Biochemical and Biophysical Research Communications
Clusterin is a disulfide-linked heterodimeric glycoprotein that has been implicated in a variety of biological processes. Its expression has been shown to be elevated during cellular senescence and normal aging, but it is uncertain whether clusterin protects against aging or whether its expression is a consequence of aging. To investigate the functions of clusterin during organismal aging, we established transgenic Drosophila alleles to induce the expression of the secretory form of human clusterin (hClu(S)) using the Gal4/UAS system.
Reduced diet or reduced reproduction each extends lifespan in many animals. It is often thought that reduced reproduction and reduced diet may act through the same mechanisms. In grasshoppers, ovariectomy extends lifespan and reduces feeding to a level similar to that used for life extension by dietary restriction, further suggesting mechanistic overlap. Here, we measure the feeding rate of ovariectomized grasshoppers and, by manipulating feeding levels, create a sham-operated & dietary restricted group with matched daily feeding.
The objective of this research was to evaluate artificial diets that can be used to successfully culture the atlas silk moth, Attacus atlas L. (Lepidoptera: Saturniidae) indoors. Four plant species were evaluated as the basic component of each diet, barringtonia (Barringtonia asiatica), cheesewood (Nauclea orientalis), soursop (Annona muricata), and mahogany (Swietenia mahagoni). Evaluation of the nutritional value of each diet was determined by an analysis of the hemolymph proteins of sixth instars using the Folin-Ciocalteu assay.