Green Fluorescent Proteins

Publication Title: 
Plant Cell Reports

Resistant rootstocks offer an alternative to pesticides for the control of soil pests. In Prunus spp., resistance loci to root-knot nematodes (RKN) have been mapped and a transformation method is needed to validate candidate genes. Our efforts have focused on the generation of transformed hairy-roots and composite plants appropriate for nematode infection assays. An efficient and reliable method using the A4R strain of Agrobacterium rhizogenes for the transformation of Prunus roots with an Egfp reporter gene is given.

Author(s): 
Bosselut, Nathalie
Van Ghelder, Cyril
Claverie, Michel
Voisin, Roger
Onesto, Jean-Paul
Rosso, Marie-Noëlle
Esmenjaud, Daniel
Publication Title: 
Investigative Ophthalmology & Visual Science

PURPOSE: To investigate the migratory and contractile behavior of isolated human corneal fibroblasts in fibrillar collagen matrices. METHODS: A telomerase-infected, extended-lifespan human corneal fibroblast cell line (HTK) was transfected by using a vector for enhanced green fluorescent protein (GFP)-alpha-actinin. Cells were plated at low density on top of or within 100-microm-thick fibrillar collagen lattices. After 18 hours to 7 days, time-lapse imaging was performed.

Author(s): 
Vishwanath, Mridula
Ma, Lisha
Otey, Carol A.
Jester, James V.
Petroll, W. Matthew
Publication Title: 
PloS One

Hormesis occurs when a low level stress elicits adaptive beneficial responses that protect against subsequent exposure to severe stress. Recent findings suggest that mild oxidative and thermal stress can extend lifespan by hormetic mechanisms. Here we show that the botanical pesticide plumbagin, while toxic to C. elegans nematodes at high doses, extends lifespan at low doses. Because plumbagin is a naphthoquinone that can generate free radicals in vivo, we investigated whether it extends lifespan by activating an adaptive cellular stress response pathway. The C.

Author(s): 
Hunt, Piper R.
Son, Tae Gen
Wilson, Mark A.
Yu, Quian-Sheng
Wood, William H.
Zhang, Yongqing
Becker, Kevin G.
Greig, Nigel H.
Mattson, Mark P.
Camandola, Simonetta
Wolkow, Catherine A.
Publication Title: 
CNS & neurological disorders drug targets

The neural protein ?-synuclein aggregates both in vivo and in vitro to form insoluble fibrils that are involved in Parkinson's disease pathogenesis. We have generated ?-synuclein/fluorescent-protein fusion constructs overexpressed in muscle cells of the nematode, Caenorhabdtis elegans. Green Fluorescent Protein (GFP) variants, Cerulean (C) or Venus (V), were fused to the C-terminus of human ?-synuclein (S); the resultant fusion genes were designated SV and SC, plus a CV fusion as well as S, C and V singly. The aggregation behavior of the purified fusion proteins (expressed in E.

Author(s): 
Bodhicharla, Rakesh
Nagarajan, Archana
Winter, Jody
Adenle, Ademola
Nazir, Aamir
Brady, Declan
Vere, Kelly
Richens, Jo
O'Shea, Paul
Bell, David R.
de Pomerai, David
Publication Title: 
Nature Communications

It is hypothesized that a common underlying mechanism links multiple neurodegenerative disorders. Here we show that transitional endoplasmic reticulum ATPase (TERA)/valosin-containing protein (VCP)/p97 directly binds to multiple polyglutamine disease proteins (huntingtin, ataxin-1, ataxin-7 and androgen receptor) via polyglutamine sequence. Although normal and mutant polyglutamine proteins interact with TERA/VCP/p97, only mutant proteins affect dynamism of TERA/VCP/p97.

