Glycosylation

Publication Title: 
Pharmaceutical Biology

CONTEXT: Protein glycation is the major contributing factor in the development of diabetic complications. The antiglycation potential of medicinal plants provides a promising opportunity as complementary interventions for complications. OBJECTIVE: To investigate the antiglycation potential of 19 medicinal plants extracts using albumin by estimating different indicators: (1) glycation (early and late), (2) albumin oxidation, and (3) amyloid aggregation.

Author(s): 
Tupe, Rashmi S.
Kemse, Nisha G.
Khaire, Amrita A.
Shaikh, Shamim A.
Publication Title: 
Medical Hypotheses

Life prolongation with dietary restriction can be found in much of the animal kingdom and thus is a phenomenon that is evolutionarily conserved. We propose that one benefit of this is protection of the genome at lower dietary intake, perhaps by reduction of glycosylation related alterations and retardation of age related impairment of DNA repair.

Author(s): 
Spencer, R. P.
Publication Title: 
American Journal of Physiology. Gastrointestinal and Liver Physiology

Aquaporin 11 (AQP11) is a protein channel expressed intracellularly in multiple organs, yet its physiological function is unclear. Aqp11 knockout (KO) mice die early due to malfunction of the kidney, a result of hydropic degeneration of proximal tubule cells. Here we report the generation of liver-specific Aqp11 KO mice, allowing us to study the role of AQP11 protein in liver of mice with normal kidney function. The unchallenged liver-specific Aqp11 KO mice have normal longevity, their livers appeared normal, and the plasma biochemistries revealed only a minor defect in lipid handling.

Author(s): 
Rojek, Aleksandra
F¸chtbauer, Ernst-Martin
F¸chtbauer, Annette
Jelen, Sabina
Malmendal, Anders
Fenton, Robert A.
Nielsen, S¯ren
Publication Title: 
Circulation

BACKGROUND: Although diabetes confers an increased propensity toward accelerated atherogenesis, data are lacking on monocyte activity in type 2 diabetic patients with (DM2-MV) and without (DM2) macrovascular disease compared with control subjects.

Author(s): 
Devaraj, S.
Jialal, I.
Publication Title: 
Journal of Immunology (Baltimore, Md.: 1950)

Glucose serves as the major energy substrate and the main precursor for the synthesis of glycosaminoglycans in chondrocytes. Facilitated glucose transport represents the first rate-limiting step in glucose metabolism. This study examines molecular regulation of facilitated glucose transport in normal human articular chondrocytes by proinflammatory cytokines. IL-1beta and TNF-alpha, and to a lesser degree IL-6, accelerate facilitated glucose transport as measured by [(3)H]2-deoxyglucose uptake.

Author(s): 
Shikhman, A. R.
Brinson, D. C.
Valbracht, J.
Lotz, M. K.
Publication Title: 
Genomics

In humans, uridine 5'-diphosphate glucuronosyltransferase (UGT) operates in opposition to glucuronidase (GUS) to control activity of diverse metabolites such as hormones by reversible conjugation with glucuronic acid. Previous data revealed that, as in mammals, these enzymes are required for plant life in that a UGT from Pisum sativum (PsUGT1) controls plant development by opposing endogenous GUS activity thereby modulating the duration of the cell cycle.

Author(s): 
Woo, Ho-Hyung
Jeong, Byeong Ryong
Hirsch, Ann M.
Hawes, Martha C.
Publication Title: 
Endocrinology

We recently found that plasma membrane phosphatidylinositol 4,5-bisphosphate (PIP(2))-regulated filamentous actin (F-actin) polymerization was diminished in hyperinsulinemic cell culture models of insulin resistance. Here we delineated whether increased glucose flux through the hexosamine biosynthesis pathway (HBP) causes the PIP(2)/F-actin dysregulation and insulin resistance induced by hyperinsulinemia.

Author(s): 
Bhonagiri, Padma
Pattar, Guruprasad R.
Horvath, Emily M.
Habegger, Kirk M.
McCarthy, Alicia M.
Elmendorf, Jeffrey S.
Publication Title: 
PloS One

BACKGROUND: Biofilms contribute to the pathogenesis of many forms of Staphylococcus aureus infection. Treatment of these infections is complicated by intrinsic resistance to conventional antibiotics, thus creating an urgent need for strategies that can be used for the prevention and treatment of biofilm-associated infections. METHODOLOGY/PRINCIPAL FINDINGS: This study demonstrates that a botanical natural product composition (220D-F2) rich in ellagic acid and its derivatives can limit S. aureus biofilm formation to a degree that can be correlated with increased antibiotic susceptibility.

Author(s): 
Quave, Cassandra L.
Estévez-Carmona, Miriam
Compadre, Cesar M.
Hobby, Gerren
Hendrickson, Howard
Beenken, Karen E.
Smeltzer, Mark S.
Publication Title: 
Molecular & cellular proteomics: MCP

Antibody effector functions have been shown to be influenced by the structure of the Fc N-glycans. Here we studied the changes in plasma or serum IgG Fc N-glycosylation upon vaccination of 10 Caucasian adults and 10 African children. Serum/plasma IgG was purified by affinity chromatography prior to and at two time points after vaccination. Fc N-glycosylation profiles of individual IgG subclasses were determined for both total IgG and affinity-purified anti-vaccine IgG using a recently developed fast nanoliquid chromatography-electrospray ionization MS (LC-ESI-MS) method.

Author(s): 
Selman, Maurice H. J.
de Jong, Sanne E.
Soonawala, Darius
Kroon, Frank P.
Adegnika, Ayola Akim
Deelder, André M.
Hokke, Cornelis H.
Yazdanbakhsh, Maria
Wuhrer, Manfred
Publication Title: 
Molecular & cellular proteomics: MCP

Antibody effector functions have been shown to be influenced by the structure of the Fc N-glycans. Here we studied the changes in plasma or serum IgG Fc N-glycosylation upon vaccination of 10 Caucasian adults and 10 African children. Serum/plasma IgG was purified by affinity chromatography prior to and at two time points after vaccination. Fc N-glycosylation profiles of individual IgG subclasses were determined for both total IgG and affinity-purified anti-vaccine IgG using a recently developed fast nanoliquid chromatography-electrospray ionization MS (LC-ESI-MS) method.

Author(s): 
Selman, Maurice H. J.
de Jong, Sanne E.
Soonawala, Darius
Kroon, Frank P.
Adegnika, Ayola Akim
Deelder, André M.
Hokke, Cornelis H.
Yazdanbakhsh, Maria
Wuhrer, Manfred

Pages

Subscribe to RSS - Glycosylation