HSP27 Heat-Shock Proteins

Publication Title: 
Journal of Biotechnology

It has been widely reported that CHO cells undergo apoptosis in culture, despite supplementation of nutrients through fed-batch strategies. Improvement of cell viability in culture can effectively improve recombinant protein yield through extension of the culture's production lifespan, especially at high cell densities. Heat shock proteins (HSPs) have been reported to demonstrate anti-apoptotic effects against a wide range of physical and chemical stimuli through their ability to bind and act as antagonists to critical apoptotic molecules.

Author(s): 
Lee, Yih Yean
Wong, Kathy T. K.
Tan, Janice
Toh, Poh Choo
Mao, Yanying
Brusic, Vesna
Yap, Miranda G. S.
Publication Title: 
Endocrinology

Beta-cell apoptosis occurs in diabetes mellitus (DM). Heat shock protein (HSP) 27 (human homolog of rodent HSP25) mitigates stress-induced apoptosis but has not been studied in beta-cells. We tested whether HSP27 overexpression attenuates streptozotocin (SZ)-induced DM in vivo and cytokine-induced islet apoptosis in vitro. DM was ascertained by ip glucose tolerance testing, and fasting serum insulin/glucose was measured. Pancreas was stained for insulin, HSP27, and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling, and insulin content was measured.

Author(s): 
Dai, Tiane
Patel-Chamberlin, Mina
Natarajan, Rama
Todorov, Ivan
Ma, Jun
LaPage, Janine
Phillips, Lynetta
Nast, Cynthia C.
Becerra, Diana
Chuang, Peter
Tong, Lili
de Belleroche, Jacqueline
Wells, Dominic J.
Wang, Ying
Adler, Sharon G.
Publication Title: 
Journal of Proteome Research

Oxythiamine (OT), a transketolase inhibitor, is known to inhibit pancreatic cancer cell proliferation. In this study, we investigated the effect of inhibition of the transketolase pathway on signaling pathways in MIA PaCa cancer cells using in-house proteomic techniques. We hypothesized that OT alter protein phosphorylation thus affecting cell cycle arrest and cell proliferation. MIA PaCa-2 cells were cultured in media containing an algal (15)N amino acid mixture at 50% enrichment, with and without OT, to determine protein expression and synthesis.

Author(s): 
Zhang, Hengwei
Cao, Rui
Lee, Wai-Nang Paul
Deng, Caishu
Zhao, Yingchun
Lappe, Joan
Recker, Robert
Yen, Yun
Wang, Qi
Tsai, Ming-Ying
Go, Vay Liang
Xiao, Gary Guishan
Publication Title: 
American Journal of Physiology. Heart and Circulatory Physiology

Therapeutic hypothermia (TH) is a promising cardioprotective treatment for cardiac arrest and acute myocardial infarction, but its cytoprotective mechanisms remain unknown. In this study, we developed a murine cardiomyocyte model of ischemia-reperfusion injury to better determine the mechanisms of TH cardioprotection. We hypothesized that TH manipulates Akt, a survival kinase that mediates mitochondrial protection by modulating reactive oxygen species (ROS) and nitric oxide (NO) generation.

Author(s): 
Shao, Zuo-Hui
Sharp, Willard W.
Wojcik, Kimberly R.
Li, Chang-Qing
Han, Mei
Chang, Wei-Tien
Ramachandran, Srinivasan
Li, Jing
Hamann, Kimm J.
Vanden Hoek, Terry L.
Publication Title: 
BMC complementary and alternative medicine

BACKGROUND: Curcumin has anti-inflammatory, anti-oxidant, and anti-proliferative properties, and depending upon the experimental circumstances, may be pro- or anti-apoptotic. Many of these biological actions could ameliorate diabetic nephropathy. METHODS/DESIGN: Mouse podocytes, cultured in basal or high glucose conditions, underwent acute exposure to curcumin. Western blots for p38-MAPK, COX-2 and cleaved caspase-3; isoelectric focusing for HSP25 phosphorylation; and DNase I assays for F- to G- actin cleavage were performed for in vitro analyses.

Author(s): 
Ma, Jun
Phillips, Lynetta
Wang, Ying
Dai, Tiane
LaPage, Janine
Natarajan, Rama
Adler, Sharon G.
Publication Title: 
Proteomics

Pancreatic cancer is a deadly disease characterized by poor prognosis and patient survival. Green tea polyphenols have been shown to exhibit multiple antitumor activities in various cancers, but studies on the pancreatic cancer are very limited. To identify the cellular targets of green tea action, we exposed a green tea extract (GTE) to human pancreatic ductal adenocarcinoma HPAF-II cells and performed two-dimensional gel electrophoresis of the cell lysates. We identified 32 proteins with significantly altered expression levels.

Author(s): 
Zhang, Lifeng
Pang, Eric
Loo, Rachel R. Ogorzalek
Rao, Jianyu
Go, Vay-Liang W.
Loo, Joseph A.
Lu, Qing-Yi
Publication Title: 
Biological Research

Cimicifuga foetida, an Asian Cimicifuga species, has been employed as a cooling and detoxification agent in traditional Chinese medicine since ancient times. For this herb, two cycloartane triterpene glycosides isolated from the rhizomes have demonstrated cytotoxicity on rat tumor and human cancer cell lines. Since human Hsp27 is increased in various human cancers and exhibits cytoprotective activity that affects tumorigenesis and the susceptibility of tumours to cancer treatment, the purpose of this research was to study the expression of this protein in MCF-7 breast cancer cells.

Author(s): 
Soler, Maritza C.
Molina, Jessica L.
Díaz, Hugo A.
Pinto, Vivian C.
Barrios, Yasenka L.
He, Kan
Roller, Marc
Weinstein-Oppenheimer, Caroline R.
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