Author(s): 
Fujita, Kyota
Nakamura, Yoko
Oka, Tsutomu
Ito, Hikaru
Tamura, Takuya
Tagawa, Kazuhiko
Sasabe, Toshikazu
Katsuta, Asuka
Motoki, Kazumi
Shiwaku, Hiroki
Sone, Masaki
Yoshida, Chisato
Katsuno, Masahisa
Eishi, Yoshinobu
Murata, Miho
Taylor, J. Paul
Wanker, Erich E.
Kono, Kazuteru
Tashiro, Satoshi
Sobue, Gen
La Spada, Albert R.
Okazawa, Hitoshi
Publication Title: 
Experimental Neurology

Among several genetic mutations known to cause amyotrophic lateral sclerosis (ALS), a hexanucleotide repeat expansion in the C9orf72 gene is the most common. In approximately 30% of C9orf72-ALS cases, 5-methylcytosine (5mC) levels within the C9orf72 promoter are increased, resulting in a modestly attenuated phenotype. The developmental timing of C9orf72 promoter hypermethylation and the reason why it occurs in only a subset of patients remain unknown.

Author(s): 
Esanov, Rustam
Belle, Kinsley C.
van Blitterswijk, Marka
Belzil, Veronique V.
Rademakers, Rosa
Dickson, Dennis W.
Petrucelli, Leonard
Boylan, Kevin B.
Dykxhoorn, Derek M.
Wuu, Joanne
Benatar, Michael
Wahlestedt, Claes
Zeier, Zane
Publication Title: 
The Journal of Neuroscience: The Official Journal of the Society for Neuroscience

Ketamine has important anesthetic, analgesic, and psychotropic actions. It is widely believed that NMDA receptor inhibition accounts for ketamine actions, but there remains a dearth of behavioral evidence to support this hypothesis. Here, we present an alternative, behaviorally relevant molecular substrate for anesthetic effects of ketamine: the HCN1 pacemaker channels that underlie a neuronal hyperpolarization-activated cationic current (I(h)).

Author(s): 
Chen, Xiangdong
Shu, Shaofang
Bayliss, Douglas A.
Publication Title: 
Proceedings of the National Academy of Sciences of the United States of America

Clinical studies and mathematical models predict that, to achieve malaria elimination, combination therapies will need to incorporate drugs that block the transmission of Plasmodium falciparum sexual stage parasites to mosquito vectors. Efforts to measure the activity of existing antimalarials on intraerythrocytic sexual stage gametocytes and identify transmission-blocking agents have, until now, been hindered by a lack of quantitative assays. Here, we report an experimental system using P.

Author(s): 
Adjalley, Sophie H.
Johnston, Geoffrey L.
Li, Tao
Eastman, Richard T.
Ekland, Eric H.
Eappen, Abraham G.
Richman, Adam
Sim, B. Kim Lee
Lee, Marcus C. S.
Hoffman, Stephen L.
Fidock, David A.
Publication Title: 
PloS One

Endophytic actinobacteria colonize internal tissues of their host plants and are considered as a rich and reliable source of diverse species and functional microorganisms. In this study, endophytic actinobacterial strain YIM 63111 was isolated from surface-sterilized tissue of the medicinal plant Artemisia annua. We identified strain YIM 63111 as a member of the genus Pseudonocardia. A. annua seedlings grown under both sterile and greenhouse conditions were inoculated with strain YIM 63111. The growth of A.

Author(s): 
Li, Jie
Zhao, Guo-Zhen
Varma, Ajit
Qin, Sheng
Xiong, Zhi
Huang, Hai-Yu
Zhu, Wen-Yong
Zhao, Li-Xing
Xu, Li-Hua
Zhang, Si
Li, Wen-Jun
Publication Title: 
Molecular Biology of the Cell

Increased expression of vascular endothelial growth factor (VEGF) contributes to the growth of many tumors by increasing angiogenesis. Although hypoxia is a potent inducer of VEGF, we previously showed that epidermal growth factor receptor amplification and loss of PTEN, both of which can increase phosphatidylinositol-3-kinase (PI3K) activity, increase VEGF expression.

Author(s): 
Pore, Nabendu
Liu, Shuang
Shu, Hui-Kuo
Li, Bin
Haas-Kogan, Daphne
Stokoe, David
Milanini-Mongiat, Julie
Pages, Gilles
O'Rourke, Donald M.
Bernhard, Eric
Maity, Amit

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