Down-Regulation

Publication Title: 
Molecular and Cellular Biochemistry

Long-term clinical observations and ongoing studies have shown antitumor effects of external Qi of Yan Xin Qigong (YXQG-EQ) that originated from traditional Chinese medicine (TCM). In order to understand the molecular mechanisms underlying the antitumor effects of YXQG-EQ, we investigate the effects of YXQG-EQ on growth and apoptosis in androgen-independent prostate cancer PC3 cells. We found that exposure to YXQG-EQ led to G2/M arrest associated with reduced cyclin B1 expression and apoptosis in PC3 cells.

Author(s): 
Yan, Xin
Shen, Hua
Jiang, Hongjian
Zhang, Chengsheng
Hu, Dan
Wang, Jun
Wu, Xinqi
Publication Title: 
Cellular Physiology and Biochemistry: International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology

BACKGROUND/AIMS: Colorectal cancer (CRC) is the second leading cause of cancer death in the Western countries. Novel approaches of treatment are needed for CRC. The purpose of the present study was to investigate cytotoxic effect of external Qi of Yan Xin Qigong (YXQ-EQ) on human colorectal cancer cells. METHODS: The effect of YXQ-EQ on viability, cell cycle progression and apoptosis in colorectal cancer HT-29 cells was investigated.

Author(s): 
Yan, Xin
Shen, Hua
Jiang, Hongjian
Hu, Dan
Wang, Jun
Wu, Xinqi
Publication Title: 
Nature Neuroscience

A major unresolved issue in treating pain is the paradoxical hyperalgesia produced by the gold-standard analgesic morphine and other opiates. We found that hyperalgesia-inducing treatment with morphine resulted in downregulation of the K(+)-Cl(-) co-transporter KCC2, impairing Cl(-) homeostasis in rat spinal lamina l neurons. Restoring the anion equilibrium potential reversed the morphine-induced hyperalgesia without affecting tolerance. The hyperalgesia was also reversed by ablating spinal microglia.

Author(s): 
Ferrini, Francesco
Trang, Tuan
Mattioli, Theresa-Alexandra M.
Laffray, Sophie
Del'Guidice, Thomas
Lorenzo, Louis-Etienne
Castonguay, Annie
Doyon, Nicolas
Zhang, Wenbo
Godin, Antoine G.
Mohr, Daniela
Beggs, Simon
Vandal, Karen
Beaulieu, Jean-Martin
Cahill, Catherine M.
Salter, Michael W.
De Koninck, Yves
Publication Title: 
Zhongguo Yao Li Xue Bao = Acta Pharmacologica Sinica

To induce arthritis, the adjuvant with heat-killed Mycobacterium tuberculosis was injected into the ankle joint in rats. Local redness, swelling, hotness, pain, and motor dysfunction of the inflamed joint (as well as mental dullness) were observed 48 h after inoculation. At this time, the maximal binding capacity (Bmax) of muscarinic receptors in limbic system was increased, while the dissociation constant (Kd) was unchanged.

Author(s): 
Ma, X. F.
Duan-Mu, Z. X.
Yin, Q. Z.
Publication Title: 
World journal of gastroenterology: WJG

AIM: To investigate the regulatory effect of electroacupuncture (EA) at Zusanli (ST36) on tumor necrosis factor-alpha (TNF-alpha) in rats with ulcerative colitis (UC), and further elucidate the therapeutic mechanism of EA on UC. METHODS: Thirty-two male Sprague-Dawley (SD) rats were randomly divided into four groups (n=8): normal control group; UC control group; UC+ST36 group and UC+non-acupoint group. A solution containing ethanol and 2,4,6-trinitrobenzenesulfonic acid (TNBS) was instilled into the distal colon in the rat (at a dose of 100 mg/kg) to set up UC rat model.

Author(s): 
Tian, Li
Huang, Yu-Xin
Tian, Min
Gao, Wei
Chang, Qing
Publication Title: 
Human Brain Mapping

The concept that specific acupuncture points have salubrious effects on distant target organ systems is a salient feature of Traditional Chinese Medicine (TCM). In this study, we used a multiple-session experiment to test whether electroacupuncture stimulation at two TCM vision-related acupoints, UB 60 and GB 37, located on the leg, could produce fMRI signal changes in the occipital regions of the brain, and the specificity of this effect when compared with stimulation at an adjacent non-acupoint (NAP). Six normal, acupuncture naive subjects completed the study.

Author(s): 
Kong, Jian
Kaptchuk, Ted J.
Webb, Julia Megan
Kong, Jiang-Ti
Sasaki, Yuka
Polich, Ginger R.
Vangel, Mark G.
Kwong, Kenneth
Rosen, Bruce
Gollub, Randy L.
Publication Title: 
European Journal of Pain (London, England)

Cancer pain impairs the quality of life of cancer patients, but opioid intervention can cause significant side effects that further decrease quality of life. Although electroacupuncture (EA) has been used to treat cancer pain, its mechanisms are largely unknown. To examine its effects and underlying mechanisms on cancer pain, we injected AT-3.1 prostate cancer cells into the tibia to induce bone cancer in the male Copenhagen rat.

Author(s): 
Zhang, Rui-Xin
Li, Aihui
Liu, Bing
Wang, Linbo
Xin, Jiajia
Ren, Ke
Qiao, Jian-Tian
Berman, Brian M.
Lao, Lixing
Publication Title: 
World journal of gastroenterology: WJG

AIM: To investigate the effects of moxibustion on down-regulation of the colonic epithelial cell apoptosis and repair of the tight junctions in rats with Crohn's disease (CD). METHODS: Sixty male Sprague-Dawley rats were randomly divided into a normal control (NC) group, a model control (MC) group, an herbs-partitioned moxibustion (HPM) group, a mild-warm moxibustion (MWM) group and a salicylazosulphapyridine (SASP) group, with 12 rats in each group. The CD model rats were treated with trinitrobenzene sulphonic acid to induce intestinal inflammation.

Author(s): 
Bao, Chun-Hui
Wu, Lu-Yi
Shi, Yin
Wu, Huan-Gan
Liu, Hui-Rong
Zhang, Rong
Yu, Li-Qing
Wang, Jin-Hai
Publication Title: 
The Journal of Endocrinology

Electroacupuncture (EA) is used to treat chronic stress; however, its mechanism(s) of action in allaying stress remains unclear. The interplay of stress hormones of the hypothalamus-pituitary-adrenal axis (HPA) and the sympathetic nervous system (SNS) is critical in the stress response. Our objective was to determine whether EA at acupoint, stomach 36 (EA St₃₆) is effective in preventing chronic cold stress-induced increased hormone levels in the rat by examining four groups of animals, three of which were exposed to cold and one of which was a non-treatment control group.

Author(s): 
Eshkevari, Ladan
Permaul, Eva
Mulroney, Susan E.

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