Electron Spin Resonance Spectroscopy

Publication Title: 
Biological & Pharmaceutical Bulletin

Free radicals react with biological molecules and destroy the structure of cells, which eventually causes free-radical induced disease such as cancer, renal failure, aging, etc. In this study, 6 extracts and 4 pure compounds of Terminalia chebula RETZ. were investigated for anti-lipid peroxidation, anti-superoxide radical formation and free radical scavenging activities. The superoxide radical scavenging of the 4 pure compounds was further evaluated using electron spin resonance (ESR) spectrometry. The results showed that all tested extracts and pure compounds of T.

Author(s): 
Cheng, Hua-Yew
Lin, Ta-Chen
Yu, Kuo-Hua
Yang, Chien-Min
Lin, Chun-Ching
Publication Title: 
Archives of Toxicology

A hepatoprotective compound was isolated from the ethanolic extract of the fruits of Terminalia chebula Retz. by consecutive solvent partitioning, followed by silica gel and Sephadex LH-20 column chromatographies. The purified compound was identified as a mixture of chebulic acid and its minor isomer, neochebulic acid, with a ratio of 2:1 by spectroscopic analysis including 1D and 2D NMR and MS spectroscopy. To our knowledge, this is the first report on the protection of rat hepatocytes against oxidative toxicity by chebulic acid obtained from T. chebula Retz.

Author(s): 
Lee, Hyun-Sun
Jung, Sung-Hoon
Yun, Bong-Sik
Lee, Kwang-Won
Publication Title: 
Aging

Excessive production of reactive oxygen species (ROS) contributes to progression of atherosclerosis, at least in part by causing endothelial dysfunction and inflammatory activation. The class III histone deacetylase SIRT1 has been implicated in extension of lifespan. In the vasculature,SIRT1 gain-of-function using SIRT1 overexpression or activation has been shown to improve endothelial function in mice and rats via stimulation of endothelial nitric oxide (NO) synthase (eNOS). However, the effects of SIRT1 loss-of-function on the endothelium in atherosclerosis remain to be characterized.

Author(s): 
Stein, Sokrates
Sch‰fer, Nicola
Breitenstein, Alexander
Besler, Christian
Winnik, Stephan
Lohmann, Christine
Heinrich, Kathrin
Brokopp, Chad E.
Handschin, Christoph
Landmesser, Ulf
Tanner, Felix C.
L¸scher, Thomas F.
Matter, Christian M.
Publication Title: 
PloS One

BACKGROUND AND AIMS: Free radicals are implicated in the aetiology of some gastrointestinal disorders such as gastric ulcer, colorectal cancer and inflammatory bowel disease. In the present study we investigated the antioxidant and genoprotective activity of some rotenoids (i.e. boeravinones) isolated from the roots of Boerhaavia diffusa, a plant used in the Ayurvedic medicine for the treatment of diseases affecting the gastrointestinal tract.

Author(s): 
Aviello, Gabriella
Canadanovic-Brunet, Jasna M.
Milic, Natasa
Capasso, Raffaele
Fattorusso, Ernesto
Taglialatela-Scafati, Orazio
Fasolino, Ines
Izzo, Angelo A.
Borrelli, Francesca
Publication Title: 
PloS One

BACKGROUND AND AIMS: Free radicals are implicated in the aetiology of some gastrointestinal disorders such as gastric ulcer, colorectal cancer and inflammatory bowel disease. In the present study we investigated the antioxidant and genoprotective activity of some rotenoids (i.e. boeravinones) isolated from the roots of Boerhaavia diffusa, a plant used in the Ayurvedic medicine for the treatment of diseases affecting the gastrointestinal tract.

Author(s): 
Aviello, Gabriella
Canadanovic-Brunet, Jasna M.
Milic, Natasa
Capasso, Raffaele
Fattorusso, Ernesto
Taglialatela-Scafati, Orazio
Fasolino, Ines
Izzo, Angelo A.
Borrelli, Francesca
Publication Title: 
Proceedings of the National Academy of Sciences of the United States of America

The metal-independent decomposition of organic hydroperoxides and the formation of organic alkoxyl radicals in the absence or presence of halogenated quinones were studied with electron spin resonance (ESR) and the spin-trapping agent 5,5-dimethyl-1-pyrroline N-oxide (DMPO). We found that 2,5-dichloro-1,4-benzoquinone (DCBQ) markedly enhanced the decomposition of tert-butylhydroperoxide (t-BuOOH), leading to the formation of the DMPO adducts with t-butoxyl radicals (t-BuO* and methyl radicals *CH(3)).

Author(s): 
Zhu, Ben-Zhan
Zhao, Hong-Tao
Kalyanaraman, Balaraman
Liu, Jun
Shan, Guo-Qiang
Du, Yu-Guo
Frei, Balz
Publication Title: 
European Journal of Pharmacology

Since flavonoids scavenge reactive oxygen species, they may potentially protect against ischemia/reperfusion injury. This study compared the scavenging capacity of specific flavonoids towards different reactive oxygen species. Whether the differential oxidant scavenging capacity correlated with their protective efficacy in ischemia/reperfusion injury of cardiomyocytes was determined.

Author(s): 
Chang, Wei-Tien
Shao, Zuo-Hui
Yin, Jun-Jie
Mehendale, Sangeeta
Wang, Chong-Zhi
Qin, Yimin
Li, Juan
Chen, Wen-Jone
Chien, Chiang-Ting
Becker, Lance B.
Vanden Hoek, Terry L.
Yuan, Chun-Su
Publication Title: 
Archives of Pharmacal Research

Reactive oxygen species (ROS) can induce oxidative injury via iron interactions (i.e. Fenton chemistry and hydroxyl radical formation). Our prior work suggested that American ginseng berry extract and ginsenoside Re were highly cardioprotective against oxidant stress. To extend this study, we evaluated the protective effect of protopanaxadiol-type ginsenoside Rb1 (gRb1) on H(2)O(2)-induced oxidative injury in cardiomyocytes and explored the ROS-mediated intracellular signaling mechanism. Cultured embryonic chick cardiomyocytes (4-5 day) were used.

Author(s): 
Li, Jing
Shao, Zuo-Hui
Xie, Jing-Tian
Wang, Chong-Zhi
Ramachandran, Srinivasan
Yin, Jun-Jie
Aung, Han
Li, Chang-Qing
Qin, Gina
Vanden Hoek, Terry
Yuan, Chun-Su
Publication Title: 
Chemical & Pharmaceutical Bulletin

Inonotus obliquus (persoon) Pilat (Chaga, in Russia, kabanoanatake in Japan) is a fungus having been used as a folk medicine in Russia and said to have many health beneficial functions such as immune modulating and anti-cancer activities.

Author(s): 
Nakajima, Yuki
Sato, Yuzo
Konishi, Tetsuya
Publication Title: 
Nutrients

Abelmoschus esculentus (Malvaceae), Hibiscus acetosella (Malvaceae), Manihot esculenta Crantz (Euphorbiaceae) and Pteridium aquilinum (Dennstaedtiaceae) leaves are currently consumed as vegetables by migrants from sub-Saharan Africa living in Western Europe and by the people in the origin countries, where these plants are also used in the folk medicine. Manihot leaves are also eaten in Latin America and some Asian countries. This work investigated the capacity of aqueous extracts prepared from those vegetables to inhibit the peroxidation of a linoleic acid emulsion.

Author(s): 
Tsumbu, Cesar N.
Deby-Dupont, Ginette
Tits, Monique
Angenot, Luc
Franck, Thierry
Serteyn, Didier
Mouithys-Mickalad, Ange

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