for authentication have generated need for newer methods in quality control of botanicals. The present study was carried out to develop DNA based marker for identification of Phyllanthus emblica LINN. A putative marker (1.1 kb) specific for P. emblica was identified by Random Amplified Polymorphic DNA (RAPD) technique. Sequence Characterized Amplified Region (SCAR) marker was developed from the RAPD amplicon.

Author(s): 
Dnyaneshwar, Warude
Preeti, Chavan
Kalpana, Joshi
Bhushan, Patwardhan
Publication Title: 
Biotechnology and Applied Biochemistry

Zingiber officinale Roscoe (common or culinary ginger) is an official drug in Ayurvedic, Indian herbal, Chinese, Japanese, African and British Pharmacopoeias. The objective of the present study was to develop DNA-based markers that can be applied for the identification and differentiation of the commercially important plant Z. officinale Roscoe from the closely related species Zingiber zerumbet (pinecone, bitter or 'shampoo' ginger) and Zingiber cassumunar [cassumunar or plai (Thai) ginger].

Author(s): 
Chavan, Preeti
Warude, Dnyaneshwar
Joshi, Kalpana
Patwardhan, Bhushan
Publication Title: 
Fitoterapia

Withania somnifera is one of the most important medicinal plants of Ayurveda and finds extensive uses in Indian traditional herbal preparations. In this investigation, selected accessions of the plant were examined for diversity through RAPDs, isoenzymes, polypeptide polymorphism and withanolide profiles. The accessions clustered together with respect to their characteristic profile of major withanolides and represented withaferin A, withanone, withanolide D or withanolide A rich groups.

Author(s): 
Chaurasiya, Narayan D.
Sangwan, Rajender S.
Misra, Laxmi N.
Tuli, Rakesh
Sangwan, Neelam S.
Publication Title: 
Fitoterapia

Tribulus terrestris L. (Zygophyllaceae) is one of the highly traded raw drugs and also used as a stimulative food additive in Europe and USA. While, Ayurvedic Pharmacopoeia of India recognizes T. terrestris as Goksura, Tribulus lanuginosus and T. subramanyamii are also traded by the same name raising issues of quality control. The nuclear ribosomal RNA genes and ITS (internal transcribed spacer) sequence were used to develop species-specific DNA markers. The species-specific markers efficiently amplified 295bp for T. terrestris (TT1F and TT1R), 300bp for T.

Author(s): 
Balasubramani, Subramani Paranthaman
Murugan, Ramar
Ravikumar, Kaliamoorthy
Venkatasubramanian, Padma

Pages

Subscribe to RSS - DNA, Plant