False Positive Reactions

Publication Title: 
Lancet (London, England)

We tested, under independent conditions, the reproducibility of evidence from two previous trials that homoeopathy differs from placebo. The test model was again homoeopathic immunotherapy. 28 patients with allergic asthma, most of them sensitive to house-dust mite, were randomly allocated to receive either oral homoeopathic immunotherapy to their principal allergen or identical placebo. The test treatments were given as a complement to their unaltered conventional care. A daily visual analogue scale of overall symptom intensity was the outcome measure.

Author(s): 
Reilly, D.
Taylor, M. A.
Beattie, N. G.
Campbell, J. H.
McSharry, C.
Aitchison, T. C.
Carter, R.
Stevenson, R. D.
Publication Title: 
Journal of Postgraduate Medicine

In this article, we test the hypothesis that randomized clinical trials of acupuncture for pain with certain design features (A + B versus B) are likely to generate false positive results. Based on electronic searches in six databases, 13 studies were found that met our inclusion criteria. They all suggested that acupuncture is effected (one only showing a positive trend, all others had significant results). We conclude that the 'A + B versus B' design is prone to false positive results and discuss the design features that might prevent or exacerbate this problem.

Author(s): 
Ernst, E.
Lee, M. S.
Publication Title: 
Journal of Postgraduate Medicine

In this article, we test the hypothesis that randomized clinical trials of acupuncture for pain with certain design features (A + B versus B) are likely to generate false positive results. Based on electronic searches in six databases, 13 studies were found that met our inclusion criteria. They all suggested that acupuncture is effected (one only showing a positive trend, all others had significant results). We conclude that the 'A + B versus B' design is prone to false positive results and discuss the design features that might prevent or exacerbate this problem.

Author(s): 
Ernst, E.
Lee, M. S.
Publication Title: 
The American Journal of Tropical Medicine and Hygiene

We evaluated the ICT Malaria P.f./P.v. immunochromatographic test for the detection of the panmalarial antigen (PMA) using a rodent malaria model. Mice were infected with Plasmodium berghei by mosquito bite, and blood was examined by microscopy and the ICT test. Treatment with artemether was started when the parasite density exceeded 70,000/microL. The ICT PMA band appeared when the parasite density was more than 2,000/microL, but it continued to be positive after the parasitemia became negative in response to the drug treatment.

Author(s): 
Arai, Meiji
Ishii, Akira
Matsuoka, Hiroyuki
Publication Title: 
Malaria Journal

Conventional methods of assessing in-vitro antimalarial drug-concentration effect relationships in field testing of fresh isolates assess each parasite isolate individually. This leads to systematic overestimation of EC50 values for the most resistant isolates, and thus overestimation of the degree of resistance. In antimalarial drug-susceptibility studies conducted on the north-western border of Thailand the overestimation of EC50 for the most resistant isolate ranged from 15% for artesunate to 43% for mefloquine.

Author(s): 
Stepniewska, Kasia
Chotivanich, Kesinee
Brockman, Alan
Day, Nicholas P. J.
White, Nicholas J.
Publication Title: 
Malaria Journal

BACKGROUND: An assessment of the accuracy of Paracheck Pf, a malaria rapid diagnostic test (RDT) detecting histidine rich protein 2 was undertaken amongst children aged 6-59 months in eastern Democratic Republic of Congo. METHODS: This RDT assessment occurred in conjunction with an ACT efficacy trial. Febrile children were simultaneously screened with both RDT and high quality microscopy and those meeting inclusion criteria were followed for 35 days. RESULTS: 358 febrile children were screened with 180 children recruited for five weeks follow-up.

Author(s): 
Swarthout, Todd D.
Counihan, Helen
Senga, Raphael Kabangwa K.
van den Broek, Ingrid
Publication Title: 
Journal of Clinical Laboratory Analysis

Asian ginseng, Siberian ginseng, and Indian Ayurvedic medicine Ashwagandha demonstrated modest interference with serum digoxin measurements by the fluorescent polarization immunoassay (FPIA). Recently, Abbott Laboratories marketed a new digoxin immunoassay, Digoxin III for application on the AxSYM analyzer. We studied potential interference of these herbal supplements on serum digoxin measurement by Digoxin III assay in vitro and compared our results with the values obtained by Tina-quant assay.

Author(s): 
Dasgupta, Amitava
Tso, Gertie
Wells, Alice
Publication Title: 
Complementary Therapies in Nursing & Midwifery

The double-blind randomized clinical trial is the gold standard for trials of prayer and distant healing. Adequate blinding and randomization procedures should be followed and documented. The intervention must be well defined (include frequency, amount of time and training and/or experience level of healers). Subjects should have risks and benefits of study participation explained to them and sign informed consent before enrollment. Populations should be homogeneous. Consider stratification for smaller samples.

Author(s): 
Targ, Elisabeth
Publication Title: 
Complementary Therapies in Nursing & Midwifery

The double-blind randomized clinical trial is the gold standard for trials of prayer and distant healing. Adequate blinding and randomization procedures should be followed and documented. The intervention must be well defined (include frequency, amount of time and training and/or experience level of healers). Subjects should have risks and benefits of study participation explained to them and sign informed consent before enrollment. Populations should be homogeneous. Consider stratification for smaller samples.

Author(s): 
Targ, Elisabeth
Publication Title: 
Clinical Immunology (Orlando, Fla.)

Antinuclear antibodies (ANA) are a hallmark of systemic lupus erythematosus (SLE) and one of its key diagnostic criteria. Recently, new technologies based on antibody binding to fluorescence beads (FB) have been widely employed for ANA screening. We conducted a formal study in 385 consecutive patients who underwent both traditional immunofluorescence (IF) and FB testing and evaluated each patient for the diagnosis of SLE. The distribution of ANA test results was significantly different (chi(2)=73.12; p<0.0001) due to a marked discordance of double-negative and double-positive results.

Author(s): 
Bonilla, Eduardo
Francis, Lisa
Allam, Fatme
Ogrinc, Meribeth
Neupane, Hom
Phillips, Paul E.
Perl, Andras

Pages

Subscribe to RSS - False Positive Reactions