Human Umbilical Vein Endothelial Cells

Publication Title: 
Scientific Reports

Vascular endothelial growth factor A (VEGFA) plays an important role in tumour angiogenesis and its angiogenic action is mainly mediated through its VEGF receptor 2 (VEGFR-2). Therefore drugs targeting VEGFA/VEGFR-2 are being presently used in the clinics for treatment of several types of solid malignant tumours. We here in report that low dose of chebulagic acid (CA), a hydrolysable tannin found in myrobalan fruits can inhibit VEGFA induced vascular permeability, endothelial cell proliferation, migration, tube formation and thereby, angiogenesis by suppressing VEGFR-2 phosphorylation.

Author(s): 
Lu, Kai
Basu, Sujit
Publication Title: 
The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences

Insulin-like growth factor 1 (IGF-1) stimulates cell proliferation and is crucial for maintenance of somatic tissues. However, this effect is associated with the inhibition of FOXO transcription factors and downregulation of antioxidative enzymes. In this study, we compared the responses of primary dermal fibroblasts and human umbilical vein endothelial cells with IGF-1 treatment. We found that IGF-1 primarily downregulated enzymatic antioxidants in skin fibroblasts. However, human umbilical vein endothelial cells were protected from an IGF-1-mediated decrease in antioxidative capacity.

Author(s): 
Stone, Rivka C.
Kim, Soyeon
Barnes, Betsy J.
Aviv, Abraham
Publication Title: 
Toxins

Tumor endothelium marker-8 (TEM8) and capillary morphogenesis protein-2 (CMG2) are the two well-characterized anthrax toxin receptors, each containing a von Willebrand factor A (vWA) domain responsible for anthrax protective antigen (PA) binding. Recently, a cell-based analysis was used to implicate another vWA domain-containing protein, integrin ?1 as a third anthrax toxin receptor. To explore whether proteins other than TEM8 and CMG2 function as anthrax toxin receptors in vivo, we challenged mice lacking TEM8 and/or CMG2.

Author(s): 
Liu, Shihui
Zhang, Yi
Hoover, Benjamin
Leppla, Stephen H.
Publication Title: 
BMC complementary and alternative medicine

BACKGROUND: Though Mica, a thin and sheet like mineral, has been used as a mineral medicine for treatment of bleeding, dysentery and inflammation in traditional medicine including Ayurveda, the biological evidences of Mica were not clearly elucidated so far. Thus, in the present study, the antitumor mechanism of particled Mica (STB-HO) was examined in colorectal cancers.

Author(s): 
Cho, Sung-Yun
Lee, Hyo-Jeong
Cho, Sun-Mi
Kim, Bonglee
Jung, Yeon Kwon
Kim, Sung-Hoon
Publication Title: 
The British Journal of Nutrition

Amla (Emblica officinalis Gaertn.) has been used for many centuries in traditional Indian Ayurvedic formulations for the prevention and treatment of many inflammatory diseases. The present study evaluated the anti-inflammatory and anticoagulant properties of amla fruit extract. The amla fruit extract potentially and significantly reduced lipopolysaccharide (LPS)-induced tissue factor expression and von Willebrand factor release in human umbilical vein endothelial cells (HUVEC) in vitro at clinically relevant concentrations (1-100 μg/ml).

Author(s): 
Rao, Theertham Pradyumna
Okamoto, Takayuki
Akita, Nobuyuki
Hayashi, Tatsuya
Kato-Yasuda, Naomi
Suzuki, Koji
Publication Title: 
BMC complementary and alternative medicine

BACKGROUND: Though Mica, a thin and sheet like mineral, has been used as a mineral medicine for treatment of bleeding, dysentery and inflammation in traditional medicine including Ayurveda, the biological evidences of Mica were not clearly elucidated so far. Thus, in the present study, the antitumor mechanism of particled Mica (STB-HO) was examined in colorectal cancers.

Author(s): 
Cho, Sung-Yun
Lee, Hyo-Jeong
Cho, Sun-Mi
Kim, Bonglee
Jung, Yeon Kwon
Kim, Sung-Hoon
Publication Title: 
Archives of Pharmacal Research

Sparstolonin B (SsnB) is an isocoumarin compound isolated from the tubers of both Sparganium stoloniferum and Scirpus yagara. We previously demonstrated that SsnB blocked the Toll-like receptor (TLR) 2- and TLR4-triggered inflammatory signaling in macrophages by inhibiting the recruitment of MyD88 to the TIR domains of TLR2 and TLR4. The present study was designed to examine the effects of SsnB on vascular inflammatory responses in human umbilical vein endothelial cells (HUVECs) challenged by lipopolysaccharide (LPS, a TLR4 ligand).

Author(s): 
Liang, Qiaoli
Yu, Fang
Cui, Xiaodong
Duan, Jin'ao
Wu, Qinan
Nagarkatti, Prakash
Fan, Daping
Publication Title: 
African journal of traditional, complementary, and alternative medicines: AJTCAM / African Networks on Ethnomedicines

Qidantongmai (QDTM) is a Traditional Chinese Medicine (TCM) preparation that has long been used in folk medicine for the treatment of cardiovascular diseases. However, the underlying mechanisms are poorly understood. The present study was designed to determine the effects of QDTM on endothelial cells under hypoxic conditions both in vitro and in vivo. Primary human umbilical vein endothelial cells (HUVECs) were isolated, pretreated with QDTM medicated serum or saline control, and then cultured under hypoxia (2% oxygen) for 24 h.

Author(s): 
Wang, Bing
Wang, Wen
Li, Feng
Wang, Zongren
Ma, Jing
Zhao, Gang
Publication Title: 
Circulation Journal: Official Journal of the Japanese Circulation Society

BACKGROUND: Hexosamine biosynthetic pathway (HBP) is implicated in increased plasminogen activator inhibitor-1 (PAI-1), and endothelial nitric oxide synthase (eNOS) dysfunction in diabetes. Glucosamine (GlcN) that directly activates HBP is a dietary supplement and is clinically used to treat osteoarthritis despite uncertain efficacy and adverse cardiovascular effects observed in animal models. p38 mitogen-activated protein kinase (p38mapk) has been shown to be involved in HBP-mediated biological processes.

Author(s): 
Wu, Zongsong
Xiong, Yuyan
Gajanayake, Thusitha
Ming, Xiu-Fen
Yang, Zhihong
Publication Title: 
Zhong Xi Yi Jie He Xue Bao = Journal of Chinese Integrative Medicine

OBJECTIVE: To observe the inhibitory effects of Feiyanning Decoction, a compound traditional Chinese herbal medicine for replenishing qi, nourishing essence, and diminishing stagnation by detoxification, on proliferation, migration and tube formation of human umbilical vein endothelial cells (HUVECs). METHODS: HUVECs were isolated from the new-born umbilical core of human. Serum containing Feiyanning was prepared in SD rats by oral gavage of the Feiyanning Decoction for three days.

Author(s): 
Deng, Hai-bin
Wang, Zhong-qi
Xu, Zhen-ye

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