Hydrogen-Ion Concentration

Publication Title: 
Biotechnology and Applied Biochemistry

Statistically based experimental designs were applied to the optimization of cultural conditions for tannase production, an enzyme of great importance, from Penicillium variable. First, D-optimal design was used to evaluate the effects of variables, including concentrations of substrate (chebulic myrobalan, fruits of the tree Terminalia chebula ), pH, inoculum density, agitation and incubation period, on tannase production. The optimum value of pH and inoculum density thus obtained was 5.0 and 5 x 10(7) spores/50 ml respectively.

Author(s): 
Saxena, Shashi
Saxena, Rajendra K.
Publication Title: 
Journal of Basic Microbiology

Modified solid-state fermentation (MSSF) of tannin-rich substrates for production of tannase and gallic acid was carried out using two fungal cultures, Rhizopus oryzae (RO IIT RB-13, NRRL 21498) and Aspergillus foetidus (GMRB013 MTCC 3557). The tannin rich substrates included powdered fruits of Terminalia chebula and Caesalpinia digyna pod cover powder. The different environmental parameters for the maximum production of tannase and gallic acid were optimized through media engineering.

Author(s): 
Mukherjee, Gargi
Banerjee, Rintu
Publication Title: 
Bioresource Technology

Modified solid-state fermentation (MSSF) of tannin-rich substrate yielding tannase and gallic acid was carried out using a co-culture of the filamentous fungi, Rhizopus oryzae (RO IIT RB-13, NRRL 21498) and Aspergillus foetidus (GMRB013 MTCC 3557). Powdered fruits of Terminalia chebula and powdered pod cover of Caesalpinia digyna was used in the process and the different process parameters for maximum production of tannase and gallic acid by co-culture method were optimized through media engineering. MSSF was carried out at the optimum conditions of 30 degrees C and 80% relative humidity.

Author(s): 
Banerjee, Rintu
Mukherjee, Gargi
Patra, Krushna Chandra
Publication Title: 
Indian Journal of Dental Research: Official Publication of Indian Society for Dental Research

Plant-derived medicines have been a part of our traditional health care system, and the antimicrobial properties of plant-derived compounds are well documented. The purpose of this study is to evaluate the effect of an aqueous extract of Terminalia chebula (a medicinal plant) on salivary samples and its potential for use as an anticaries agent in the form of mouthwash. A concentrated aqueous extract was prepared from the fruit of T. chebula . A mouth rinse of 10% concentration was prepared by diluting the extract in sterile distilled water.

Author(s): 
Carounanidy, Usha
Satyanarayanan, R.
Velmurugan, A.
Publication Title: 
Journal of Food Science

Triphala, a mixture of Emblica officinalis, Terminalia chebula, and Terminalia bellirica, containing ingredients from plant origin, is often prone to microbial contamination. A high level of microbial contamination was observed in Triphala samples obtained from different sources. On gamma radiation processing, a sharp decline in log CFU was observed with increasing radiation dose and a complete decontamination at 5 kGy. Average D10 value for total aerobic and fungal counts were observed to be 0.55 +/- 0.073 kGy and 0.94 +/- 0.043 kGy, respectively.

Author(s): 
Kumari, N.
Kumar, P.
Mitra, D.
Prasad, B.
Tiwary, B. N.
Varshney, L.
Publication Title: 
Oral Health & Preventive Dentistry

PURPOSE: The aim of the present study was to assess the effectiveness of Terminalia chebula aqueous extract rinse on salivary pH and Streptococcus mutans. Mouthrinses have been in use from time immemorial as a supplement for routine oral hygiene practice. Although a large number of mouthrinses are currently available, many of them possess certain drawbacks, which has necessitated the search for alternate agents. MATERIALS AND METHODS: Ten per cent of T. chebula extract was prepared. A purposive sample of 30 subjects was selected and randomly divided into extract and control groups.

Author(s): 
Nayak, Sushma S.
Kumar, B. R. Ashok
Ankola, Anil V.
Hebbal, Mamata
Publication Title: 
Applied Biochemistry and Biotechnology

Sol/gel-derived silica gel was prepared at room temperature from tetraethyl orthosilicate precursor. The extracts of Terminalia chebula (Haritoki) were entrapped into the porous silica gel. Fourier transform infrared analysis revealed the proper adsorption of herbal values in the nanopores of the silica gel. Porosity was estimated by transmission electron microscope studies. The release kinetics of the extract in both 0.1 N HCl, pH 1.2, and Phosphate-buffer saline (PBS), pH 7.2, were determined using UV-Vis spectroscopy.

Author(s): 
Chakraborty, Suparna
Mitra, Manoj Kumar
Chaudhuri, Mahua Ghosh
Sa, Biswanath
Das, Satadal
Dey, Rajib
Publication Title: 
Phytomedicine: International Journal of Phytotherapy and Phytopharmacology

In continuation of our drug discovery program on Indian medicinal plants, the gastro protective mechanism of chebulinic acid isolated from Terminalia chebula fruit was investigated. Chebulinic acid was evaluated against cold restraint (CRU), aspirin (AS), alcohol (AL) and pyloric ligation (PL) induced gastric ulcer models in rats. Potential anti-ulcer activity of chebulinic acid was observed against CRU (62.9%), AS (55.3%), AL (80.67%) and PL (66.63%) induced ulcer models.

Author(s): 
Mishra, Vaibhav
Agrawal, Manali
Onasanwo, Samuel Adetunji
Madhur, Gaurav
Rastogi, Preeti
Pandey, Haushila Prasad
Palit, Gautam
Narender, Tadigoppula
Publication Title: 
Phytotherapy research: PTR

A double blind, randomized, controlled study with three parallel treatment groups was done to evaluate the efficacy of a Terminalia chebula 10% mouth rinse compared with chlorhexidine 0.12% mouth rinse, applied two times daily for 2 weeks, in the treatment of dental plaque and gingivitis. Seventy-eight patients were included in the study. The efficacy variables were periodontal indices on days 0, 7 and 14 after commencement of therapy. Twenty six patients received chlorhexidine mouth rinse, twenty six Terminalia chebula mouth rinse and twenty six received saline solution.

Author(s): 
Gupta, Devanand
Bhaskar, D. J.
Gupta, Rajendra Kumar
Karim, Bushra
Gupta, Vipul
Punia, Himanshu
Batra, Manu
Jain, Ankita
Agarwal, Amit
Singh, Pradeep
Publication Title: 
Journal of Clinical Apheresis

Extension of the storage period of apheresis platelets to seven or ten days may be possible with the implementation of screening for bacteria. This, however, may impair platelet quality, and additive compounds that improve storage parameters would be desirable. Apheresis platelets were harvested using the Cobe LRS device. Part of the product was aliquoted into two CLX bags, 60 ml into each, on day 0. L-carnitine (LC) to a final concentration of 5 mM was added to one container and saline to the other.

Author(s): 
Sweeney, Joseph D.
Kouttab, Nicola M.
Arduini, Arduino

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