Immunoenzyme Techniques

Publication Title: 
Experimental & Molecular Medicine

Neural progenitor cells (NPs) have shown several promising benefits for the treatment of neurological disorders. To evaluate the therapeutic potential of human neural progenitor cells (hNPs) in amyotrophic lateral sclerosis (ALS), we transplanted hNPs or growth factor (GF)-expressing hNPs into the central nervous system (CNS) of mutant Cu/Zn superoxide dismutase (SOD1(G93A)) transgenic mice.

Author(s): 
Park, Sungju
Kim, Hyoung-Tae
Yun, Seokkwan
Kim, Il-Sun
Lee, Jiyoon
Lee, Il-Shin
Park, Kook In
Publication Title: 
Free Radical Biology & Medicine

Steroid hormones exhibit diverse biological activities. Despite intensive studies on steroid function at the genomic level, their nongenomic actions remain an enigma. In this study, we investigated the role of reactive oxygen species (ROS) in androgen-stimulated prostate cancer (PCa) cell proliferation. In androgen-treated PCa cells, increased cell growth and ROS production correlated with elevated p66Shc protein, an authentic oxidase. This growth stimulation was blocked by antioxidants.

Author(s): 
Veeramani, Suresh
Chou, Yu-Wei
Lin, Frank C.
Muniyan, Sakthivel
Lin, Fen-Fen
Kumar, Satyendra
Xie, Yan
Lele, Subodh M.
Tu, Yaping
Lin, Ming-Fong
Publication Title: 
Developmental Cell

Developmental timing genes catalyze stem cell progression and animal maturation programs across taxa. Caenorhabditis elegans DRE-1/FBXO11 functions in an SCF E3-ubiquitin ligase complex to regulate the transition to adult programs, but its cognate proteolytic substrates are unknown. Here, we identify the conserved transcription factor BLMP-1 as a substrate of the SCF(DRE-1/FBXO11) complex. blmp-1 deletion suppressed dre-1 mutant phenotypes and exhibited developmental timing defects opposite to dre-1.

Author(s): 
Horn, Moritz
Geisen, Christoph
Cermak, Lukas
Becker, Ben
Nakamura, Shuhei
Klein, Corinna
Pagano, Michele
Antebi, Adam
Publication Title: 
Neuro-Oncology

BACKGROUND: A proportion of glioblastoma stemlike cells (GSCs) expressing endothelial cell marker CDH5 (vascular-endothelial-cadherin or CD144) can transdifferentiate into endothelial cells and form blood vessels. However, the implications of CDH5 expression in gliomas and how it is regulated in GSCs remain to be clarified. METHODS: The mRNA and protein levels of CDH5 were detected in glioma samples and cultured cell lines, and the prognostic value of the CDH5 expression level for GBM patients was evaluated.

Author(s): 
Mao, Xing-Gang
Xue, Xiao-Yan
Wang, Liang
Zhang, Xiang
Yan, Ming
Tu, Yan-yang
Lin, Wei
Jiang, Xiao-Fan
Ren, Hong-Gang
Zhang, Wei
Song, Shao-Jun
Publication Title: 
Cancer Prevention Research (Philadelphia, Pa.)

Chemoprevention of head and neck squamous cell carcinoma (HNSCC), a disease associated with high mortality rates and frequent occurrence of second primary tumor (SPT), is an important clinical goal. The epidermal growth factor receptor (EGFR)-signal transducer and activator of transcription (STAT)-3 signaling pathway is known to play a key role in HNSCC growth, survival, and prognosis, thereby serving as a potential therapeutic target in the treatment of HNSCC.

Author(s): 
Leeman-Neill, Rebecca J.
Seethala, Raja R.
Singh, Shivendra V.
Freilino, Maria L.
Bednash, Joseph S.
Thomas, Sufi M.
Panahandeh, Mary C.
Gooding, William E.
Joyce, Sonali C.
Lingen, Mark W.
Neill, Daniel B.
Grandis, Jennifer R.
Publication Title: 
Cancer Prevention Research (Philadelphia, Pa.)

Prostate cancer remains the second leading cause of cancer deaths among American men. Earlier diagnosis increases survival rate in patients. However, treatments for advanced disease are limited to hormone ablation techniques and palliative care. Thus, new methods of treatment and prevention are necessary for inhibiting disease progression to a hormone refractory state. One of the approaches to control prostate cancer is prevention through diet, which inhibits one or more neoplastic events and reduces the cancer risk.

Author(s): 
Ru, Peng
Steele, Robert
Nerurkar, Pratibha V.
Phillips, Nancy
Ray, Ratna B.
Publication Title: 
Cancer Prevention Research (Philadelphia, Pa.)

Prostate cancer remains the second leading cause of cancer deaths among American men. Earlier diagnosis increases survival rate in patients. However, treatments for advanced disease are limited to hormone ablation techniques and palliative care. Thus, new methods of treatment and prevention are necessary for inhibiting disease progression to a hormone refractory state. One of the approaches to control prostate cancer is prevention through diet, which inhibits one or more neoplastic events and reduces the cancer risk.

Author(s): 
Ru, Peng
Steele, Robert
Nerurkar, Pratibha V.
Phillips, Nancy
Ray, Ratna B.
Publication Title: 
Cancer Prevention Research (Philadelphia, Pa.)

Chemoprevention of head and neck squamous cell carcinoma (HNSCC), a disease associated with high mortality rates and frequent occurrence of second primary tumor (SPT), is an important clinical goal. The epidermal growth factor receptor (EGFR)-signal transducer and activator of transcription (STAT)-3 signaling pathway is known to play a key role in HNSCC growth, survival, and prognosis, thereby serving as a potential therapeutic target in the treatment of HNSCC.

Author(s): 
Leeman-Neill, Rebecca J.
Seethala, Raja R.
Singh, Shivendra V.
Freilino, Maria L.
Bednash, Joseph S.
Thomas, Sufi M.
Panahandeh, Mary C.
Gooding, William E.
Joyce, Sonali C.
Lingen, Mark W.
Neill, Daniel B.
Grandis, Jennifer R.
Publication Title: 
Cancer Prevention Research (Philadelphia, Pa.)

Prostate cancer remains the second leading cause of cancer deaths among American men. Earlier diagnosis increases survival rate in patients. However, treatments for advanced disease are limited to hormone ablation techniques and palliative care. Thus, new methods of treatment and prevention are necessary for inhibiting disease progression to a hormone refractory state. One of the approaches to control prostate cancer is prevention through diet, which inhibits one or more neoplastic events and reduces the cancer risk.

Author(s): 
Ru, Peng
Steele, Robert
Nerurkar, Pratibha V.
Phillips, Nancy
Ray, Ratna B.
Publication Title: 
Clinical Chemistry

BACKGROUND: Oxidative stress is pivotal in atherogenesis. Although the most widely used indirect assay to quantify oxidative stress is LDL oxidative susceptibility, direct assays such as urinary F(2)-isoprostanes have shown great promise. METHODS: We evaluated the utility of both a direct measure of oxidative stress (urinary F(2)-isoprostanes) and an indirect measure of copper-catalyzed, LDL oxidation in a model of increased oxidative stress (diabetes). We also evaluated an enzyme immunoassay (EIA) method for urinary F(2)-isoprostanes with a gas chromatography-mass spectrometry method.

Author(s): 
Devaraj, S.
Hirany, S. V.
Burk, R. F.
Jialal, I.

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