Enzyme-Linked Immunosorbent Assay

Publication Title: 
Acta Virologica

The relative concentration of plum pox virus (PPV) in leaves and flowers of plum, damson, myrobalan, blackthorn, apricot and peach trees was determined by enzyme-linked immunosorbent assay (ELISA) and expressed as the lowest dilution with positive reaction. Significant differences in relative PPV concentration were found in leaves among individual Prunus species naturally or artificially infected with the virus. The highest relative PPV concentration was found in blackthorns (7.81 x 10(-4)), common plum and apricot (1.56 x 10(-3) for the both latters).

Author(s): 
Polák, J.
Publication Title: 
Journal of Ethnopharmacology

ETHNOPHARMACOLOGICAL RELEVANCE: Helicobacter pylori infection is associated with gastritis, peptic ulcer, and gastric cancer. Due to its high global prevalence and uprising resistance to available antibiotics, efforts are now directed to identify alternative source to treat and prevent associated disorders.

Author(s): 
Zaidi, Syed Faisal
Muhammad, Jibran Sualeh
Shahryar, Saeeda
Usmanghani, Khan
Gilani, Anwarul-Hassan
Jafri, Wasim
Sugiyama, Toshiro
Publication Title: 
Biological & Pharmaceutical Bulletin

Advanced glycation end-products (AGEs) have been implicated in the development of diabetic complications. We report the antiglycating activity of chebulic acid (CA), isolated from Terminalia chebula on breaking the cross-links of proteins induced by AGEs and inhibiting the formation of AGEs. Aminoguanidine (AG) reduced 50% of glycated bovine serum albumin (BSA) with glycolaldehyde (glycol-BSA)-induced cross-links of collagen at a concentration of 67.8 ± 2.5 mM, the level of CA required for exerting a similar antiglycating activity was 38.8 ± 0.5 µM.

Author(s): 
Lee, Ji-Young
Oh, Jun-Gu
Kim, Jin Sook
Lee, Kwang-Won
Publication Title: 
International Journal of Oncology

Granulocyte macrophage colony-stimulating factor (GM-CSF) is a key cytokine for the generation and stimulation of dendritic cells (DCs), and it may also play a pivotal role in promoting the survival of DCs. In this study, the feasibility of creating a cancer vaccine using DCs adenovirally transduced with the carcinoembryonic antigen (CEA) gene and the GM-CSF gene was examined. In addition, the effect of the co-transduction of GM-CSF gene on the lifespan of these genetically modified DCs was determined.

Author(s): 
Ojima, Toshiyasu
Iwahashi, Makoto
Nakamura, Masaki
Matsuda, Kenji
Nakamori, Mikihito
Ueda, Kentaro
Naka, Teiji
Katsuda, Masahiro
Miyazawa, Motoki
Yamaue, Hiroki
Publication Title: 
Journal of the Neurological Sciences

Amyotrophic lateral sclerosis (ALS) is a progressive disease which is caused by degeneration of motor neurons in the central nervous system. The incidence of ALS is higher in men than women, but the female advantage disappears with increased age. Here, we report evidence that the female advantage is due to the protective role of estrogen. In an ALS mouse model carrying the human Cu/Zn superoxide dismutase (hSOD1) G93A transgene, ovariectomy did not alter the onset age of the disease while reducing the female lifespan by 7 days and making it comparable to that of the male transgenic mice.

Author(s): 
Choi, Chan-Il
Lee, Young-Don
Gwag, Byoung Joo
Cho, Sung Ig
Kim, Sung-Soo
Suh-Kim, Haeyoung
Publication Title: 
Age (Dordrecht, Netherlands)

Throughout life, there is an aging of the immune system that causes impairment of its defense capability. Prevention or delay of this deterioration is considered crucial to maintain general health and increase longevity. We evaluated whether dietary supplementation with Lactobacillus delbrueckii subsp. bulgaricus 8481 could enhance the immune response in the elderly. This multi-center, double-blind, and placebo controlled study enrolled 61 elderly volunteers who were randomly assigned to receive either placebo or probiotics. Each capsule of probiotics contained at least 3?◊?10(7)? L.

Author(s): 
Moro-GarcÌa, Marco Antonio
Alonso-Arias, Rebeca
Baltadjieva, Maria
Fern·ndez BenÌtez, Carlos
Fern·ndez Barrial, Manuel Amadeo
DÌaz Ruis·nchez, Enrique
Alonso Santos, Ricardo
Alvarez S·nchez, Magdalena
Saavedra Mij·n, Juan
LÛpez-Larrea, Carlos
Publication Title: 
Neuromolecular Medicine

A common cause of amyotrophic lateral sclerosis is mutations in superoxide dismutase-1, which provoke the disease by an unknown mechanism. We have previously found that soluble hydrophobic misfolded mutant human superoxide dismutase-1 species are enriched in the vulnerable spinal cords of transgenic model mice. The levels were broadly inversely correlated with life spans, suggesting involvement in the pathogenesis.

Author(s): 
Zetterstrˆm, Per
Graffmo, Karin S.
Andersen, Peter M.
Br‰nnstrˆm, Thomas
Marklund, Stefan L.
Publication Title: 
Indian Journal of Pathology & Microbiology

BACKGROUND: Patients with thalassemia major are largely transfusion dependent and are thus exposed to a variety of risks such as transmission of infectious diseases, iron overload and alloimmunization. This study was performed to determine the prevalence of human immune deficiency virus (HIV), hepatitis B virus (HBV), hepatitis C virus (HCV) and red cell antibodies among multiple-transfused thalassemic patients in and around the national capital region.

Author(s): 
Makroo, Raj Nath
Arora, Jatinder Singh
Chowdhry, Mohit
Bhatia, Aakanksha
Thakur, Uday Kumar
Minimol, Antony
Publication Title: 
Antioxidants & Redox Signaling

Several studies have shown that both oxidative stress and inflammation are linked to the process of hypertension and that the immune system is also involved in this age-related process. More specifically, the oxygen stress related to immune system dysfunction seems to have a key role in senescence, in agreement with the oxidation/ inflammation theory of aging. From a practical point of view, and according to our own research, the immune functions change in a similar fashion in hypertension and aging.

Author(s): 
De la Fuente, M.
Hernanz, A.
Vallejo, M. C.
Publication Title: 
Biogerontology

Insulin-induced PI3K/Akt activation is known to inhibit a family of Forkhead transcription factors (FOXO), which can lead to increased oxidative stress in several model organisms. One of major transcription factors activated by oxidative stress and responsible for the production of many proinflammatory cytokines is NF-kappaB. In the present study, We were carried out to determine the relationship between FOXO1 and NF-kappaB activation using HEK293T cells and aged kidney isolated from ad libitum fed (AL) and 40% calorie restriction (CR) rats.

Author(s): 
Kim, Dae Hyun
Kim, Ji Young
Yu, Byung Pal
Chung, Hae Young

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