HSP70 Heat-Shock Proteins

Publication Title: 
Experimental Cell Research

The lifespan of human foreskin fibroblasts (HFF5), cultured under standard in vitro conditions (including ambient atmospheric oxygen tension), was extended slightly by expression of exogenous mortalin (mot-2)/mthsp70/Grp75, but not by the catalytic subunit of telomerase, hTERT. Together, mot-2 and hTERT permitted bypass of senescence, a substantial extension of lifespan, and possibly immortalization. This is the first demonstration that mot-2 and telomerase can cooperate in the immortalization process.

Author(s): 
Kaul, Sunil C.
Yaguchi, Tomoko
Taira, Kazunari
Reddel, Roger R.
Wadhwa, Renu
Publication Title: 
PloS One

Calorie restriction (CR) produces several health benefits and increases lifespan in many species. Studies suggest that alternate-day fasting (ADF) and exercise can also provide these benefits. Whether CR results in lifespan extension in humans is not known and a direct investigation is not feasible. However, phenotypes observed in CR animals when compared to ad libitum fed (AL) animals, including increased stress resistance and changes in protein expression, can be simulated in cells cultured with media supplemented with blood serum from CR and AL animals.

Author(s): 
Allard, Joanne S.
Heilbronn, Leonie K.
Smith, Carolina
Hunt, Nicole D.
Ingram, Donald K.
Ravussin, Eric
Pennington CALERIE Team
de Cabo, Rafael
Publication Title: 
Journal of Biotechnology

It has been widely reported that CHO cells undergo apoptosis in culture, despite supplementation of nutrients through fed-batch strategies. Improvement of cell viability in culture can effectively improve recombinant protein yield through extension of the culture's production lifespan, especially at high cell densities. Heat shock proteins (HSPs) have been reported to demonstrate anti-apoptotic effects against a wide range of physical and chemical stimuli through their ability to bind and act as antagonists to critical apoptotic molecules.

Author(s): 
Lee, Yih Yean
Wong, Kathy T. K.
Tan, Janice
Toh, Poh Choo
Mao, Yanying
Brusic, Vesna
Yap, Miranda G. S.
Publication Title: 
Mechanisms of Ageing and Development

Heat shock proteins are highly conserved proteins that, when produced intracellularly, protect stress exposed cells. In contrast, extracellular heat shock protein 70 (Hsp70) has been shown to have both protective and deleterious effects. In this study, we assessed heat shock protein 70 for its potential role in human longevity.

Author(s): 
Terry, Dellara F.
Wyszynski, Diego F.
Nolan, Vikki G.
Atzmon, Gil
Schoenhofen, Emily A.
Pennington, JaeMi Y.
Andersen, Stacy L.
Wilcox, Marsha A.
Farrer, Lindsay A.
Barzilai, Nir
Baldwin, Clinton T.
Asea, Alexzander
Publication Title: 
Molecular and Cellular Biology

The AU-rich elements (AREs) encoded within many mRNA 3' untranslated regions (3'UTRs) are targets for factors that control transcript longevity and translational efficiency. Hsp70, best known as a protein chaperone with well-defined peptide-refolding properties, is known to interact with ARE-like RNA substrates in vitro. Here, we show that cofactor-free preparations of Hsp70 form direct, high-affinity complexes with ARE substrates based on specific recognition of U-rich sequences by both the ATP- and peptide-binding domains.

Author(s): 
Kishor, Aparna
Tandukar, Bishal
Ly, Yann V.
Toth, Eric A.
Suarez, Yvelisse
Brewer, Gary
Wilson, Gerald M.
Publication Title: 
The Journal of Nutritional Biochemistry

Aging is associated with a reduced ability to cope with physiological challenges. Although the mechanisms underlying age-related alterations in stress tolerance are not well defined, many studies support the validity of the oxidative stress hypothesis, which suggests that lowered functional capacity in aged organisms is the result of an increased generation of reactive oxygen and nitrogen species.

Author(s): 
Calabrese, Vittorio
Giuffrida Stella, Anna Maria
Calvani, Menotti
Butterfield, D. Allan
Publication Title: 
PloS One

Calorie restriction (CR) produces several health benefits and increases lifespan in many species. Studies suggest that alternate-day fasting (ADF) and exercise can also provide these benefits. Whether CR results in lifespan extension in humans is not known and a direct investigation is not feasible. However, phenotypes observed in CR animals when compared to ad libitum fed (AL) animals, including increased stress resistance and changes in protein expression, can be simulated in cells cultured with media supplemented with blood serum from CR and AL animals.

Author(s): 
Allard, Joanne S.
Heilbronn, Leonie K.
Smith, Carolina
Hunt, Nicole D.
Ingram, Donald K.
Ravussin, Eric
Pennington CALERIE Team
de Cabo, Rafael
Publication Title: 
Science (New York, N.Y.)
Author(s): 
Saunders, Laura R.
Verdin, Eric
Publication Title: 
The American Journal of Chinese Medicine

Lavender essential oil (LEO) is one the most favorite and widely used essential oils in aromatherapy. Many studies have demonstrated its functions in calming, assisting sleep, reducing pain and muscular spasms and its antiseptic function. To date, however, the mechanism of LEO on inflammation response is not well understood. In this study, we examined the effect of LEO on 5 ?g/ml lipopolysaccharide (LPS) induced inflammation reaction in human monocyte THP-1 cells. We found treatment of 0.1% LEO significantly increased cell viability and inhibited the IL-1?

Author(s): 
Huang, Mei-Yu
Liao, May-Hua
Wang, Yang-Kao
Huang, Yung-Sheng
Wen, Hsiao-Chuan
Publication Title: 
PloS One

BACKGROUND AND PURPOSE: Withanolides are naturally occurring chemical compounds. They are secondary metabolites produced via oxidation of steroids and structurally consist of a steroid-backbone bound to a lactone or its derivatives. They are known to protect plants against herbivores and have medicinal value including anti-inflammation, anti-cancer, adaptogenic and anti-oxidant effects. Withaferin A (Wi-A) and Withanone (Wi-N) are two structurally similar withanolides isolated from Withania somnifera, also known as Ashwagandha in Indian Ayurvedic medicine.

Author(s): 
Vaishnavi, Kirti
Saxena, Nishant
Shah, Navjot
Singh, Rumani
Manjunath, Kavyashree
Uthayakumar, M.
Kanaujia, Shankar P.
Kaul, Sunil C.
Sekar, Kanagaraj
Wadhwa, Renu

Pages

Subscribe to RSS - HSP70 Heat-Shock Proteins