Electrophoresis, Gel, Two-Dimensional

Publication Title: 
Malaria Journal

BACKGROUND: The emergence of Plasmodium falciparum resistance to most anti-malarial compounds has highlighted the urgency to develop new drugs and to clarify the mechanisms of anti-malarial drugs currently used. Among them, doxycycline is used alone for malaria chemoprophylaxis or in combination with quinine or artemisinin derivatives for malaria treatment. The molecular mechanisms of doxycycline action in P. falciparum have not yet been clearly defined, particularly at the protein level.

Author(s): 
Briolant, Sébastien
Almeras, Lionel
Belghazi, Maya
Boucomont-Chapeaublanc, Elodie
Wurtz, Nathalie
Fontaine, Albin
Granjeaud, Samuel
Fusaï, Thierry
Rogier, Christophe
Pradines, Bruno
Publication Title: 
PloS One

Glandular secreting trichomes (GSTs) are called biofactories because they are active in synthesizing, storing and secreting various types of plant secondary metabolites. As the most effective drug against malaria, artemisinin, a sesquiterpene lactone is derived from GSTs of Artemisia annua. However, low artemisinin content (0.001%~1.54% of dry weight) has hindered its wide application. We investigate the GST-expressed proteins in Artemisia annua using a comparative proteomics approach, aiming for a better understanding of the trichome proteome and arteminisin metabolism.

Author(s): 
Wu, Ting
Wang, Yejun
Guo, Dianjing
Publication Title: 
Journal of the National Cancer Institute

BACKGROUND: Efficacy of withaferin A (WA), an Ayurvedic medicine constituent, for prevention of mammary cancer and its associated mechanisms were investigated using mouse mammary tumor virus-neu (MMTV-neu) transgenic model. METHODS: Incidence and burden of mammary cancer and pulmonary metastasis were scored in female MMTV-neu mice after 28 weeks of intraperitoneal administration with 100 µg WA (three times/week) (n = 32) or vehicle (n = 29).

Author(s): 
Hahm, Eun-Ryeong
Lee, Joomin
Kim, Su-Hyeong
Sehrawat, Anuradha
Arlotti, Julie A.
Shiva, Sruti S.
Bhargava, Rohit
Singh, Shivendra V.
Publication Title: 
Journal of the National Cancer Institute

BACKGROUND: Efficacy of withaferin A (WA), an Ayurvedic medicine constituent, for prevention of mammary cancer and its associated mechanisms were investigated using mouse mammary tumor virus-neu (MMTV-neu) transgenic model. METHODS: Incidence and burden of mammary cancer and pulmonary metastasis were scored in female MMTV-neu mice after 28 weeks of intraperitoneal administration with 100 µg WA (three times/week) (n = 32) or vehicle (n = 29).

Author(s): 
Hahm, Eun-Ryeong
Lee, Joomin
Kim, Su-Hyeong
Sehrawat, Anuradha
Arlotti, Julie A.
Shiva, Sruti S.
Bhargava, Rohit
Singh, Shivendra V.
Publication Title: 
Journal of Biochemistry and Molecular Biology

The study of whole patterns of changes in protein expression and their modifications, or proteomics, presents both technological advances as well as formidable challenges to biological researchers. Nutrition research and the food sciences in general will be strongly influenced by the new knowledge generated by the proteomics approach. This review examines the different aspects of proteomics technologies, while emphasizing the value of consideration of "traditional" aspects of protein separation.

Author(s): 
Barnes, Stephen
Kim, Helen
Publication Title: 
The American Journal of Clinical Nutrition

Many similarities exist between research on combinatorial chemistry and natural products and research on dietary supplements and botanicals at the National Institutes of Health (NIH) Botanical Research Centers. The technologies used at the centers are similar to those used by other NIH-sponsored investigators. All centers rigorously examine the authenticity of botanical dietary supplements and determine the composition and concentrations of the phytochemicals therein, most often by liquid chromatography-mass spectrometry.

Author(s): 
Barnes, Stephen
Birt, Diane F.
Cassileth, Barrie R.
Cefalu, William T.
Chilton, Floyd H.
Farnsworth, Norman R.
Raskin, Ilya
van Breemen, Richard B.
Weaver, Connie M.
Publication Title: 
Molecular Vision

PURPOSE: To determine comparative effects of ultraviolet (UV)-A irradiation on structural and functional properties of wild type (WT) alphaB-crystallin and its three deamidated mutant proteins (alphaB-Asn78Asp, alphaB-Asn146Asp, and alphaB-Asn78/146Asp). METHODS: Three deamidated mutants previously generated from recombinant WT alphaB-crystallin, using a site-specific mutagenesis procedure as previously described [32], were used. The WT alphaB-crystallin and its three deamidated species were exposed to UV-A light (320-400 nm) at intensities of 20 or 50 J/cm(2).

Author(s): 
Mafia, Kerri
Gupta, Ratna
Kirk, Marion
Wilson, L.
Srivastava, O. P.
Barnes, Stephen
Publication Title: 
Analytical Chemistry

Pancreatic cancer MIA PaCa cells were cultured in the presence and absence of (15)N amino acids mixture for 72 h. During protein synthesis, the incorporation of (15)N amino acids results in a new mass isotopomer distribution in protein, which is approximated by the concatenation of two binomial distributions of (13)C and (15)N. The fraction of protein synthesis (FSR) can thus be determined from the relative intensities of the "labeled" (new) and the "unlabeled" (old) spectra.

Author(s): 
Zhao, Yingchun
Lee, Wai-Nang Paul
Lim, Shu
Go, Vay Liang
Xiao, Jing
Cao, Rui
Zhang, Hengwei
Recker, Robert Roy
Xiao, Gary Guishan
Publication Title: 
Pancreas

OBJECTIVES: A new method of determining protein turnover by labeling protein with N amino acids was used in conjunction with serum-free cell culture to profile secreted proteins that are released by MIA PaCa-2 pancreatic cancer cells in culture. METHODS: MIA PaCa-2 cells were first cultured in Dulbecco modified Eagle medium (Gibco by Invitrogen, Carlsbad, Calif) with 10% fetal bovine serum, then in serum-free modified Eagle medium with or without 50% N algal amino acid mixture. The effect of oxythiamine chloride on secreteome was studied.

Author(s): 
Xiao, Jing
Lee, Wai-Nang Paul
Zhao, Yingchun
Cao, Rui
Go, Vay Liang W.
Recker, Robert R.
Wang, Qi
Xiao, Gary Guishan
Publication Title: 
Journal of Proteome Research

Oxythiamine (OT), a transketolase inhibitor, is known to inhibit pancreatic cancer cell proliferation. In this study, we investigated the effect of inhibition of the transketolase pathway on signaling pathways in MIA PaCa cancer cells using in-house proteomic techniques. We hypothesized that OT alter protein phosphorylation thus affecting cell cycle arrest and cell proliferation. MIA PaCa-2 cells were cultured in media containing an algal (15)N amino acid mixture at 50% enrichment, with and without OT, to determine protein expression and synthesis.

Author(s): 
Zhang, Hengwei
Cao, Rui
Lee, Wai-Nang Paul
Deng, Caishu
Zhao, Yingchun
Lappe, Joan
Recker, Robert
Yen, Yun
Wang, Qi
Tsai, Ming-Ying
Go, Vay Liang
Xiao, Gary Guishan

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