Electrophoretic Mobility Shift Assay

Publication Title: 
Toxicology and Applied Pharmacology

The ID (inhibitor of differentiation or DNA binding) helix-loop-helix proteins are important mediators of cellular differentiation and proliferation in a variety of cell types through regulation of gene expression. Overexpression of the ID proteins in normal human keratinocytes results in extension of culture lifespan, indicating that these proteins are important for epidermal differentiation. Our hypothesis is that the ID proteins are targets of the retinoic acid signaling pathway in keratinocytes.

Author(s): 
Villano, C. M.
White, L. A.
Publication Title: 
Nature

The lengths of human telomeres, which protect chromosome ends from degradation and end fusions, are crucial determinants of cell lifespan. During embryogenesis and in cancer, the telomerase enzyme counteracts telomeric DNA shortening. As shown in cancer cells, human telomerase binds the shelterin component TPP1 at telomeres during the S phase of the cell cycle, and adds ~60 nucleotides in a single round of extension, after which telomerase is turned off by unknown mechanisms.

Author(s): 
Chen, Liuh-Yow
Redon, Sophie
Lingner, Joachim
Publication Title: 
Nature

The lengths of human telomeres, which protect chromosome ends from degradation and end fusions, are crucial determinants of cell lifespan. During embryogenesis and in cancer, the telomerase enzyme counteracts telomeric DNA shortening. As shown in cancer cells, human telomerase binds the shelterin component TPP1 at telomeres during the S phase of the cell cycle, and adds ~60 nucleotides in a single round of extension, after which telomerase is turned off by unknown mechanisms.

Author(s): 
Chen, Liuh-Yow
Redon, Sophie
Lingner, Joachim
Publication Title: 
Biogerontology

Insulin-induced PI3K/Akt activation is known to inhibit a family of Forkhead transcription factors (FOXO), which can lead to increased oxidative stress in several model organisms. One of major transcription factors activated by oxidative stress and responsible for the production of many proinflammatory cytokines is NF-kappaB. In the present study, We were carried out to determine the relationship between FOXO1 and NF-kappaB activation using HEK293T cells and aged kidney isolated from ad libitum fed (AL) and 40% calorie restriction (CR) rats.

Author(s): 
Kim, Dae Hyun
Kim, Ji Young
Yu, Byung Pal
Chung, Hae Young
Publication Title: 
PloS One

Breast cancer is the most common cancer and the second leading cause of cancer death in industrialized countries. Systemic treatment of breast cancer is effective at the beginning of therapy. However, after a variable period of time, progression occurs due to therapy resistance. Artesunate, clinically used as anti-malarial agent, has recently revealed remarkable anti-tumor activity offering a role as novel candidate for cancer chemotherapy. We analyzed the anti-tumor effects of artesunate in metastasizing breast carcinoma in vitro and in vivo.

Author(s): 
Bachmeier, Beatrice
Fichtner, Iduna
Killian, Peter H.
Kronski, Emanuel
Pfeffer, Ulrich
Efferth, Thomas
Publication Title: 
Journal of Ethnopharmacology

Semecarpus anacardium (SA) Linn. (family Anacardiaceae), is a plant well-known for its medicinal value in Ayurveda. The nut extracts of this plant have been traditionally used as antihelminthic, anti-fungal, anti-carcinogenic and in the treatment of nervous debilities and arthritis. In this study we have evaluated crude ethanolic extract of SA nuts for its anti-inflammatory activities in vitro using peripheral blood and synovial fluid mononuclear cells of healthy individuals and rheumatoid arthritis (RA) patients.

Author(s): 
Singh, Divya
Aggarwal, Amita
Mathias, Amrita
Naik, Sita
Publication Title: 
International Journal of Oncology

We previously established a bioassay method to screen for compounds that activate the promoter activity of p21(WAF1/CIP1), a potent inhibitor of cyclin-dependent kinases, in a p53-independent manner. As an activator of p21(WAF1/CIP1) promoter activity, we isolated cryptolepine (CLP: 5-methyl indolo (2,3b)-quiniine), an indoloquinoline alkaloid, from the traditional Ayurvedic medicinal plant Sida cordifolia. We show here that CLP induces the expression of p21(WAF1/CIP1) with growth arrest in p53-mutated human osteosarcoma MG63 cells.

Author(s): 
Matsui, Taka-aki
Sowa, Yoshihiro
Murata, Hiroaki
Takagi, Koichi
Nakanishi, Ryoko
Aoki, Shunji
Yoshikawa, Masayuki
Kobayashi, Motomasa
Sakabe, Tomoya
Kubo, Toshikazu
Sakai, Toshiyuki
Publication Title: 
Gene

The inducible IkappaB kinase (IKKi/IKKepsilon) is a recently described serine-threonine kinase that activates the transcription factors NFkappaB, interferon regulatory factor-3 (IRF3) and CCAAA/enhancer-binding protein (C/EBPdelta). Several inflammatory agents have been shown to induce the expression of the IKKi gene in macrophages and other cell types but the mechanism is unknown.

Author(s): 
Wang, Naizhen
Ahmed, Salahuddin
Haqqi, Tariq M.
Publication Title: 
Journal of Immunology (Baltimore, Md.: 1950)

High-mobility-group box 1 (HMGB1), a nuclear protein, has recently been identified as an important mediator of local and systemic inflammatory diseases when released into the extracellular milieu. Anti-inflammatory regulation by the stress response is an effective autoprotective mechanism when the host encounters harmful stimuli, but the mechanism of action remains incompletely delineated.

Author(s): 
Tang, Daolin
Kang, Rui
Xiao, Weimin
Wang, Haichao
Calderwood, Stuart K.
Xiao, Xianzhong
Publication Title: 
Toxicology and Applied Pharmacology

CD40 is a costimulatory molecule linking innate and adaptive immune responses to bacterial stimuli, as well as a critical regulator of functions of other costimulatory molecules. The mechanisms regulating lipopolysaccharide (LPS)-induced CD40 expression have not been adequately characterized in human monocytic cells. In this study we used a human monocytic cell line, THP-1, to investigate the possible mechanisms of CD40 expression following LPS exposure. Exposure to LPS resulted in a dose- and time-dependent increase in CD40 expression.

Author(s): 
Wu, Weidong
Alexis, Neil E.
Chen, Xian
Bromberg, Philip A.
Peden, David B.

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