Inhibitor of Apoptosis Proteins

Publication Title: 
Clinical Cancer Research: An Official Journal of the American Association for Cancer Research

PURPOSE: To assess the efficacy of rapamycin treatment in chemoprevention and chemotherapy of tumorigenesis in a genetically defined mouse model of head and neck squamous cell carcinoma (HNSCC). Experimental design: Knockdown of Tgfbr1 and/or Pten using siRNA-mediated RNA interference was carried out in human HNSCC cell lines to analyze molecular changes in the mTOR pathway. Tgfbr1(flox/flox); Pten(flox/flox); K14-CreER(tam) mice were treated with oral gavage of tamoxifen for the conditional deletion of Tgfbr1 and Pten in oral mucosa, resulting in HNSCC.

Author(s): 
Sun, Zhi-Jun
Zhang, Lu
Hall, Bradford
Bian, Yansong
Gutkind, J. Silvio
Kulkarni, Ashok B.
Publication Title: 
Archives of Pathology & Laboratory Medicine

CONTEXT: Human telomerase reverse transcriptase (hTERT), an enzyme that enables cells to overcome replicative senescence and to divide indefinitely, is overexpressed in many cancers and their precursor lesions. OBJECTIVE: To test whether hTERT expression is related to neoplastic progression and resistance to apoptosis in vulvar epithelia. DESIGN: Immunoexpression of hTERT was evaluated in 101 formalin-fixed, paraffin-embedded archival vulvar epithelia consisting of normal squamous vulvar epithelia (n ?=? 25), lichen sclerosus (n ?=?

Author(s): 
Wellenhofer, Alfred
Brustmann, Hermann
Publication Title: 
Journal of Zhejiang University. Science. B

This paper aims to investigate the effects of artesunate (ART) on growth and apoptosis in human osteosarcoma HOS cell line in vitro and in vivo and to explore the possible underlying mechanisms. Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The induction of apoptosis was detected by light and transmission electron microscopy and flow cytometry. Western blot analysis was used to investigate the related mechanisms. Nude mice were further employed to investigate the antitumour activity of ART in vivo.

Author(s): 
Xu, Qiang
Li, Zhao-Xu
Peng, Hui-Qin
Sun, Zheng-Wang
Cheng, Rui-Lin
Ye, Zhao-Ming
Li, Wei-Xu
Publication Title: 
International Journal of Nanomedicine

The present study evaluated whether the magnetic nanoparticles of Fe(3)O(4) (MNPs-Fe(3)O(4)) could enhance the activity of artesunate (ART), and to explore its potential mechanisms. Cytotoxicity of the copolymer of ART with MNPs-Fe(3)O(4) on K562 cells was detected by MTT assay and the apoptosis rate of K562 cells was measured by flow cytometry. Protein expression levels of bcl-2, bax, bcl-rambo, caspase-3, and survivin in K562 cells were measured by Western blot.

Author(s): 
Wang, Ying
Han, Yuxiang
Yang, Yingying
Yang, Jingci
Guo, Xiaonan
Zhang, Jingnan
Pan, Ling
Xia, Guohua
Chen, Baoan
Publication Title: 
Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University

OBJECTIVE: To construct recombinant expression vectors of small interfering RNA (siRNA) targeting survivin and investigate apoptosis of glioma cell line U251 mediated by the survivin-targeting siRNA. METHODS: According to the sequence of the coding region of survivin gene, two strings of 19 nucleotides of inverted sequence flanking the loop sequence of two complementary 9-base oligonucleotides were designed and synthesized to form hairpin construct as the DNA templates for the target siRNA.

Author(s): 
Xu, Ru-xiang
Tu, Yan-yang
Jiang, Xiao-dan
Feng, Jiang-nan
Huang, Jun
Publication Title: 
Oncogene

Human differentially expressed in chondrocytes (DEC), mouse stimulated with retinoic acid and rat split and hairy related proteins constitute a structurally distinct class of the basic helix-loop-helix proteins. DEC1 is abundantly expressed in tumors and protects against apoptosis induced by serum starvation. In this study, we report that DEC1 antiapoptosis is achieved by inducing survivin, an antiapoptotic protein. In paired tumor-normal tissues, survivin and DEC1 exhibited a paralleled expression pattern.

Author(s): 
Li, Y.
Xie, M.
Yang, J.
Yang, D.
Deng, R.
Wan, Y.
Yan, B.
Publication Title: 
Molecular Nutrition & Food Research

SCOPE: Epidemiologic evidence suggests diets rich in cruciferous vegetables, particularly broccoli, are associated with lower bladder cancer risk. Our objectives are to investigate these observations and determine the role of isothiocyanates in primary or secondary bladder cancer prevention. METHODS AND RESULTS: We initially investigate the mechanisms whereby broccoli and broccoli sprout extracts and pure isothiocyanates inhibit normal, noninvasive (RT4), and invasive (J82, UMUC3) human urothelial cell viability.

Author(s): 
Abbaoui, Besma
Riedl, Kenneth M.
Ralston, Robin A.
Thomas-Ahner, Jennifer M.
Schwartz, Steven J.
Clinton, Steven K.
Mortazavi, Amir
Publication Title: 
International Journal of Cancer. Journal International Du Cancer

Survivin protein is an attractive candidate for cancer immunotherapy since it is abundantly expressed in most common human cancers and mostly absent in normal adult tissues. Malignant mesothelioma (MM) is a deadly cancer associated with asbestos or erionite exposure for which no successful therapies are currently available.

Author(s): 
Bertino, Pietro
Panigada, Maddalena
Soprana, Elisa
Bianchi, Valentina
Bertilaccio, Sabrina
Sanvito, Francesca
Rose, Aaron H.
Yang, Haining
Gaudino, Giovanni
Hoffmann, Peter R.
Siccardi, Antonio
Carbone, Michele
Publication Title: 
Cancer Research

Breast cancer is one of the most common cancers among women in the United States. Although there are effective drugs for treating advanced stages of breast cancers, women eventually develop resistance. One of the approaches to control breast cancer is prevention through diet, which inhibits one or more neoplastic events and reduces cancer risk. In this study, we have used human breast cancer cells, MCF-7 and MDA-MB-231, and primary human mammary epithelial cells as an in vitro model to assess the efficacy of bitter melon (Momordica charantia) extract (BME) as an anticancer agent.

Author(s): 
Ray, Ratna B.
Raychoudhuri, Amit
Steele, Robert
Nerurkar, Pratibha
Publication Title: 
Cancer Research

Breast cancer is one of the most common cancers among women in the United States. Although there are effective drugs for treating advanced stages of breast cancers, women eventually develop resistance. One of the approaches to control breast cancer is prevention through diet, which inhibits one or more neoplastic events and reduces cancer risk. In this study, we have used human breast cancer cells, MCF-7 and MDA-MB-231, and primary human mammary epithelial cells as an in vitro model to assess the efficacy of bitter melon (Momordica charantia) extract (BME) as an anticancer agent.

Author(s): 
Ray, Ratna B.
Raychoudhuri, Amit
Steele, Robert
Nerurkar, Pratibha

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