Hep G2 Cells

Publication Title: 
Journal of Ethnopharmacology

ETHNOPHARMACOLOGICAL RELEVANCE: The fruits of Terminalia bellerica Roxb. (Combretaceae) and T. chebula Retz. (Combretaceae) are important components of triphala, a popular Ayurvedic formulation, for treating diabetes in Indian traditional medicine. AIM OF THE STUDY: The aim of this study was to evaluate the effects of the constituents of T. bellerica and T. chebula fruit extracts on PPAR? and PPAR? signaling/expression, cellular glucose uptake and adipogenesis. MATERIALS AND METHODS: PPAR? and PPAR?

Author(s): 
Yang, Min Hye
Vasquez, Yelkaira
Ali, Zulfiqar
Khan, Ikhlas A.
Khan, Shabana I.
Publication Title: 
Planta Medica

Medicinal plants are a rich source of ligands for nuclear receptors. The present study was aimed to screen a collection of plant extracts for PPAR?/?-activating properties and identify the active extract that can stimulate cellular glucose uptake without enhancing the adipogenesis. A reporter gene assay was performed to screen ethanolic extracts of 263 plant species, belonging to 94 families, for activation of PPAR? and PPAR?. Eight extracts showed activation of PPAR?, while 22 extracts showed activation of PPAR?.

Author(s): 
Yang, Min Hye
Avula, Bharathi
Smillie, Troy
Khan, Ikhlas A.
Khan, Shabana I.
Publication Title: 
PloS One

Hormesis occurs when a low level stress elicits adaptive beneficial responses that protect against subsequent exposure to severe stress. Recent findings suggest that mild oxidative and thermal stress can extend lifespan by hormetic mechanisms. Here we show that the botanical pesticide plumbagin, while toxic to C. elegans nematodes at high doses, extends lifespan at low doses. Because plumbagin is a naphthoquinone that can generate free radicals in vivo, we investigated whether it extends lifespan by activating an adaptive cellular stress response pathway. The C.

Author(s): 
Hunt, Piper R.
Son, Tae Gen
Wilson, Mark A.
Yu, Quian-Sheng
Wood, William H.
Zhang, Yongqing
Becker, Kevin G.
Greig, Nigel H.
Mattson, Mark P.
Camandola, Simonetta
Wolkow, Catherine A.
Publication Title: 
Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et MÈtabolisme

Compounds that delay aging in model organisms may be of significant interest to antiaging medicine, since these substances potentially provide pharmaceutical approaches to promote healthy lifespan in humans. The aim of the study was to test whether pharmaceutical concentrations of the glycolytic inhibitor lonidamine are capable of extending lifespan in a nematodal model organism for aging processes, the roundworm Caenorhabditis elegans. Several hundreds of adult C. elegans roundworms were maintained on agar plates and fed E. coli strain OP50 bacteria.

Author(s): 
Schmeisser, S.
Zarse, K.
Ristow, M.
Publication Title: 
Aging Cell

Arsenite is one of the most toxic chemical substances known and is assumed to exert detrimental effects on viability even at lowest concentrations. By contrast and unlike higher concentrations, we here find that exposure to low-dose arsenite promotes growth of cultured mammalian cells. In the nematode C. elegans, low-dose arsenite promotes resistance against thermal and chemical stressors and extends lifespan of this metazoan, whereas higher concentrations reduce longevity. While arsenite causes a transient increase in reactive oxygen species (ROS) levels in C.

Author(s): 
Schmeisser, Sebastian
Schmeisser, Kathrin
Weimer, Sandra
Groth, Marco
Priebe, Steffen
Fazius, Eugen
Kuhlow, Doreen
Pick, Denis
Einax, J¸rgen W.
Guthke, Reinhard
Platzer, Matthias
Zarse, Kim
Ristow, Michael
Publication Title: 
Oncology Reports

SIRT1 is the human orthologue of SIR2, a conserved NAD-dependent protein deacetylase that regulates longevity in yeast and in Caenorhabditis elegans. Overexpression of SIRT1 in cancer tissue, compared with normal tissue, has been demonstrated, suggesting that SIRT1 may act as a tumor promoter. The function of SIRT1 in liver cancer has not been elucidated. In the present study, SIRT1 re-expression or knockdown was induced in hepatoma cell lines and liver normal cell lines.

Author(s): 
Wang, Hanning
Liu, Hao
Chen, Kaiyun
Xiao, Jinfeng
He, Ke
Zhang, Jinqian
Xiang, Guoan
Publication Title: 
Cell Death and Differentiation

Terminally differentiated neutrophils are short-lived but the key effector cells of the innate immune response, and have a prominent role in the pathogenesis and propagation of many inflammatory diseases. Delayed apoptosis, which is responsible for their extended longevity, is critically dependent on a balance of intracellular survival versus pro-apoptotic proteins. Here, we elucidate the mechanism by which the cyclin-dependent kinase (CDK) inhibitor drugs such as R-roscovitine and DRB (5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole) mediate neutrophil apoptosis.

Author(s): 
Leitch, A. E.
Lucas, C. D.
Marwick, J. A.
Duffin, R.
Haslett, C.
Rossi, A. G.
Publication Title: 
Aging Cell

Arsenite is one of the most toxic chemical substances known and is assumed to exert detrimental effects on viability even at lowest concentrations. By contrast and unlike higher concentrations, we here find that exposure to low-dose arsenite promotes growth of cultured mammalian cells. In the nematode C. elegans, low-dose arsenite promotes resistance against thermal and chemical stressors and extends lifespan of this metazoan, whereas higher concentrations reduce longevity. While arsenite causes a transient increase in reactive oxygen species (ROS) levels in C.

Author(s): 
Schmeisser, Sebastian
Schmeisser, Kathrin
Weimer, Sandra
Groth, Marco
Priebe, Steffen
Fazius, Eugen
Kuhlow, Doreen
Pick, Denis
Einax, J¸rgen W.
Guthke, Reinhard
Platzer, Matthias
Zarse, Kim
Ristow, Michael
Publication Title: 
Methods in Molecular Biology (Clifton, N.J.)

Chronic hepatitis B remains a substantial public health burden affecting approximately 350 million people worldwide, causing cirrhosis and liver cancer, and about 1 million people die each year from hepatitis B and its complications. Hepatitis B is caused by hepatitis B virus (HBV) infection. As an essential component of the viral life cycle, HBV covalently closed circular DNA (cccDNA) is synthesized and maintained at low copy numbers in the nucleus of infected hepatocytes, and serves as the transcription template for all viral RNAs.

Author(s): 
Cai, Dawei
Nie, Hui
Yan, Ran
Guo, Ju-Tao
Block, Timothy M.
Guo, Haitao
Publication Title: 
Cell Reports

The NAD(+)-dependent protein deacetylase SIRT6 regulates genome stability, cancer, and lifespan. Mice overexpressing SIRT6 (MOSES) have lower low-density lipoprotein cholesterol levels and are protected against the physiological damage of obesity. Here, we examined the role of SIRT6 in cholesterol regulation via the lipogenic transcription factors SREBP1 and SREBP2, and AMP-activated protein kinase (AMPK). We show that SIRT6 represses SREBP1 and SREBP2 by at least three mechanisms. First, SIRT6 represses the transcription levels of SREBP1/SREBP2 and that of their target genes.

Author(s): 
Elhanati, Sivan
Kanfi, Yariv
Varvak, Alexander
Roichman, Asael
Carmel-Gross, Ilana
Barth, Shaul
Gibor, Gilad
Cohen, Haim Y.

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